Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis

Abstract Infectious pancreatic necrosis virus (IPNV) is a non-enveloped virus belonging to the Birnaviridae family. IPNV produces an acute disease in salmon fingerlings, with high mortality rates and persistent infection in survivors. Although there are reports of IPNV binding to various cells, the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jorge Levican, Camila Miranda-Cárdenas, Ricardo Soto-Rifo, Francisco Aguayo, Aldo Gaggero, Oscar León
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc754f3df4a145ef919c9bb90f9326ba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bc754f3df4a145ef919c9bb90f9326ba
record_format	dspace
spelling	oai:doaj.org-article:bc754f3df4a145ef919c9bb90f9326ba2021-12-02T15:05:09ZInfectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis10.1038/s41598-017-03036-w2045-2322https://doaj.org/article/bc754f3df4a145ef919c9bb90f9326ba2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03036-whttps://doaj.org/toc/2045-2322Abstract Infectious pancreatic necrosis virus (IPNV) is a non-enveloped virus belonging to the Birnaviridae family. IPNV produces an acute disease in salmon fingerlings, with high mortality rates and persistent infection in survivors. Although there are reports of IPNV binding to various cells, the viral receptor and entry pathways remain unknown. The aim of this study was to determine the endocytic pathway that allows for IPNV entry. We observed that IPNV stimulated fluid uptake and virus particles co-localysed with the uptake marker dextran in intracellular compartments, suggesting a role for macropinocytosis in viral entry. Consistent with this idea, viral infection was significantly reduced when the Na+/H+ exchanger NHE1 was inhibited with 5-(N-Ethyl-N-isopropyl) amiloride (EIPA). Neither chlorpromazine nor filipin complex I affected IPNV infection. To examine the role of macropinocytosis regulators, additional inhibitors were tested. Inhibitors of the EGFR pathway and the effectors Pak1, Rac1 and PKC reduced viral infection. Together, our results indicate that IPNV is mainly internalized into CHSE-214 cells by macropinocytosis.Jorge LevicanCamila Miranda-CárdenasRicardo Soto-RifoFrancisco AguayoAldo GaggeroOscar LeónNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jorge Levican Camila Miranda-Cárdenas Ricardo Soto-Rifo Francisco Aguayo Aldo Gaggero Oscar León Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
description	Abstract Infectious pancreatic necrosis virus (IPNV) is a non-enveloped virus belonging to the Birnaviridae family. IPNV produces an acute disease in salmon fingerlings, with high mortality rates and persistent infection in survivors. Although there are reports of IPNV binding to various cells, the viral receptor and entry pathways remain unknown. The aim of this study was to determine the endocytic pathway that allows for IPNV entry. We observed that IPNV stimulated fluid uptake and virus particles co-localysed with the uptake marker dextran in intracellular compartments, suggesting a role for macropinocytosis in viral entry. Consistent with this idea, viral infection was significantly reduced when the Na+/H+ exchanger NHE1 was inhibited with 5-(N-Ethyl-N-isopropyl) amiloride (EIPA). Neither chlorpromazine nor filipin complex I affected IPNV infection. To examine the role of macropinocytosis regulators, additional inhibitors were tested. Inhibitors of the EGFR pathway and the effectors Pak1, Rac1 and PKC reduced viral infection. Together, our results indicate that IPNV is mainly internalized into CHSE-214 cells by macropinocytosis.
format	article
author	Jorge Levican Camila Miranda-Cárdenas Ricardo Soto-Rifo Francisco Aguayo Aldo Gaggero Oscar León
author_facet	Jorge Levican Camila Miranda-Cárdenas Ricardo Soto-Rifo Francisco Aguayo Aldo Gaggero Oscar León
author_sort	Jorge Levican
title	Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_short	Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_full	Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_fullStr	Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_full_unstemmed	Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_sort	infectious pancreatic necrosis virus enters chse-214 cells via macropinocytosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc754f3df4a145ef919c9bb90f9326ba
work_keys_str_mv	AT jorgelevican infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis AT camilamirandacardenas infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis AT ricardosotorifo infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis AT franciscoaguayo infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis AT aldogaggero infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis AT oscarleon infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
_version_	1718388918947151872

Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis

Ejemplares similares