Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study

Introduction Polymorphisms in genes coding enzymes involved in folate metabolism may cause alterations in this metabolic pathway and contribute to carcinogenesis, because folate is essential for DNA synthesis, methylation and repair. The objective of this study was to investigate the association of...

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Autores principales:	Tairine Zara-Lopes, Ana Lívia Silva Galbiatti-Dias, Márcia Maria Urbanin Castanhole-Nunes, João Armando Padovani-Júnior, José Victor Maniglia, Erika Cristina Pavarino, Eny Maria Goloni-Bertollo
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	genetic polymorphism genes thyroid cancer Medicine R
Acceso en línea:	https://doaj.org/article/bc715fc24f1242be8a944351af26f569
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spelling	oai:doaj.org-article:bc715fc24f1242be8a944351af26f5692021-12-02T18:39:10ZPolymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study1734-19221896-915110.5114/aoms.2018.73091https://doaj.org/article/bc715fc24f1242be8a944351af26f5692019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Polymorphisms-in-MTHFR-MTR-RFC1-and-CssS-genes-involved-in-folate-metabolism-and,70468,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Polymorphisms in genes coding enzymes involved in folate metabolism may cause alterations in this metabolic pathway and contribute to carcinogenesis, because folate is essential for DNA synthesis, methylation and repair. The objective of this study was to investigate the association of MTHFR 677C>T (rs1801133), MTR 2756A>G (rs1805087), RFC1 80A>G (rs1051266) and CßS 844ins(68) (no rs#) polymorphisms and thyroid cancer development. The association of these polymorphisms with demographic risk factors and clinical histopathological parameters was also evaluated. Material and methods The study is a case-control analysis with a total of 462 individuals (151 patients and 311 controls). Polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping. The 2 and multiple logistic regression were utilized for statistical analysis. Results The polymorphisms analysis revealed an association between the MTHFR 677C>T polymorphism (OR = 2.87, 95% CI: 1.50–5.48, p < 0.01, codominant model), (OR = 1.76, 95% CI: 1.18–2.64, p < 0.01, dominant model), (OR = 2.37, 95% CI: 1.28–4.39, p G polymorphism also was associated with thyroid cancer under recessive mode of inheritance (OR = 1.55; 95% CI: 1.02–2.38; p = 0.04); however, this polymorphism showed Hardy-Weinberg disequilibrium in the control group (χ 2 = 24.71, p < 0.001). Furthermore, alcohol (OR = 1.56, 95% CI: 1.36–1.89, p < 0.01) and tobacco consumption (OR = 1.97, 95% CI: 1.28–3.04, p G polymorphism showed an association with tumor extent (OR = 2.69, 95% CI: 1.27–5.71, p < 0.01) and aggressiveness (OR = 4.51, 95% CI: 1.67–12.1, p T is significantly associated with increased risk for thyroid cancer and MTR 2756A>G is associated with tumor extent and aggressiveness. In addition, alcohol and tobacco consumption were associated with increased risk of thyroid cancer. These results may contribute to a better prognosis for thyroid cancer.Tairine Zara-LopesAna Lívia Silva Galbiatti-DiasMárcia Maria Urbanin Castanhole-NunesJoão Armando Padovani-JúniorJosé Victor ManigliaErika Cristina PavarinoEny Maria Goloni-BertolloTermedia Publishing Housearticlegenetic polymorphismgenesthyroid cancergenesMedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 522-530 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic polymorphism genes thyroid cancer genes Medicine R
spellingShingle	genetic polymorphism genes thyroid cancer genes Medicine R Tairine Zara-Lopes Ana Lívia Silva Galbiatti-Dias Márcia Maria Urbanin Castanhole-Nunes João Armando Padovani-Júnior José Victor Maniglia Erika Cristina Pavarino Eny Maria Goloni-Bertollo Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
description	Introduction Polymorphisms in genes coding enzymes involved in folate metabolism may cause alterations in this metabolic pathway and contribute to carcinogenesis, because folate is essential for DNA synthesis, methylation and repair. The objective of this study was to investigate the association of MTHFR 677C>T (rs1801133), MTR 2756A>G (rs1805087), RFC1 80A>G (rs1051266) and CßS 844ins(68) (no rs#) polymorphisms and thyroid cancer development. The association of these polymorphisms with demographic risk factors and clinical histopathological parameters was also evaluated. Material and methods The study is a case-control analysis with a total of 462 individuals (151 patients and 311 controls). Polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping. The 2 and multiple logistic regression were utilized for statistical analysis. Results The polymorphisms analysis revealed an association between the MTHFR 677C>T polymorphism (OR = 2.87, 95% CI: 1.50–5.48, p < 0.01, codominant model), (OR = 1.76, 95% CI: 1.18–2.64, p < 0.01, dominant model), (OR = 2.37, 95% CI: 1.28–4.39, p G polymorphism also was associated with thyroid cancer under recessive mode of inheritance (OR = 1.55; 95% CI: 1.02–2.38; p = 0.04); however, this polymorphism showed Hardy-Weinberg disequilibrium in the control group (χ 2 = 24.71, p < 0.001). Furthermore, alcohol (OR = 1.56, 95% CI: 1.36–1.89, p < 0.01) and tobacco consumption (OR = 1.97, 95% CI: 1.28–3.04, p G polymorphism showed an association with tumor extent (OR = 2.69, 95% CI: 1.27–5.71, p < 0.01) and aggressiveness (OR = 4.51, 95% CI: 1.67–12.1, p T is significantly associated with increased risk for thyroid cancer and MTR 2756A>G is associated with tumor extent and aggressiveness. In addition, alcohol and tobacco consumption were associated with increased risk of thyroid cancer. These results may contribute to a better prognosis for thyroid cancer.
format	article
author	Tairine Zara-Lopes Ana Lívia Silva Galbiatti-Dias Márcia Maria Urbanin Castanhole-Nunes João Armando Padovani-Júnior José Victor Maniglia Erika Cristina Pavarino Eny Maria Goloni-Bertollo
author_facet	Tairine Zara-Lopes Ana Lívia Silva Galbiatti-Dias Márcia Maria Urbanin Castanhole-Nunes João Armando Padovani-Júnior José Victor Maniglia Erika Cristina Pavarino Eny Maria Goloni-Bertollo
author_sort	Tairine Zara-Lopes
title	Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
title_short	Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
title_full	Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
title_fullStr	Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
title_full_unstemmed	Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
title_sort	polymorphisms in mthfr, mtr, rfc1 and cßs genes involved in folate metabolism and thyroid cancer: a case-control study
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/bc715fc24f1242be8a944351af26f569
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Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study

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