In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants

Ying Yang,1 Hai-yong Ao,1 Sheng-bing Yang,1 Yu-gang Wang,1 Wen-tao Lin,2 Zhi-feng Yu,1 Ting-ting Tang1 1Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 2Depart...

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Autores principales:	Yang Y, Ao HY, Yang SB, Wang YG, Lin WT, Yu ZF, Tang TT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	titanium nanotubes gentamicin implant-associated infection animal model Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bc896d18eefb4fb4af0435df13de7a06
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spelling	oai:doaj.org-article:bc896d18eefb4fb4af0435df13de7a062021-12-02T04:54:05ZIn vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants1178-2013https://doaj.org/article/bc896d18eefb4fb4af0435df13de7a062016-05-01T00:00:00Zhttps://www.dovepress.com/in-vivo-evaluation-of-the-anti-infection-potential-of-gentamicin-loade-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ying Yang,1 Hai-yong Ao,1 Sheng-bing Yang,1 Yu-gang Wang,1 Wen-tao Lin,2 Zhi-feng Yu,1 Ting-ting Tang1 1Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 2Department of Orthopedics, Fuzhou Second Hospital, Affiliated Hospital of Xiamen University, Fuzhou, People’s Republic of China Abstract: Titanium-based implants have been widely used in orthopedic surgery; however, failures still occur. Our in vitro study has demonstrated that gentamicin-loaded, 80 nm-diameter nanotubes possessed both antibacterial and osteogenic activities. Thus, the aim of this study was to further investigate the in vivo anti-infection effect of the titanium implants with gentamicin-loaded nanotubes. Thirty-six male Sprague Dawley rats were used to establish an implant-associated infection model. A volume of 50 µL Staphylococcus aureus suspension (1×105 CFU/mL) was injected into the medullary cavity of the left femur, and then the titanium rods without modification (Ti), titanium nanotubes without drug loading (NT), and gentamicin-loaded titanium nanotubes (NT-G) were inserted with phosphate-buffered saline-inoculated Ti rods as a blank control. X-ray images were obtained 1 day, 21 days, and 42 days after surgery; micro-computed tomography, microbiological, and histopathological analyses were used to evaluate the infections at the time of sacrifice. Radiographic signs of bone infection, including osteolysis, periosteal reaction, osteosclerosis, and damaged articular surfaces, were demonstrated in the infected Ti group and were slightly alleviated in the NT group but not observed in the NT-G group. Meanwhile, the radiographic and gross bone pathological scores of the NT-G group were significantly lower than those of the infected Ti group (P<0.01). Explant cultures revealed significantly less bacterial growth in the NT-G group than in the Ti and NT groups (P<0.01), and the NT group showed decreased live bacterial growth compared with the Ti group (P<0.01). Confocal laser scanning microscopy, scanning electron microscopy, and histopathological observations further confirmed decreased bacterial burden in the NT-G group compared with the Ti and NT groups. We concluded that the NT-G coatings can significantly prevent the development of implant-associated infections in a rat model; therefore, they may provide an effective drug-loading strategy to combat implant-associated infections in clinic. Keywords: titanium nanotubes, gentamicin, implant-associated infection, animal modelYang YAo HYYang SBWang YGLin WTYu ZFTang TTDove Medical Pressarticletitanium nanotubesgentamicinimplant-associated infectionanimal modelMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2223-2234 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	titanium nanotubes gentamicin implant-associated infection animal model Medicine (General) R5-920
spellingShingle	titanium nanotubes gentamicin implant-associated infection animal model Medicine (General) R5-920 Yang Y Ao HY Yang SB Wang YG Lin WT Yu ZF Tang TT In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
description	Ying Yang,1 Hai-yong Ao,1 Sheng-bing Yang,1 Yu-gang Wang,1 Wen-tao Lin,2 Zhi-feng Yu,1 Ting-ting Tang1 1Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 2Department of Orthopedics, Fuzhou Second Hospital, Affiliated Hospital of Xiamen University, Fuzhou, People’s Republic of China Abstract: Titanium-based implants have been widely used in orthopedic surgery; however, failures still occur. Our in vitro study has demonstrated that gentamicin-loaded, 80 nm-diameter nanotubes possessed both antibacterial and osteogenic activities. Thus, the aim of this study was to further investigate the in vivo anti-infection effect of the titanium implants with gentamicin-loaded nanotubes. Thirty-six male Sprague Dawley rats were used to establish an implant-associated infection model. A volume of 50 µL Staphylococcus aureus suspension (1×105 CFU/mL) was injected into the medullary cavity of the left femur, and then the titanium rods without modification (Ti), titanium nanotubes without drug loading (NT), and gentamicin-loaded titanium nanotubes (NT-G) were inserted with phosphate-buffered saline-inoculated Ti rods as a blank control. X-ray images were obtained 1 day, 21 days, and 42 days after surgery; micro-computed tomography, microbiological, and histopathological analyses were used to evaluate the infections at the time of sacrifice. Radiographic signs of bone infection, including osteolysis, periosteal reaction, osteosclerosis, and damaged articular surfaces, were demonstrated in the infected Ti group and were slightly alleviated in the NT group but not observed in the NT-G group. Meanwhile, the radiographic and gross bone pathological scores of the NT-G group were significantly lower than those of the infected Ti group (P<0.01). Explant cultures revealed significantly less bacterial growth in the NT-G group than in the Ti and NT groups (P<0.01), and the NT group showed decreased live bacterial growth compared with the Ti group (P<0.01). Confocal laser scanning microscopy, scanning electron microscopy, and histopathological observations further confirmed decreased bacterial burden in the NT-G group compared with the Ti and NT groups. We concluded that the NT-G coatings can significantly prevent the development of implant-associated infections in a rat model; therefore, they may provide an effective drug-loading strategy to combat implant-associated infections in clinic. Keywords: titanium nanotubes, gentamicin, implant-associated infection, animal model
format	article
author	Yang Y Ao HY Yang SB Wang YG Lin WT Yu ZF Tang TT
author_facet	Yang Y Ao HY Yang SB Wang YG Lin WT Yu ZF Tang TT
author_sort	Yang Y
title	In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
title_short	In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
title_full	In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
title_fullStr	In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
title_full_unstemmed	In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
title_sort	in vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/bc896d18eefb4fb4af0435df13de7a06
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In vivo evaluation of the anti-infection potential of gentamicin-loaded nanotubes on titania implants

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