Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos

Abstract MicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here...

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Autores principales:	Ying Wu, Qi-Yong Lou, Feng Ge, Qian Xiong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc557a13cd264f4a9ffcf053c01d9e33
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spelling	oai:doaj.org-article:bc557a13cd264f4a9ffcf053c01d9e332021-12-02T15:06:23ZQuantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos10.1038/s41598-017-04166-x2045-2322https://doaj.org/article/bc557a13cd264f4a9ffcf053c01d9e332017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04166-xhttps://doaj.org/toc/2045-2322Abstract MicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here a tandem isobaric mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-21-regulated proteins. A total of 251 proteins were dysregulated after miR-21 knockdown, suggesting that they may be regulated by miR-21. Bioinformatics analysis indicated that these differentially expressed proteins (DEPs) participate in various biological processes, suggesting that miR-21 may be involved in diverse cellular pathways. Sixteen DEPs were also predicted to be miR-21 targets by at least two algorithms, and several candidate target genes were selected for further luciferase reporter analysis. The results showed that genes encoding tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) are direct miR-21 targets. Taken together, our results not only reveal a large number of novel miR-21 regulated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of miR-21 regulation of zebrafish cardiac valvulogenesis and embryonic development.Ying WuQi-Yong LouFeng GeQian XiongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Wu Qi-Yong Lou Feng Ge Qian Xiong Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
description	Abstract MicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here a tandem isobaric mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-21-regulated proteins. A total of 251 proteins were dysregulated after miR-21 knockdown, suggesting that they may be regulated by miR-21. Bioinformatics analysis indicated that these differentially expressed proteins (DEPs) participate in various biological processes, suggesting that miR-21 may be involved in diverse cellular pathways. Sixteen DEPs were also predicted to be miR-21 targets by at least two algorithms, and several candidate target genes were selected for further luciferase reporter analysis. The results showed that genes encoding tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) are direct miR-21 targets. Taken together, our results not only reveal a large number of novel miR-21 regulated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of miR-21 regulation of zebrafish cardiac valvulogenesis and embryonic development.
format	article
author	Ying Wu Qi-Yong Lou Feng Ge Qian Xiong
author_facet	Ying Wu Qi-Yong Lou Feng Ge Qian Xiong
author_sort	Ying Wu
title	Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
title_short	Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
title_full	Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
title_fullStr	Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
title_full_unstemmed	Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
title_sort	quantitative proteomics analysis reveals novel targets of mir-21 in zebrafish embryos
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc557a13cd264f4a9ffcf053c01d9e33
work_keys_str_mv	AT yingwu quantitativeproteomicsanalysisrevealsnoveltargetsofmir21inzebrafishembryos AT qiyonglou quantitativeproteomicsanalysisrevealsnoveltargetsofmir21inzebrafishembryos AT fengge quantitativeproteomicsanalysisrevealsnoveltargetsofmir21inzebrafishembryos AT qianxiong quantitativeproteomicsanalysisrevealsnoveltargetsofmir21inzebrafishembryos
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Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos

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