Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix

Abstract 3D cell culture is a helpful approach to study cell-cell interaction in a native-like environment, but is often limited due the challenge of retrieving cells from the material. In this study, we present the use of recombinant lectin B, a sugar-binding protein with four binding cavities, to...

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Autores principales:	Nicholas Bodenberger, Dennis Kubiczek, Laura Trösch, Ali Gawanbacht, Susanne Wilhelm, Denis Tielker, Frank Rosenau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc5ce010ca0d40bcaba77fb0f1cc3d0f
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spelling	oai:doaj.org-article:bc5ce010ca0d40bcaba77fb0f1cc3d0f2021-12-02T12:32:18ZLectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix10.1038/s41598-017-06240-w2045-2322https://doaj.org/article/bc5ce010ca0d40bcaba77fb0f1cc3d0f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06240-whttps://doaj.org/toc/2045-2322Abstract 3D cell culture is a helpful approach to study cell-cell interaction in a native-like environment, but is often limited due the challenge of retrieving cells from the material. In this study, we present the use of recombinant lectin B, a sugar-binding protein with four binding cavities, to enable reversible cell integration into a macroporous protein hydrogel matrix. By functionalizing hydrogel precursors with saccharose, lectin B can both bind to sugar moieties on the cellular surface as well as to the modified hydrogel network. Confocal microscopy and flow cytometry analysis revealed cells to be integrated into the network and to adhere and proliferate. Furthermore, the specificity and reversibility was investigated by using a recombinantly produced yellow fluorescent - lectin B fusion protein and a variety of sugars with diverging affinities for lectin B at different concentrations and elution times. Cells could be eluted within minutes by addition of L-fucose to the cell-loaded hydrogels to make cells available for further analysis.Nicholas BodenbergerDennis KubiczekLaura TröschAli GawanbachtSusanne WilhelmDenis TielkerFrank RosenauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicholas Bodenberger Dennis Kubiczek Laura Trösch Ali Gawanbacht Susanne Wilhelm Denis Tielker Frank Rosenau Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
description	Abstract 3D cell culture is a helpful approach to study cell-cell interaction in a native-like environment, but is often limited due the challenge of retrieving cells from the material. In this study, we present the use of recombinant lectin B, a sugar-binding protein with four binding cavities, to enable reversible cell integration into a macroporous protein hydrogel matrix. By functionalizing hydrogel precursors with saccharose, lectin B can both bind to sugar moieties on the cellular surface as well as to the modified hydrogel network. Confocal microscopy and flow cytometry analysis revealed cells to be integrated into the network and to adhere and proliferate. Furthermore, the specificity and reversibility was investigated by using a recombinantly produced yellow fluorescent - lectin B fusion protein and a variety of sugars with diverging affinities for lectin B at different concentrations and elution times. Cells could be eluted within minutes by addition of L-fucose to the cell-loaded hydrogels to make cells available for further analysis.
format	article
author	Nicholas Bodenberger Dennis Kubiczek Laura Trösch Ali Gawanbacht Susanne Wilhelm Denis Tielker Frank Rosenau
author_facet	Nicholas Bodenberger Dennis Kubiczek Laura Trösch Ali Gawanbacht Susanne Wilhelm Denis Tielker Frank Rosenau
author_sort	Nicholas Bodenberger
title	Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
title_short	Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
title_full	Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
title_fullStr	Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
title_full_unstemmed	Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
title_sort	lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc5ce010ca0d40bcaba77fb0f1cc3d0f
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Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix

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