Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.

Tuberculosis is still a major health problem worldwide. Currently it is not known what kind of immune responses lead to successful control and clearance of Mycobacterium tuberculosis. This gap in knowledge is reflected by the inability to develop sufficient diagnostic and therapeutic tools to fight...

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Autores principales:	Milka Marjut Hammarén, Kaisa Ester Oksanen, Hanna Maria Nisula, Bruno Vincent Luukinen, Marko Pesu, Mika Rämet, Mataleena Parikka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bc7c0be578d64990a76b8441036111c9
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spelling	oai:doaj.org-article:bc7c0be578d64990a76b8441036111c92021-11-11T06:06:07ZAdequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.1553-73661553-737410.1371/journal.ppat.1004190https://doaj.org/article/bc7c0be578d64990a76b8441036111c92014-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24968056/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Tuberculosis is still a major health problem worldwide. Currently it is not known what kind of immune responses lead to successful control and clearance of Mycobacterium tuberculosis. This gap in knowledge is reflected by the inability to develop sufficient diagnostic and therapeutic tools to fight tuberculosis. We have used the Mycobacterium marinum infection model in the adult zebrafish and taken advantage of heterogeneity of zebrafish population to dissect the characteristics of adaptive immune responses, some of which are associated with well-controlled latency or bacterial clearance while others with progressive infection. Differences in T cell responses between subpopulations were measured at the transcriptional level. It was discovered that a high total T cell level was usually associated with lower bacterial loads alongside with a T helper 2 (Th2)-type gene expression signature. At late time points, spontaneous reactivation with apparent symptoms was characterized by a low Th2/Th1 marker ratio and a substantial induction of foxp3 reflecting the level of regulatory T cells. Characteristic gata3/tbx21 has potential as a biomarker for the status of mycobacterial disease.Milka Marjut HammarénKaisa Ester OksanenHanna Maria NisulaBruno Vincent LuukinenMarko PesuMika RämetMataleena ParikkaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 6, p e1004190 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Milka Marjut Hammarén Kaisa Ester Oksanen Hanna Maria Nisula Bruno Vincent Luukinen Marko Pesu Mika Rämet Mataleena Parikka Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
description	Tuberculosis is still a major health problem worldwide. Currently it is not known what kind of immune responses lead to successful control and clearance of Mycobacterium tuberculosis. This gap in knowledge is reflected by the inability to develop sufficient diagnostic and therapeutic tools to fight tuberculosis. We have used the Mycobacterium marinum infection model in the adult zebrafish and taken advantage of heterogeneity of zebrafish population to dissect the characteristics of adaptive immune responses, some of which are associated with well-controlled latency or bacterial clearance while others with progressive infection. Differences in T cell responses between subpopulations were measured at the transcriptional level. It was discovered that a high total T cell level was usually associated with lower bacterial loads alongside with a T helper 2 (Th2)-type gene expression signature. At late time points, spontaneous reactivation with apparent symptoms was characterized by a low Th2/Th1 marker ratio and a substantial induction of foxp3 reflecting the level of regulatory T cells. Characteristic gata3/tbx21 has potential as a biomarker for the status of mycobacterial disease.
format	article
author	Milka Marjut Hammarén Kaisa Ester Oksanen Hanna Maria Nisula Bruno Vincent Luukinen Marko Pesu Mika Rämet Mataleena Parikka
author_facet	Milka Marjut Hammarén Kaisa Ester Oksanen Hanna Maria Nisula Bruno Vincent Luukinen Marko Pesu Mika Rämet Mataleena Parikka
author_sort	Milka Marjut Hammarén
title	Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
title_short	Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
title_full	Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
title_fullStr	Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
title_full_unstemmed	Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
title_sort	adequate th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bc7c0be578d64990a76b8441036111c9
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Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish.

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