A new cell-selective three-dimensional microincubator based on silicon photonic crystals.

In this work, we show that vertical, high aspect-ratio (HAR) photonic crystals (PhCs), consisting of periodic arrays of 5 µm wide gaps with depth of 50 µm separated by 3 µm thick silicon walls, fabricated by electrochemical micromachining, can be used as three-dimensional microincubators, allowing c...

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Autores principales:	Francesca Carpignano, Gloria Silva, Salvatore Surdo, Valentina Leva, Alessandra Montecucco, Francesca Aredia, Anna Ivana Scovassi, Sabina Merlo, Giuseppe Barillaro, Giuliano Mazzini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc9007b64a054d76884bb37de2d0b599
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id	oai:doaj.org-article:bc9007b64a054d76884bb37de2d0b599
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spelling	oai:doaj.org-article:bc9007b64a054d76884bb37de2d0b5992021-11-18T08:09:56ZA new cell-selective three-dimensional microincubator based on silicon photonic crystals.1932-620310.1371/journal.pone.0048556https://doaj.org/article/bc9007b64a054d76884bb37de2d0b5992012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139792/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In this work, we show that vertical, high aspect-ratio (HAR) photonic crystals (PhCs), consisting of periodic arrays of 5 µm wide gaps with depth of 50 µm separated by 3 µm thick silicon walls, fabricated by electrochemical micromachining, can be used as three-dimensional microincubators, allowing cell lines to be selectively grown into the gaps. Silicon micromachined dice incorporating regions with different surface profiles, namely flat silicon and deeply etched PhC, were used as microincubators for culturing adherent cell lines with different morphology and adhesion properties. We extensively investigated and compared the proliferative behavior on HAR PhCs of eight human cell models, with different origins, such as the epithelial (SW613-B3; HeLa; SW480; HCT116; HT29) and the mesenchymal (MRC-5V1; CF; HT1080). We also verified the contribution of cell sedimentation into the silicon gaps. Fluorescence microscopy analysis highlights that only cell lines that exhibit, in the tested culture condition, the behavior typical of the mesenchymal phenotype are able to penetrate into the gaps of the PhC, extending their body deeply in the narrow gaps between adjacent silicon walls, and to grow adherent to the vertical surfaces of silicon. Results reported in this work, confirmed in various experiments, strongly support our statement that such three-dimensional microstructures have selection capabilities with regard to the cell lines that can actively populate the narrow gaps. Cells with a mesenchymal phenotype could be exploited in the next future as bioreceptors, in combination with HAR PhC optical transducers, e.g., for label-free optical detection of cellular activities involving changes in cell adhesion and/or morphology (e.g., apoptosis) in a three-dimensional microenvironment.Francesca CarpignanoGloria SilvaSalvatore SurdoValentina LevaAlessandra MontecuccoFrancesca ArediaAnna Ivana ScovassiSabina MerloGiuseppe BarillaroGiuliano MazziniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48556 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesca Carpignano Gloria Silva Salvatore Surdo Valentina Leva Alessandra Montecucco Francesca Aredia Anna Ivana Scovassi Sabina Merlo Giuseppe Barillaro Giuliano Mazzini A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
description	In this work, we show that vertical, high aspect-ratio (HAR) photonic crystals (PhCs), consisting of periodic arrays of 5 µm wide gaps with depth of 50 µm separated by 3 µm thick silicon walls, fabricated by electrochemical micromachining, can be used as three-dimensional microincubators, allowing cell lines to be selectively grown into the gaps. Silicon micromachined dice incorporating regions with different surface profiles, namely flat silicon and deeply etched PhC, were used as microincubators for culturing adherent cell lines with different morphology and adhesion properties. We extensively investigated and compared the proliferative behavior on HAR PhCs of eight human cell models, with different origins, such as the epithelial (SW613-B3; HeLa; SW480; HCT116; HT29) and the mesenchymal (MRC-5V1; CF; HT1080). We also verified the contribution of cell sedimentation into the silicon gaps. Fluorescence microscopy analysis highlights that only cell lines that exhibit, in the tested culture condition, the behavior typical of the mesenchymal phenotype are able to penetrate into the gaps of the PhC, extending their body deeply in the narrow gaps between adjacent silicon walls, and to grow adherent to the vertical surfaces of silicon. Results reported in this work, confirmed in various experiments, strongly support our statement that such three-dimensional microstructures have selection capabilities with regard to the cell lines that can actively populate the narrow gaps. Cells with a mesenchymal phenotype could be exploited in the next future as bioreceptors, in combination with HAR PhC optical transducers, e.g., for label-free optical detection of cellular activities involving changes in cell adhesion and/or morphology (e.g., apoptosis) in a three-dimensional microenvironment.
format	article
author	Francesca Carpignano Gloria Silva Salvatore Surdo Valentina Leva Alessandra Montecucco Francesca Aredia Anna Ivana Scovassi Sabina Merlo Giuseppe Barillaro Giuliano Mazzini
author_facet	Francesca Carpignano Gloria Silva Salvatore Surdo Valentina Leva Alessandra Montecucco Francesca Aredia Anna Ivana Scovassi Sabina Merlo Giuseppe Barillaro Giuliano Mazzini
author_sort	Francesca Carpignano
title	A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
title_short	A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
title_full	A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
title_fullStr	A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
title_full_unstemmed	A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
title_sort	new cell-selective three-dimensional microincubator based on silicon photonic crystals.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bc9007b64a054d76884bb37de2d0b599
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A new cell-selective three-dimensional microincubator based on silicon photonic crystals.

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