The flexibility and dynamics of the tubules in the endoplasmic reticulum

Abstract The endoplasmic reticulum (ER) is a single organelle in eukaryotic cells that extends throughout the cell and is involved in a large number of cellular functions. Using a combination of fixed and live cells (human MRC5 lung cells) in diffraction limited and super-resolved fluorescence micro...

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Autores principales:	Pantelis Georgiades, Victoria J. Allan, Graham D. Wright, Philip G. Woodman, Parinya Udommai, Manloeng A. Chung, Thomas A. Waigh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc9000eb178b46f8b13849ab3ee4c145
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spelling	oai:doaj.org-article:bc9000eb178b46f8b13849ab3ee4c1452021-12-02T15:06:12ZThe flexibility and dynamics of the tubules in the endoplasmic reticulum10.1038/s41598-017-16570-42045-2322https://doaj.org/article/bc9000eb178b46f8b13849ab3ee4c1452017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-16570-4https://doaj.org/toc/2045-2322Abstract The endoplasmic reticulum (ER) is a single organelle in eukaryotic cells that extends throughout the cell and is involved in a large number of cellular functions. Using a combination of fixed and live cells (human MRC5 lung cells) in diffraction limited and super-resolved fluorescence microscopy (STORM) experiments, we determined that the average persistence length of the ER tubules was 3.03 ± 0.24 μm. Removing the branched network junctions from the analysis caused a slight increase in the average persistence length to 4.71 ± 0.14 μm, and provides the tubule’s persistence length with a moderate length scale dependence. The average radius of the tubules was 44.1 ± 3.2 nm. The bending rigidity of the ER tubule membranes was found to be 10.9 ± 1.2 kT (17.0 ± 1.3 kT without branch points). We investigated the dynamic behaviour of ER tubules in live cells, and found that the ER tubules behaved like semi-flexible fibres under tension. The majority of the ER tubules experienced equilibrium transverse fluctuations under tension, whereas a minority number of them had active super-diffusive motions driven by motor proteins. Cells thus actively modulate the dynamics of the ER in a well-defined manner, which is expected in turn to impact on its many functions.Pantelis GeorgiadesVictoria J. AllanGraham D. WrightPhilip G. WoodmanParinya UdommaiManloeng A. ChungThomas A. WaighNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pantelis Georgiades Victoria J. Allan Graham D. Wright Philip G. Woodman Parinya Udommai Manloeng A. Chung Thomas A. Waigh The flexibility and dynamics of the tubules in the endoplasmic reticulum
description	Abstract The endoplasmic reticulum (ER) is a single organelle in eukaryotic cells that extends throughout the cell and is involved in a large number of cellular functions. Using a combination of fixed and live cells (human MRC5 lung cells) in diffraction limited and super-resolved fluorescence microscopy (STORM) experiments, we determined that the average persistence length of the ER tubules was 3.03 ± 0.24 μm. Removing the branched network junctions from the analysis caused a slight increase in the average persistence length to 4.71 ± 0.14 μm, and provides the tubule’s persistence length with a moderate length scale dependence. The average radius of the tubules was 44.1 ± 3.2 nm. The bending rigidity of the ER tubule membranes was found to be 10.9 ± 1.2 kT (17.0 ± 1.3 kT without branch points). We investigated the dynamic behaviour of ER tubules in live cells, and found that the ER tubules behaved like semi-flexible fibres under tension. The majority of the ER tubules experienced equilibrium transverse fluctuations under tension, whereas a minority number of them had active super-diffusive motions driven by motor proteins. Cells thus actively modulate the dynamics of the ER in a well-defined manner, which is expected in turn to impact on its many functions.
format	article
author	Pantelis Georgiades Victoria J. Allan Graham D. Wright Philip G. Woodman Parinya Udommai Manloeng A. Chung Thomas A. Waigh
author_facet	Pantelis Georgiades Victoria J. Allan Graham D. Wright Philip G. Woodman Parinya Udommai Manloeng A. Chung Thomas A. Waigh
author_sort	Pantelis Georgiades
title	The flexibility and dynamics of the tubules in the endoplasmic reticulum
title_short	The flexibility and dynamics of the tubules in the endoplasmic reticulum
title_full	The flexibility and dynamics of the tubules in the endoplasmic reticulum
title_fullStr	The flexibility and dynamics of the tubules in the endoplasmic reticulum
title_full_unstemmed	The flexibility and dynamics of the tubules in the endoplasmic reticulum
title_sort	flexibility and dynamics of the tubules in the endoplasmic reticulum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc9000eb178b46f8b13849ab3ee4c145
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The flexibility and dynamics of the tubules in the endoplasmic reticulum

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