The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.

Translational control and messenger RNA (mRNA) decay represent important control points in the regulation of gene expression. In yeast, the major pathway for mRNA decay is initiated by deadenylation followed by decapping and 5'-3' exonucleolytic digestion of the mRNA. Proteins that activat...

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Autores principales:	Thomas Sweet, Carrie Kovalak, Jeff Coller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bc893de7687542c5a6e1098188b678ce
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spelling	oai:doaj.org-article:bc893de7687542c5a6e1098188b678ce2021-11-18T05:36:37ZThe DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.1544-91731545-788510.1371/journal.pbio.1001342https://doaj.org/article/bc893de7687542c5a6e1098188b678ce2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22719226/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Translational control and messenger RNA (mRNA) decay represent important control points in the regulation of gene expression. In yeast, the major pathway for mRNA decay is initiated by deadenylation followed by decapping and 5'-3' exonucleolytic digestion of the mRNA. Proteins that activate decapping, such as the DEAD-box RNA helicase Dhh1, have been postulated to function by limiting translation initiation, thereby promoting a ribosome-free mRNA that is targeted for decapping. In contrast to this model, we show here that Dhh1 represses translation in vivo at a step subsequent to initiation. First, we establish that Dhh1 represses translation independent of initiation factors eIF4E and eIF3b. Second, we show association of Dhh1 on an mRNA leads to the accumulation of ribosomes on the transcript. Third, we demonstrate that endogenous Dhh1 accompanies slowly translocating polyribosomes. Lastly, Dhh1 activates decapping in response to impaired ribosome elongation. Together, these findings suggest that changes in ribosome transit rate represent a key event in the decapping and turnover of mRNA.Thomas SweetCarrie KovalakJeff CollerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 10, Iss 6, p e1001342 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Thomas Sweet Carrie Kovalak Jeff Coller The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.
description	Translational control and messenger RNA (mRNA) decay represent important control points in the regulation of gene expression. In yeast, the major pathway for mRNA decay is initiated by deadenylation followed by decapping and 5'-3' exonucleolytic digestion of the mRNA. Proteins that activate decapping, such as the DEAD-box RNA helicase Dhh1, have been postulated to function by limiting translation initiation, thereby promoting a ribosome-free mRNA that is targeted for decapping. In contrast to this model, we show here that Dhh1 represses translation in vivo at a step subsequent to initiation. First, we establish that Dhh1 represses translation independent of initiation factors eIF4E and eIF3b. Second, we show association of Dhh1 on an mRNA leads to the accumulation of ribosomes on the transcript. Third, we demonstrate that endogenous Dhh1 accompanies slowly translocating polyribosomes. Lastly, Dhh1 activates decapping in response to impaired ribosome elongation. Together, these findings suggest that changes in ribosome transit rate represent a key event in the decapping and turnover of mRNA.
format	article
author	Thomas Sweet Carrie Kovalak Jeff Coller
author_facet	Thomas Sweet Carrie Kovalak Jeff Coller
author_sort	Thomas Sweet
title	The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.
title_short	The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.
title_full	The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.
title_fullStr	The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.
title_full_unstemmed	The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.
title_sort	dead-box protein dhh1 promotes decapping by slowing ribosome movement.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bc893de7687542c5a6e1098188b678ce
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The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.

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