Considering the Lives of Microbes in Microbial Communities

ABSTRACT Over the last decades, sequencing technologies have transformed our ability to investigate the composition and functional capacity of microbial communities. Even so, critical questions remain about these complex systems that cannot be addressed by the bulk, community-averaged data typically...

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Autor principal:	Elizabeth A. Shank
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Bacillus subtilis bacterial heterogeneity cell-cell communication microbial communities microcosm secondary metabolism Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bc5b739d1bff44d3a4f07bd1d066e23d
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spelling	oai:doaj.org-article:bc5b739d1bff44d3a4f07bd1d066e23d2021-12-02T18:44:38ZConsidering the Lives of Microbes in Microbial Communities10.1128/mSystems.00155-172379-5077https://doaj.org/article/bc5b739d1bff44d3a4f07bd1d066e23d2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00155-17https://doaj.org/toc/2379-5077ABSTRACT Over the last decades, sequencing technologies have transformed our ability to investigate the composition and functional capacity of microbial communities. Even so, critical questions remain about these complex systems that cannot be addressed by the bulk, community-averaged data typically provided by sequencing methods. In this Perspective, I propose that future advances in microbiome research will emerge from considering “the lives of microbes”: we need to create methods to explicitly interrogate how microbes exist and interact in native-setting-like microenvironments. This approach includes developing approaches that expose the phenotypic heterogeneity of microbes; exploring the effects of coculture cues on cellular differentiation and metabolite production; and designing visualization systems that capture features of native microbial environments while permitting the nondestructive observation of microbial interactions over space and time with single-cell resolution.Elizabeth A. ShankAmerican Society for MicrobiologyarticleBacillus subtilisbacterial heterogeneitycell-cell communicationmicrobial communitiesmicrocosmsecondary metabolismMicrobiologyQR1-502ENmSystems, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus subtilis bacterial heterogeneity cell-cell communication microbial communities microcosm secondary metabolism Microbiology QR1-502
spellingShingle	Bacillus subtilis bacterial heterogeneity cell-cell communication microbial communities microcosm secondary metabolism Microbiology QR1-502 Elizabeth A. Shank Considering the Lives of Microbes in Microbial Communities
description	ABSTRACT Over the last decades, sequencing technologies have transformed our ability to investigate the composition and functional capacity of microbial communities. Even so, critical questions remain about these complex systems that cannot be addressed by the bulk, community-averaged data typically provided by sequencing methods. In this Perspective, I propose that future advances in microbiome research will emerge from considering “the lives of microbes”: we need to create methods to explicitly interrogate how microbes exist and interact in native-setting-like microenvironments. This approach includes developing approaches that expose the phenotypic heterogeneity of microbes; exploring the effects of coculture cues on cellular differentiation and metabolite production; and designing visualization systems that capture features of native microbial environments while permitting the nondestructive observation of microbial interactions over space and time with single-cell resolution.
format	article
author	Elizabeth A. Shank
author_facet	Elizabeth A. Shank
author_sort	Elizabeth A. Shank
title	Considering the Lives of Microbes in Microbial Communities
title_short	Considering the Lives of Microbes in Microbial Communities
title_full	Considering the Lives of Microbes in Microbial Communities
title_fullStr	Considering the Lives of Microbes in Microbial Communities
title_full_unstemmed	Considering the Lives of Microbes in Microbial Communities
title_sort	considering the lives of microbes in microbial communities
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/bc5b739d1bff44d3a4f07bd1d066e23d
work_keys_str_mv	AT elizabethashank consideringthelivesofmicrobesinmicrobialcommunities
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Considering the Lives of Microbes in Microbial Communities

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