Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia

Abstract Neuronal and glial progenitor cells exist in the adult Drosophila brain. The primarily glial progenitor cells rely on a microRNA, mir-31a, to inhibit the expression of a predicted E3 ubiquitin ligase, CG16947. Erroneous inheritance of CG16947 by the progeny when the neural progenitor cell d...

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Autor principal:	Lynette C. Foo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc7b7d9c99f04a15949fd031d75442a8
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spelling	oai:doaj.org-article:bc7b7d9c99f04a15949fd031d75442a82021-12-02T15:05:20ZCyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia10.1038/s41598-017-07179-82045-2322https://doaj.org/article/bc7b7d9c99f04a15949fd031d75442a82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07179-8https://doaj.org/toc/2045-2322Abstract Neuronal and glial progenitor cells exist in the adult Drosophila brain. The primarily glial progenitor cells rely on a microRNA, mir-31a, to inhibit the expression of a predicted E3 ubiquitin ligase, CG16947. Erroneous inheritance of CG16947 by the progeny when the neural progenitor cell divides leads to death of the progeny, however how CG16947 achieves glial cell death is unknown. I have identified the interacting partner of CG16947 to be cdk9. I show that reduction of cdk9 expression in glia causes glial loss; highlighting the importance of cdk9 in mediating the survival of glia. Further, glial loss observed in mir-31a mutants was prevented with adult-specific expression of cdk9 in glia. I provide biochemical evidence that the binding of CG16947 to cdk9 causes its degradation. Taken together, this data shows that cdk9 plays a role in the survival of adult glia in the Drosophila brain. Thus, a fine balance exists between mir-31a and CG16947 expression in the progenitor cells that in turn regulates the levels of cdk9 in the progeny. This serves to allow the progenitor cells to regulate the number of glia in the adult brain.Lynette C. FooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lynette C. Foo Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia
description	Abstract Neuronal and glial progenitor cells exist in the adult Drosophila brain. The primarily glial progenitor cells rely on a microRNA, mir-31a, to inhibit the expression of a predicted E3 ubiquitin ligase, CG16947. Erroneous inheritance of CG16947 by the progeny when the neural progenitor cell divides leads to death of the progeny, however how CG16947 achieves glial cell death is unknown. I have identified the interacting partner of CG16947 to be cdk9. I show that reduction of cdk9 expression in glia causes glial loss; highlighting the importance of cdk9 in mediating the survival of glia. Further, glial loss observed in mir-31a mutants was prevented with adult-specific expression of cdk9 in glia. I provide biochemical evidence that the binding of CG16947 to cdk9 causes its degradation. Taken together, this data shows that cdk9 plays a role in the survival of adult glia in the Drosophila brain. Thus, a fine balance exists between mir-31a and CG16947 expression in the progenitor cells that in turn regulates the levels of cdk9 in the progeny. This serves to allow the progenitor cells to regulate the number of glia in the adult brain.
format	article
author	Lynette C. Foo
author_facet	Lynette C. Foo
author_sort	Lynette C. Foo
title	Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia
title_short	Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia
title_full	Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia
title_fullStr	Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia
title_full_unstemmed	Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia
title_sort	cyclin-dependent kinase 9 is required for the survival of adult drosophila melanogaster glia
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc7b7d9c99f04a15949fd031d75442a8
work_keys_str_mv	AT lynettecfoo cyclindependentkinase9isrequiredforthesurvivalofadultdrosophilamelanogasterglia
_version_	1718388854600237056

Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia

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