Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia

Abstract Persistent HPV infection associated with immune modulation may result in high-grade squamous intraepithelial lesions (CIN)2/3. Currently, there is little information on the cervicovaginal microbiome, local cytokine levels and HPV infection related to CIN. Follow-up of patients after local s...

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Autores principales:	Rina Kawahara, Takuma Fujii, Iwao Kukimoto, Hiroyuki Nomura, Rie Kawasaki, Eiji Nishio, Ryoko Ichikawa, Tetsuya Tsukamoto, Aya Iwata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc6024f673e74844b560dd2209d1aa59
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spelling	oai:doaj.org-article:bc6024f673e74844b560dd2209d1aa592021-12-02T13:24:26ZChanges to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia10.1038/s41598-020-80176-62045-2322https://doaj.org/article/bc6024f673e74844b560dd2209d1aa592021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80176-6https://doaj.org/toc/2045-2322Abstract Persistent HPV infection associated with immune modulation may result in high-grade squamous intraepithelial lesions (CIN)2/3. Currently, there is little information on the cervicovaginal microbiome, local cytokine levels and HPV infection related to CIN. Follow-up of patients after local surgery provides an opportunity to monitor changes in the cervicovaginal environment. Accordingly, we undertook this longitudinal retrospective study to determine associations between HPV genotypes, cervicovaginal microbiome and local cytokine profiles in 41 Japanese patients with CIN. Cervicovaginal microbiota were identified using universal 16S rRNA gene (rDNA) bacterial primers for the V3/4 region by PCR of genomic DNA, followed by MiSeq sequencing. We found that Atopobium vaginae was significantly decreased (p < 0.047), whereas A. ureaplasma (p < 0.022) increased after surgery. Cytokine levels in cervical mucus were measured by multiplexed bead-based immunoassays, revealing that IL-1β (p < 0.006), TNF-α (p < 0.004), MIP-1α (p < 0.045) and eotaxin (p < 0.003) were significantly decreased after surgery. Notably, the level of eotaxin decreased in parallel with HPV clearance after surgery (p < 0.028). Thus, local surgery affected the cervicovaginal microbiome, status of HPV infection and immune response. Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia may be important for understanding the pathogenesis of CIN in future.Rina KawaharaTakuma FujiiIwao KukimotoHiroyuki NomuraRie KawasakiEiji NishioRyoko IchikawaTetsuya TsukamotoAya IwataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rina Kawahara Takuma Fujii Iwao Kukimoto Hiroyuki Nomura Rie Kawasaki Eiji Nishio Ryoko Ichikawa Tetsuya Tsukamoto Aya Iwata Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
description	Abstract Persistent HPV infection associated with immune modulation may result in high-grade squamous intraepithelial lesions (CIN)2/3. Currently, there is little information on the cervicovaginal microbiome, local cytokine levels and HPV infection related to CIN. Follow-up of patients after local surgery provides an opportunity to monitor changes in the cervicovaginal environment. Accordingly, we undertook this longitudinal retrospective study to determine associations between HPV genotypes, cervicovaginal microbiome and local cytokine profiles in 41 Japanese patients with CIN. Cervicovaginal microbiota were identified using universal 16S rRNA gene (rDNA) bacterial primers for the V3/4 region by PCR of genomic DNA, followed by MiSeq sequencing. We found that Atopobium vaginae was significantly decreased (p < 0.047), whereas A. ureaplasma (p < 0.022) increased after surgery. Cytokine levels in cervical mucus were measured by multiplexed bead-based immunoassays, revealing that IL-1β (p < 0.006), TNF-α (p < 0.004), MIP-1α (p < 0.045) and eotaxin (p < 0.003) were significantly decreased after surgery. Notably, the level of eotaxin decreased in parallel with HPV clearance after surgery (p < 0.028). Thus, local surgery affected the cervicovaginal microbiome, status of HPV infection and immune response. Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia may be important for understanding the pathogenesis of CIN in future.
format	article
author	Rina Kawahara Takuma Fujii Iwao Kukimoto Hiroyuki Nomura Rie Kawasaki Eiji Nishio Ryoko Ichikawa Tetsuya Tsukamoto Aya Iwata
author_facet	Rina Kawahara Takuma Fujii Iwao Kukimoto Hiroyuki Nomura Rie Kawasaki Eiji Nishio Ryoko Ichikawa Tetsuya Tsukamoto Aya Iwata
author_sort	Rina Kawahara
title	Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
title_short	Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
title_full	Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
title_fullStr	Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
title_full_unstemmed	Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
title_sort	changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bc6024f673e74844b560dd2209d1aa59
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Changes to the cervicovaginal microbiota and cervical cytokine profile following surgery for cervical intraepithelial neoplasia

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