Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

Christine G, Budiarti S, Astuti RI. 2018. Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach. Biodiversitas 19: 1375-1381. Urinary tract infection (UTI) is a common bacterial infection in childhood in both males and females. The infection is usually...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	GRACE CHRISTINE, SRI BUDIARTI, INDRI ASTUTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	bacteria dgge uti urine 16s rrna Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bc6c691d905e4dc3b336e5e2109be0c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bc6c691d905e4dc3b336e5e2109be0c3
record_format	dspace
spelling	oai:doaj.org-article:bc6c691d905e4dc3b336e5e2109be0c32021-11-16T14:03:29ZDiversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach1412-033X2085-472210.13057/biodiv/d190425https://doaj.org/article/bc6c691d905e4dc3b336e5e2109be0c32018-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2795https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Christine G, Budiarti S, Astuti RI. 2018. Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach. Biodiversitas 19: 1375-1381. Urinary tract infection (UTI) is a common bacterial infection in childhood in both males and females. The infection is usually caused by bacterial invasion of the urinary tract including the lower and the upper urinary tract. In Indonesia, to the best our knowledge, the diversity of urinary tract infection bacteria has not been reported yet. Therefore, the aims of this study were to identify the diversity of both culturable and unculturable bacteria in children diagnosed with UTI. In this study, urine samples were obtained from different age groups ranging 6-17 years. Analysis of 16S rRNA gene sequence showed eight culturable isolates (SBU1, SBU2, SBU3, SBU4, SBU5, SBU6, SBU7, SBU8) are closely related to Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, Citrobacter sp. and Acinetobacter sp. with maximum identity up to 98-99%. Diversity of unculturable bacteria community based on 16S rRNA gene was represented by 9 DGGE (Denaturing Gradient Gel Electrophoresis) bands. The nine respective bands showed the similarity ranging from 84 up to 96% with Klebsiella sp, Escherichia sp., Lactococcus lactic, Shigella flexneri and uncultured bacterium. Based on phylogenetic analysis, all culturable isolates belong to phylum Proteobacteria, which is dominated by family of Enterobacteriaceae. Interestingly, by using metagenomic approach, it is observed that bacteria belong to phylum Firmicutes were found in the UTI-diagnosed patients, in addition to those bacterial isolates from phylum Proteobacteria. To our knowledge, this is the first study to report the occurrence of Firmicutes and Proteobacteria in UTI-diagnosed patients in Indonesia.GRACE CHRISTINESRI BUDIARTIINDRI ASTUTIMBI & UNS Soloarticlebacteriadggeutiurine16s rrnaBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 4, Pp 1375-1381 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria dgge uti urine 16s rrna Biology (General) QH301-705.5
spellingShingle	bacteria dgge uti urine 16s rrna Biology (General) QH301-705.5 GRACE CHRISTINE SRI BUDIARTI INDRI ASTUTI Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach
description	Christine G, Budiarti S, Astuti RI. 2018. Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach. Biodiversitas 19: 1375-1381. Urinary tract infection (UTI) is a common bacterial infection in childhood in both males and females. The infection is usually caused by bacterial invasion of the urinary tract including the lower and the upper urinary tract. In Indonesia, to the best our knowledge, the diversity of urinary tract infection bacteria has not been reported yet. Therefore, the aims of this study were to identify the diversity of both culturable and unculturable bacteria in children diagnosed with UTI. In this study, urine samples were obtained from different age groups ranging 6-17 years. Analysis of 16S rRNA gene sequence showed eight culturable isolates (SBU1, SBU2, SBU3, SBU4, SBU5, SBU6, SBU7, SBU8) are closely related to Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, Citrobacter sp. and Acinetobacter sp. with maximum identity up to 98-99%. Diversity of unculturable bacteria community based on 16S rRNA gene was represented by 9 DGGE (Denaturing Gradient Gel Electrophoresis) bands. The nine respective bands showed the similarity ranging from 84 up to 96% with Klebsiella sp, Escherichia sp., Lactococcus lactic, Shigella flexneri and uncultured bacterium. Based on phylogenetic analysis, all culturable isolates belong to phylum Proteobacteria, which is dominated by family of Enterobacteriaceae. Interestingly, by using metagenomic approach, it is observed that bacteria belong to phylum Firmicutes were found in the UTI-diagnosed patients, in addition to those bacterial isolates from phylum Proteobacteria. To our knowledge, this is the first study to report the occurrence of Firmicutes and Proteobacteria in UTI-diagnosed patients in Indonesia.
format	article
author	GRACE CHRISTINE SRI BUDIARTI INDRI ASTUTI
author_facet	GRACE CHRISTINE SRI BUDIARTI INDRI ASTUTI
author_sort	GRACE CHRISTINE
title	Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach
title_short	Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach
title_full	Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach
title_fullStr	Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach
title_full_unstemmed	Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach
title_sort	diversity of urinary tract infection bacteria in children in indonesia based on metagenomic approach
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/bc6c691d905e4dc3b336e5e2109be0c3
work_keys_str_mv	AT gracechristine diversityofurinarytractinfectionbacteriainchildreninindonesiabasedonmetagenomicapproach AT sribudiarti diversityofurinarytractinfectionbacteriainchildreninindonesiabasedonmetagenomicapproach AT indriastuti diversityofurinarytractinfectionbacteriainchildreninindonesiabasedonmetagenomicapproach
_version_	1718426445436420096

Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

Ejemplares similares