Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array

Abstract Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, we use microwestern array to rapidly evaluate suitable conditions for quantitative western blotting, with up to 192 antibody/dilution/replicate combinations on a sin...

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Autores principales: Rick J. Koch, Anne Marie Barrette, Alan D. Stern, Bin Hu, Mehdi Bouhaddou, Evren U. Azeloglu, Ravi Iyengar, Marc R. Birtwistle
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/bc7e4c10cd5a4850b9dd736a994f2554
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