Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice

Abstract Obesity and dyslipidemia are hallmarks of metabolic and cardiovascular diseases. Polydextrose (PDX), a soluble fiber has lipid lowering effects. We hypothesize that PDX reduces triglycerides and cholesterol by influencing gut microbiota, which in turn modulate intestinal gene expression. C5...

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Autores principales:	Ghulam Shere Raza, Heli Putaala, Ashley A. Hibberd, Esa Alhoniemi, Kirsti Tiihonen, Kari Antero Mäkelä, Karl-Heinz Herzig
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc618b4fd25942e8acb8aeba2f0a6a61
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spelling	oai:doaj.org-article:bc618b4fd25942e8acb8aeba2f0a6a612021-12-02T11:52:37ZPolydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice10.1038/s41598-017-05259-32045-2322https://doaj.org/article/bc618b4fd25942e8acb8aeba2f0a6a612017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05259-3https://doaj.org/toc/2045-2322Abstract Obesity and dyslipidemia are hallmarks of metabolic and cardiovascular diseases. Polydextrose (PDX), a soluble fiber has lipid lowering effects. We hypothesize that PDX reduces triglycerides and cholesterol by influencing gut microbiota, which in turn modulate intestinal gene expression. C57BL/6 male mice were fed a Western diet (WD) ±75 mg PDX twice daily by oral gavage for 14 days. Body weight and food intake were monitored daily. Fasting plasma lipids, caecal microbiota and gene expression in intestine and liver were measured after 14 days of feeding. PDX supplementation to WD significantly reduced food intake (p < 0.001), fasting plasma triglyceride (p < 0.001) and total cholesterol (p < 0.05). Microbiome analysis revealed that the relative abundance of Allobaculum, Bifidobacterium and Coriobacteriaceae taxa associated with lean phenotype, increased in WD + PDX mice. Gene expression analysis with linear mixed-effects model showed consistent downregulation of Dgat1, Cd36, Fiaf and upregulation of Fxr in duodenum, jejunum, ileum and colon in WD + PDX mice. Spearman correlations indicated that genera enriched in WD + PDX mice inversely correlated with fasting lipids and downregulated genes Dgat1, Cd36 and Fiaf while positively with upregulated gene Fxr. These results suggest that PDX in mice fed WD promoted systemic changes via regulation of the gut microbiota and gene expression in intestinal tract.Ghulam Shere RazaHeli PutaalaAshley A. HibberdEsa AlhoniemiKirsti TiihonenKari Antero MäkeläKarl-Heinz HerzigNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ghulam Shere Raza Heli Putaala Ashley A. Hibberd Esa Alhoniemi Kirsti Tiihonen Kari Antero Mäkelä Karl-Heinz Herzig Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice
description	Abstract Obesity and dyslipidemia are hallmarks of metabolic and cardiovascular diseases. Polydextrose (PDX), a soluble fiber has lipid lowering effects. We hypothesize that PDX reduces triglycerides and cholesterol by influencing gut microbiota, which in turn modulate intestinal gene expression. C57BL/6 male mice were fed a Western diet (WD) ±75 mg PDX twice daily by oral gavage for 14 days. Body weight and food intake were monitored daily. Fasting plasma lipids, caecal microbiota and gene expression in intestine and liver were measured after 14 days of feeding. PDX supplementation to WD significantly reduced food intake (p < 0.001), fasting plasma triglyceride (p < 0.001) and total cholesterol (p < 0.05). Microbiome analysis revealed that the relative abundance of Allobaculum, Bifidobacterium and Coriobacteriaceae taxa associated with lean phenotype, increased in WD + PDX mice. Gene expression analysis with linear mixed-effects model showed consistent downregulation of Dgat1, Cd36, Fiaf and upregulation of Fxr in duodenum, jejunum, ileum and colon in WD + PDX mice. Spearman correlations indicated that genera enriched in WD + PDX mice inversely correlated with fasting lipids and downregulated genes Dgat1, Cd36 and Fiaf while positively with upregulated gene Fxr. These results suggest that PDX in mice fed WD promoted systemic changes via regulation of the gut microbiota and gene expression in intestinal tract.
format	article
author	Ghulam Shere Raza Heli Putaala Ashley A. Hibberd Esa Alhoniemi Kirsti Tiihonen Kari Antero Mäkelä Karl-Heinz Herzig
author_facet	Ghulam Shere Raza Heli Putaala Ashley A. Hibberd Esa Alhoniemi Kirsti Tiihonen Kari Antero Mäkelä Karl-Heinz Herzig
author_sort	Ghulam Shere Raza
title	Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice
title_short	Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice
title_full	Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice
title_fullStr	Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice
title_full_unstemmed	Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice
title_sort	polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in western diet fed mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc618b4fd25942e8acb8aeba2f0a6a61
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Polydextrose changes the gut microbiome and attenuates fasting triglyceride and cholesterol levels in Western diet fed mice

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