Tight Junction barriers in human hair follicles – role of claudin-1

Abstract Barrier function of hair follicles (HFs) is of great interest because they might be an entry port for allergens/pathogens, but could on the other hand be used for drug delivery or vaccination. Therefore we investigated tight junction (TJ) barrier function in human HFs. We show that there is...

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Autores principales:	Michaela Zorn-Kruppa, Sabine Vidal-y-Sy, Pia Houdek, Ewa Wladykowski, Stephan Grzybowski, Robert Gruber, Christian Gorzelanny, Jason Harcup, Stefan W. Schneider, Amitabha Majumdar, Johanna M. Brandner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc5fbde3bcd7440382fb6bdc70936aba
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spelling	oai:doaj.org-article:bc5fbde3bcd7440382fb6bdc70936aba2021-12-02T15:09:10ZTight Junction barriers in human hair follicles – role of claudin-110.1038/s41598-018-30341-92045-2322https://doaj.org/article/bc5fbde3bcd7440382fb6bdc70936aba2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30341-9https://doaj.org/toc/2045-2322Abstract Barrier function of hair follicles (HFs) is of great interest because they might be an entry port for allergens/pathogens, but could on the other hand be used for drug delivery or vaccination. Therefore we investigated tight junction (TJ) barrier function in human HFs. We show that there is a TJ barrier in the outermost living layer bordering to the environment from the infundibulum to the lower central part and between Henle’s and Huxles layer of anagen HFs. In club hair typical for catagen and telogen HFs a TJ barrier is found surrounding the club. This demonstrates that there is a continuous TJ barrier along interfollicular epidermis and HFs in different phases of HF cycle. However, interestingly, in cell culture experiments we can show that barrier is less tight in HF keratinocytes compared to interfollicular keratinocytes. Knock-down of the TJ protein claudin-1, which we demonstrate here to be less expressed in HFs of lesional atopic dermatitis skin, results in impaired barrier function, decreased proliferation and increased apoptosis of hair keratinocytes. This is in line with a hair growth phenotype in claudin-1 deficient patients (NISCH syndrome) and corresponding knock-out mice and indicates an important role of claudin-1 in HF barrier function and growth.Michaela Zorn-KruppaSabine Vidal-y-SyPia HoudekEwa WladykowskiStephan GrzybowskiRobert GruberChristian GorzelannyJason HarcupStefan W. SchneiderAmitabha MajumdarJohanna M. BrandnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michaela Zorn-Kruppa Sabine Vidal-y-Sy Pia Houdek Ewa Wladykowski Stephan Grzybowski Robert Gruber Christian Gorzelanny Jason Harcup Stefan W. Schneider Amitabha Majumdar Johanna M. Brandner Tight Junction barriers in human hair follicles – role of claudin-1
description	Abstract Barrier function of hair follicles (HFs) is of great interest because they might be an entry port for allergens/pathogens, but could on the other hand be used for drug delivery or vaccination. Therefore we investigated tight junction (TJ) barrier function in human HFs. We show that there is a TJ barrier in the outermost living layer bordering to the environment from the infundibulum to the lower central part and between Henle’s and Huxles layer of anagen HFs. In club hair typical for catagen and telogen HFs a TJ barrier is found surrounding the club. This demonstrates that there is a continuous TJ barrier along interfollicular epidermis and HFs in different phases of HF cycle. However, interestingly, in cell culture experiments we can show that barrier is less tight in HF keratinocytes compared to interfollicular keratinocytes. Knock-down of the TJ protein claudin-1, which we demonstrate here to be less expressed in HFs of lesional atopic dermatitis skin, results in impaired barrier function, decreased proliferation and increased apoptosis of hair keratinocytes. This is in line with a hair growth phenotype in claudin-1 deficient patients (NISCH syndrome) and corresponding knock-out mice and indicates an important role of claudin-1 in HF barrier function and growth.
format	article
author	Michaela Zorn-Kruppa Sabine Vidal-y-Sy Pia Houdek Ewa Wladykowski Stephan Grzybowski Robert Gruber Christian Gorzelanny Jason Harcup Stefan W. Schneider Amitabha Majumdar Johanna M. Brandner
author_facet	Michaela Zorn-Kruppa Sabine Vidal-y-Sy Pia Houdek Ewa Wladykowski Stephan Grzybowski Robert Gruber Christian Gorzelanny Jason Harcup Stefan W. Schneider Amitabha Majumdar Johanna M. Brandner
author_sort	Michaela Zorn-Kruppa
title	Tight Junction barriers in human hair follicles – role of claudin-1
title_short	Tight Junction barriers in human hair follicles – role of claudin-1
title_full	Tight Junction barriers in human hair follicles – role of claudin-1
title_fullStr	Tight Junction barriers in human hair follicles – role of claudin-1
title_full_unstemmed	Tight Junction barriers in human hair follicles – role of claudin-1
title_sort	tight junction barriers in human hair follicles – role of claudin-1
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/bc5fbde3bcd7440382fb6bdc70936aba
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Tight Junction barriers in human hair follicles – role of claudin-1

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