A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury

The widespread use of fluoroquinolones (FQs) causes a serious risk to the environment and human health. Here, we evaluated the potential effect to induce testis damage by gatifloxacin (GAT) intragastrically treatment in mice (25, 50, and 100 mg/kg body weight per day for 7 days). We observed testicu...

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Autores principales:	Ruixian Guo, Junping Lv, Huibo Xu, Yinghui Bai, Binan Lu, Ying Han
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Gatifloxacin RNA sequencing Transcriptomics Testicular toxicity Spermatogenesis Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/bc6f7e7ecee243a7a67975c0386ad8eb
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spelling	oai:doaj.org-article:bc6f7e7ecee243a7a67975c0386ad8eb2021-11-20T04:55:39ZA systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury0147-651310.1016/j.ecoenv.2021.113002https://doaj.org/article/bc6f7e7ecee243a7a67975c0386ad8eb2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011143https://doaj.org/toc/0147-6513The widespread use of fluoroquinolones (FQs) causes a serious risk to the environment and human health. Here, we evaluated the potential effect to induce testis damage by gatifloxacin (GAT) intragastrically treatment in mice (25, 50, and 100 mg/kg body weight per day for 7 days). We observed testicular weight, serum testosterone, antioxidant enzyme activity, and mRNA levels and pathways. Testicular histopathology indicated that GAT administration induced a dose-dependent spermatogenesis abnormality. At 50 mg/kg, GAT altered gene expression but did not change the weight and the levels of testosterone and antioxidant enzymes. These findings indicate that mRNA levels are more sensitive than weight and testosterone for detecting GAT testicular toxicity. We also found that GAT induced testicular damage by regulating the candidate genes associated with spermatogenesis, germ cell movement, testicular fibrosis, and reproductive axis development. This study enhances our perception of the mechanism of FQs-induced testicular toxicity and environmental effects. However, the molecular mechanism needs to be further researched.Ruixian GuoJunping LvHuibo XuYinghui BaiBinan LuYing HanElsevierarticleGatifloxacinRNA sequencingTranscriptomicsTesticular toxicitySpermatogenesisEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 113002- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gatifloxacin RNA sequencing Transcriptomics Testicular toxicity Spermatogenesis Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Gatifloxacin RNA sequencing Transcriptomics Testicular toxicity Spermatogenesis Environmental pollution TD172-193.5 Environmental sciences GE1-350 Ruixian Guo Junping Lv Huibo Xu Yinghui Bai Binan Lu Ying Han A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
description	The widespread use of fluoroquinolones (FQs) causes a serious risk to the environment and human health. Here, we evaluated the potential effect to induce testis damage by gatifloxacin (GAT) intragastrically treatment in mice (25, 50, and 100 mg/kg body weight per day for 7 days). We observed testicular weight, serum testosterone, antioxidant enzyme activity, and mRNA levels and pathways. Testicular histopathology indicated that GAT administration induced a dose-dependent spermatogenesis abnormality. At 50 mg/kg, GAT altered gene expression but did not change the weight and the levels of testosterone and antioxidant enzymes. These findings indicate that mRNA levels are more sensitive than weight and testosterone for detecting GAT testicular toxicity. We also found that GAT induced testicular damage by regulating the candidate genes associated with spermatogenesis, germ cell movement, testicular fibrosis, and reproductive axis development. This study enhances our perception of the mechanism of FQs-induced testicular toxicity and environmental effects. However, the molecular mechanism needs to be further researched.
format	article
author	Ruixian Guo Junping Lv Huibo Xu Yinghui Bai Binan Lu Ying Han
author_facet	Ruixian Guo Junping Lv Huibo Xu Yinghui Bai Binan Lu Ying Han
author_sort	Ruixian Guo
title	A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
title_short	A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
title_full	A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
title_fullStr	A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
title_full_unstemmed	A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
title_sort	systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/bc6f7e7ecee243a7a67975c0386ad8eb
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A systems toxicology approach to explore toxicological mechanisms of fluoroquinolones-induced testis injury

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