CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells

Abstract Aspergillus (A.) fumigatus is an opportunistic fungal mold inducing invasive aspergillosis (IA) in immunocompromised patients. Although antifungal activity of human natural killer (NK) cells was shown in previous studies, the underlying cellular mechanisms and pathogen recognition receptors...

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Autores principales:	Sabrina Ziegler, Esther Weiss, Anna-Lena Schmitt, Jan Schlegel, Anne Burgert, Ulrich Terpitz, Markus Sauer, Lorenzo Moretta, Simona Sivori, Ines Leonhardt, Oliver Kurzai, Hermann Einsele, Juergen Loeffler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc7fb753dbbc427688eb5891267e29ec
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spelling	oai:doaj.org-article:bc7fb753dbbc427688eb5891267e29ec2021-12-02T12:32:02ZCD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells10.1038/s41598-017-06238-42045-2322https://doaj.org/article/bc7fb753dbbc427688eb5891267e29ec2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06238-4https://doaj.org/toc/2045-2322Abstract Aspergillus (A.) fumigatus is an opportunistic fungal mold inducing invasive aspergillosis (IA) in immunocompromised patients. Although antifungal activity of human natural killer (NK) cells was shown in previous studies, the underlying cellular mechanisms and pathogen recognition receptors (PRRs) are still unknown. Using flow cytometry we were able to show that the fluorescence positivity of the surface receptor CD56 significantly decreased upon fungal contact. To visualize the interaction site of NK cells and A. fumigatus we used SEM, CLSM and dSTORM techniques, which clearly demonstrated that NK cells directly interact with A. fumigatus via CD56 and that CD56 is re-organized and accumulated at this interaction site time-dependently. The inhibition of the cytoskeleton showed that the receptor re-organization was an active process dependent on actin re-arrangements. Furthermore, we could show that CD56 plays a role in the fungus mediated NK cell activation, since blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. These results confirmed the direct interaction of NK cells and A. fumigatus, leading to the conclusion that CD56 is a pathogen recognition receptor. These findings give new insights into the functional role of CD56 in the pathogen recognition during the innate immune response.Sabrina ZieglerEsther WeissAnna-Lena SchmittJan SchlegelAnne BurgertUlrich TerpitzMarkus SauerLorenzo MorettaSimona SivoriInes LeonhardtOliver KurzaiHermann EinseleJuergen LoefflerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabrina Ziegler Esther Weiss Anna-Lena Schmitt Jan Schlegel Anne Burgert Ulrich Terpitz Markus Sauer Lorenzo Moretta Simona Sivori Ines Leonhardt Oliver Kurzai Hermann Einsele Juergen Loeffler CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells
description	Abstract Aspergillus (A.) fumigatus is an opportunistic fungal mold inducing invasive aspergillosis (IA) in immunocompromised patients. Although antifungal activity of human natural killer (NK) cells was shown in previous studies, the underlying cellular mechanisms and pathogen recognition receptors (PRRs) are still unknown. Using flow cytometry we were able to show that the fluorescence positivity of the surface receptor CD56 significantly decreased upon fungal contact. To visualize the interaction site of NK cells and A. fumigatus we used SEM, CLSM and dSTORM techniques, which clearly demonstrated that NK cells directly interact with A. fumigatus via CD56 and that CD56 is re-organized and accumulated at this interaction site time-dependently. The inhibition of the cytoskeleton showed that the receptor re-organization was an active process dependent on actin re-arrangements. Furthermore, we could show that CD56 plays a role in the fungus mediated NK cell activation, since blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. These results confirmed the direct interaction of NK cells and A. fumigatus, leading to the conclusion that CD56 is a pathogen recognition receptor. These findings give new insights into the functional role of CD56 in the pathogen recognition during the innate immune response.
format	article
author	Sabrina Ziegler Esther Weiss Anna-Lena Schmitt Jan Schlegel Anne Burgert Ulrich Terpitz Markus Sauer Lorenzo Moretta Simona Sivori Ines Leonhardt Oliver Kurzai Hermann Einsele Juergen Loeffler
author_facet	Sabrina Ziegler Esther Weiss Anna-Lena Schmitt Jan Schlegel Anne Burgert Ulrich Terpitz Markus Sauer Lorenzo Moretta Simona Sivori Ines Leonhardt Oliver Kurzai Hermann Einsele Juergen Loeffler
author_sort	Sabrina Ziegler
title	CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells
title_short	CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells
title_full	CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells
title_fullStr	CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells
title_full_unstemmed	CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells
title_sort	cd56 is a pathogen recognition receptor on human natural killer cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc7fb753dbbc427688eb5891267e29ec
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CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells

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