Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

Abstract Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluores...

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Autores principales:	Shuntaro Ikeda, Ryouka Kawahara-Miki, Hisataka Iwata, Miki Sugimoto, Shinichi Kume
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc8da6bb4b7742a0af905ecd4e44e8e0
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spelling	oai:doaj.org-article:bc8da6bb4b7742a0af905ecd4e44e8e02021-12-02T15:05:31ZRole of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions10.1038/s41598-017-04003-12045-2322https://doaj.org/article/bc8da6bb4b7742a0af905ecd4e44e8e02017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04003-1https://doaj.org/toc/2045-2322Abstract Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluorescence analysis of bovine oocytes and preimplantation embryos revealed the consistent expression of MAT2A (the catalytic subunit of the ubiquitously expressed-type of MAT isozyme) during this period. Addition of the MAT2A inhibitor FIDAS to the culture media of bovine preimplantation embryos reduced their blastocyst development, revealing the particular importance of MAT2A in successful blastocyst development. Exploration of MAT2A-associated genomic regions in bovine blastocysts using chromatin immunoprecipitation and sequencing (ChIP-seq) identified candidate MAT2A-associated genes implicated not only in short-term periconceptional embryo development, but also in long-term phenotypic programming during this period in terms of growth, metabolism, and immune functions. These results suggest the critical involvement of MAT2A in the periconceptional period in life-long programming of health and disease as well as successful preimplantation development.Shuntaro IkedaRyouka Kawahara-MikiHisataka IwataMiki SugimotoShinichi KumeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuntaro Ikeda Ryouka Kawahara-Miki Hisataka Iwata Miki Sugimoto Shinichi Kume Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
description	Abstract Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluorescence analysis of bovine oocytes and preimplantation embryos revealed the consistent expression of MAT2A (the catalytic subunit of the ubiquitously expressed-type of MAT isozyme) during this period. Addition of the MAT2A inhibitor FIDAS to the culture media of bovine preimplantation embryos reduced their blastocyst development, revealing the particular importance of MAT2A in successful blastocyst development. Exploration of MAT2A-associated genomic regions in bovine blastocysts using chromatin immunoprecipitation and sequencing (ChIP-seq) identified candidate MAT2A-associated genes implicated not only in short-term periconceptional embryo development, but also in long-term phenotypic programming during this period in terms of growth, metabolism, and immune functions. These results suggest the critical involvement of MAT2A in the periconceptional period in life-long programming of health and disease as well as successful preimplantation development.
format	article
author	Shuntaro Ikeda Ryouka Kawahara-Miki Hisataka Iwata Miki Sugimoto Shinichi Kume
author_facet	Shuntaro Ikeda Ryouka Kawahara-Miki Hisataka Iwata Miki Sugimoto Shinichi Kume
author_sort	Shuntaro Ikeda
title	Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
title_short	Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
title_full	Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
title_fullStr	Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
title_full_unstemmed	Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
title_sort	role of methionine adenosyltransferase 2a in bovine preimplantation development and its associated genomic regions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc8da6bb4b7742a0af905ecd4e44e8e0
work_keys_str_mv	AT shuntaroikeda roleofmethionineadenosyltransferase2ainbovinepreimplantationdevelopmentanditsassociatedgenomicregions AT ryoukakawaharamiki roleofmethionineadenosyltransferase2ainbovinepreimplantationdevelopmentanditsassociatedgenomicregions AT hisatakaiwata roleofmethionineadenosyltransferase2ainbovinepreimplantationdevelopmentanditsassociatedgenomicregions AT mikisugimoto roleofmethionineadenosyltransferase2ainbovinepreimplantationdevelopmentanditsassociatedgenomicregions AT shinichikume roleofmethionineadenosyltransferase2ainbovinepreimplantationdevelopmentanditsassociatedgenomicregions
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Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

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