Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis

Abstract Infectious pancreatic necrosis virus (IPNV) is a non-enveloped virus belonging to the Birnaviridae family. IPNV produces an acute disease in salmon fingerlings, with high mortality rates and persistent infection in survivors. Although there are reports of IPNV binding to various cells, the...

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Autores principales: Jorge Levican, Camila Miranda-Cárdenas, Ricardo Soto-Rifo, Francisco Aguayo, Aldo Gaggero, Oscar León
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/bc754f3df4a145ef919c9bb90f9326ba
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spelling oai:doaj.org-article:bc754f3df4a145ef919c9bb90f9326ba2021-12-02T15:05:09ZInfectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis10.1038/s41598-017-03036-w2045-2322https://doaj.org/article/bc754f3df4a145ef919c9bb90f9326ba2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03036-whttps://doaj.org/toc/2045-2322Abstract Infectious pancreatic necrosis virus (IPNV) is a non-enveloped virus belonging to the Birnaviridae family. IPNV produces an acute disease in salmon fingerlings, with high mortality rates and persistent infection in survivors. Although there are reports of IPNV binding to various cells, the viral receptor and entry pathways remain unknown. The aim of this study was to determine the endocytic pathway that allows for IPNV entry. We observed that IPNV stimulated fluid uptake and virus particles co-localysed with the uptake marker dextran in intracellular compartments, suggesting a role for macropinocytosis in viral entry. Consistent with this idea, viral infection was significantly reduced when the Na+/H+ exchanger NHE1 was inhibited with 5-(N-Ethyl-N-isopropyl) amiloride (EIPA). Neither chlorpromazine nor filipin complex I affected IPNV infection. To examine the role of macropinocytosis regulators, additional inhibitors were tested. Inhibitors of the EGFR pathway and the effectors Pak1, Rac1 and PKC reduced viral infection. Together, our results indicate that IPNV is mainly internalized into CHSE-214 cells by macropinocytosis.Jorge LevicanCamila Miranda-CárdenasRicardo Soto-RifoFrancisco AguayoAldo GaggeroOscar LeónNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jorge Levican
Camila Miranda-Cárdenas
Ricardo Soto-Rifo
Francisco Aguayo
Aldo Gaggero
Oscar León
Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
description Abstract Infectious pancreatic necrosis virus (IPNV) is a non-enveloped virus belonging to the Birnaviridae family. IPNV produces an acute disease in salmon fingerlings, with high mortality rates and persistent infection in survivors. Although there are reports of IPNV binding to various cells, the viral receptor and entry pathways remain unknown. The aim of this study was to determine the endocytic pathway that allows for IPNV entry. We observed that IPNV stimulated fluid uptake and virus particles co-localysed with the uptake marker dextran in intracellular compartments, suggesting a role for macropinocytosis in viral entry. Consistent with this idea, viral infection was significantly reduced when the Na+/H+ exchanger NHE1 was inhibited with 5-(N-Ethyl-N-isopropyl) amiloride (EIPA). Neither chlorpromazine nor filipin complex I affected IPNV infection. To examine the role of macropinocytosis regulators, additional inhibitors were tested. Inhibitors of the EGFR pathway and the effectors Pak1, Rac1 and PKC reduced viral infection. Together, our results indicate that IPNV is mainly internalized into CHSE-214 cells by macropinocytosis.
format article
author Jorge Levican
Camila Miranda-Cárdenas
Ricardo Soto-Rifo
Francisco Aguayo
Aldo Gaggero
Oscar León
author_facet Jorge Levican
Camila Miranda-Cárdenas
Ricardo Soto-Rifo
Francisco Aguayo
Aldo Gaggero
Oscar León
author_sort Jorge Levican
title Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_short Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_full Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_fullStr Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_full_unstemmed Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis
title_sort infectious pancreatic necrosis virus enters chse-214 cells via macropinocytosis
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/bc754f3df4a145ef919c9bb90f9326ba
work_keys_str_mv AT jorgelevican infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
AT camilamirandacardenas infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
AT ricardosotorifo infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
AT franciscoaguayo infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
AT aldogaggero infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
AT oscarleon infectiouspancreaticnecrosisvirusenterschse214cellsviamacropinocytosis
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