Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.

<h4>Background</h4>Non thermal Irreversible electroporation (NTIRE) is a new tissue ablation method that induces selective damage only to the cell membrane while sparing all other tissue components. Our group has recently showed that NTIRE attenuated neointimal formation in rodent model....

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Autores principales:	Elad Maor, Antoni Ivorra, Boris Rubinsky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc5f10a96dd84e1fa3229b768c8ce7f5
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spelling	oai:doaj.org-article:bc5f10a96dd84e1fa3229b768c8ce7f52021-11-25T06:16:51ZNon thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.1932-620310.1371/journal.pone.0004757https://doaj.org/article/bc5f10a96dd84e1fa3229b768c8ce7f52009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19270746/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Non thermal Irreversible electroporation (NTIRE) is a new tissue ablation method that induces selective damage only to the cell membrane while sparing all other tissue components. Our group has recently showed that NTIRE attenuated neointimal formation in rodent model. The goal of this study was to determine optimal values of NTIRE for vascular smooth muscle cell (VSMC) ablation.<h4>Methods and results</h4>33 Sprague-Dawley rats were used to compare NTIRE protocols. Each animal had NTIRE applied to its left common carotid artery using a custom-made electrodes. The right carotid artery was used as control. Electric pulses of 100 microseconds were used. Eight IRE protocols were compared: 1-4) 10 pulses at a frequency of 10 Hz with electric fields of 3500, 1750, 875 and 437.5 V/cm and 5-8) 45 and 90 pulses at a frequency of 1 Hz with electric fields of 1750 and 875 V/cm. Animals were euthanized after one week. Histological analysis included VSMC counting and morphometry of 152 sections. Selective slides were stained with elastic Van Gieson and Masson trichrome to evaluate extra-cellular structures. The most efficient protocols were 10 pulses of 3500 V/cm at a frequency of 10 Hz and 90 pulses of 1750 V/cm at a frequency of 1 Hz, with ablation efficiency of 89+/-16% and 94+/-9% respectively. Extra-cellular structures were not damaged and the endothelial layer recovered completely.<h4>Conclusions</h4>NTIRE is a promising, efficient and simple novel technology for VMSC ablation. It enables ablation within seconds without causing damage to extra-cellular structures, thus preserving the arterial scaffold and enabling endothelial regeneration. This study provides scientific information for future anti-restenosis experiments utilizing NTIRE.Elad MaorAntoni IvorraBoris RubinskyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4757 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elad Maor Antoni Ivorra Boris Rubinsky Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
description	<h4>Background</h4>Non thermal Irreversible electroporation (NTIRE) is a new tissue ablation method that induces selective damage only to the cell membrane while sparing all other tissue components. Our group has recently showed that NTIRE attenuated neointimal formation in rodent model. The goal of this study was to determine optimal values of NTIRE for vascular smooth muscle cell (VSMC) ablation.<h4>Methods and results</h4>33 Sprague-Dawley rats were used to compare NTIRE protocols. Each animal had NTIRE applied to its left common carotid artery using a custom-made electrodes. The right carotid artery was used as control. Electric pulses of 100 microseconds were used. Eight IRE protocols were compared: 1-4) 10 pulses at a frequency of 10 Hz with electric fields of 3500, 1750, 875 and 437.5 V/cm and 5-8) 45 and 90 pulses at a frequency of 1 Hz with electric fields of 1750 and 875 V/cm. Animals were euthanized after one week. Histological analysis included VSMC counting and morphometry of 152 sections. Selective slides were stained with elastic Van Gieson and Masson trichrome to evaluate extra-cellular structures. The most efficient protocols were 10 pulses of 3500 V/cm at a frequency of 10 Hz and 90 pulses of 1750 V/cm at a frequency of 1 Hz, with ablation efficiency of 89+/-16% and 94+/-9% respectively. Extra-cellular structures were not damaged and the endothelial layer recovered completely.<h4>Conclusions</h4>NTIRE is a promising, efficient and simple novel technology for VMSC ablation. It enables ablation within seconds without causing damage to extra-cellular structures, thus preserving the arterial scaffold and enabling endothelial regeneration. This study provides scientific information for future anti-restenosis experiments utilizing NTIRE.
format	article
author	Elad Maor Antoni Ivorra Boris Rubinsky
author_facet	Elad Maor Antoni Ivorra Boris Rubinsky
author_sort	Elad Maor
title	Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
title_short	Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
title_full	Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
title_fullStr	Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
title_full_unstemmed	Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
title_sort	non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/bc5f10a96dd84e1fa3229b768c8ce7f5
work_keys_str_mv	AT eladmaor nonthermalirreversibleelectroporationnoveltechnologyforvascularsmoothmusclecellsablation AT antoniivorra nonthermalirreversibleelectroporationnoveltechnologyforvascularsmoothmusclecellsablation AT borisrubinsky nonthermalirreversibleelectroporationnoveltechnologyforvascularsmoothmusclecellsablation
_version_	1718414006935355392

Non thermal irreversible electroporation: novel technology for vascular smooth muscle cells ablation.

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