2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology

2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterol...

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Autores principales:	Juliana G. S. de Lima, Daniel C. F. Lanza
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	2A peptide double-stranded RNA virus positive-sense single-stranded RNA virus Totiviridae Picornaviridae Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bc84e4a643474d03a09e0e79af9b0838
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spelling	oai:doaj.org-article:bc84e4a643474d03a09e0e79af9b08382021-11-25T19:12:55Z2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology10.3390/v131121601999-4915https://doaj.org/article/bc84e4a643474d03a09e0e79af9b08382021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2160https://doaj.org/toc/1999-49152A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.Juliana G. S. de LimaDaniel C. F. LanzaMDPI AGarticle2A peptidedouble-stranded RNA viruspositive-sense single-stranded RNA virusTotiviridaePicornaviridaeMicrobiologyQR1-502ENViruses, Vol 13, Iss 2160, p 2160 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	2A peptide double-stranded RNA virus positive-sense single-stranded RNA virus Totiviridae Picornaviridae Microbiology QR1-502
spellingShingle	2A peptide double-stranded RNA virus positive-sense single-stranded RNA virus Totiviridae Picornaviridae Microbiology QR1-502 Juliana G. S. de Lima Daniel C. F. Lanza 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
description	2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.
format	article
author	Juliana G. S. de Lima Daniel C. F. Lanza
author_facet	Juliana G. S. de Lima Daniel C. F. Lanza
author_sort	Juliana G. S. de Lima
title	2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
title_short	2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
title_full	2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
title_fullStr	2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
title_full_unstemmed	2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
title_sort	2a and 2a-like sequences: distribution in different virus species and applications in biotechnology
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bc84e4a643474d03a09e0e79af9b0838
work_keys_str_mv	AT julianagsdelima 2aand2alikesequencesdistributionindifferentvirusspeciesandapplicationsinbiotechnology AT danielcflanza 2aand2alikesequencesdistributionindifferentvirusspeciesandapplicationsinbiotechnology
_version_	1718410179460988928

2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology

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