The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.

Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a,...

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Autores principales:	Renata Viesti A Collares, Wilson Salgado, Daniela Pretti da Cunha Tirapelli, José Sebastião dos Santos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc7831c5e5084aea89dc3df7a09d7bcd
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spelling	oai:doaj.org-article:bc7831c5e5084aea89dc3df7a09d7bcd2021-11-18T08:25:33ZThe expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.1932-620310.1371/journal.pone.0093512https://doaj.org/article/bc7831c5e5084aea89dc3df7a09d7bcd2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24690978/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA was extracted from biopsies of subcutaneous fat, liver and visceral fat of 15 obese subjects submitted to bariatric surgery and of 15 non-obese subjects submitted to cholecystectomy for cDNA synthesis and for RT-PCR. The microRNAs were chosen using the TargetScan software. An increased expression of LEP and IGF1 was detected in the subcutaneous fat of the obese group compared to control, while the expression of IGF1 was higher in the control group than in the obese one. MiRNA-27a had a higher expression in the omentum of the obese patients and there was also a correlation in the expression of miRNA-145 and LEPR in the omentum of this group.Renata Viesti A CollaresWilson SalgadoDaniela Pretti da Cunha TirapelliJosé Sebastião dos SantosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93512 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Renata Viesti A Collares Wilson Salgado Daniela Pretti da Cunha Tirapelli José Sebastião dos Santos The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.
description	Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA was extracted from biopsies of subcutaneous fat, liver and visceral fat of 15 obese subjects submitted to bariatric surgery and of 15 non-obese subjects submitted to cholecystectomy for cDNA synthesis and for RT-PCR. The microRNAs were chosen using the TargetScan software. An increased expression of LEP and IGF1 was detected in the subcutaneous fat of the obese group compared to control, while the expression of IGF1 was higher in the control group than in the obese one. MiRNA-27a had a higher expression in the omentum of the obese patients and there was also a correlation in the expression of miRNA-145 and LEPR in the omentum of this group.
format	article
author	Renata Viesti A Collares Wilson Salgado Daniela Pretti da Cunha Tirapelli José Sebastião dos Santos
author_facet	Renata Viesti A Collares Wilson Salgado Daniela Pretti da Cunha Tirapelli José Sebastião dos Santos
author_sort	Renata Viesti A Collares
title	The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.
title_short	The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.
title_full	The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.
title_fullStr	The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.
title_full_unstemmed	The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.
title_sort	expression of lep, lepr, igf1 and il10 in obesity and the relationship with micrornas.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bc7831c5e5084aea89dc3df7a09d7bcd
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The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs.

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