Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect

Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect

Fei Yu,1,* Ming Li,1,* Zhipeng Yuan,2,* Feng Rao,1 Xingxing Fang,1 Baoguo Jiang,1 Yongqiang Wen,2 Peixun Zhang1 1Department of Orthopedics and Trauma, Peking University People’s Hospital, Beijing, China; 2School of Chemistry and Biological Engineering, University of Science and Technology...

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Autores principales: Yu F, Li M, Yuan Z, Rao F, Fang X, Jiang B, Wen Y, Zhang P
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2018
Materias:
resveratrol
PLA-Gelatin
nano-scaffold
PI3K/AKT signaling
cartilage defect
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/bc90b572ce294b898ce0305fa165ab9e
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spelling oai:doaj.org-article:bc90b572ce294b898ce0305fa165ab9e2021-12-02T00:39:22ZMechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect1178-2013https://doaj.org/article/bc90b572ce294b898ce0305fa165ab9e2018-11-01T00:00:00Zhttps://www.dovepress.com/mechanism-research-on-a-bioactive-resveratrol-pla-gelatin-porous-nano--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fei Yu,1,* Ming Li,1,* Zhipeng Yuan,2,* Feng Rao,1 Xingxing Fang,1 Baoguo Jiang,1 Yongqiang Wen,2 Peixun Zhang1 1Department of Orthopedics and Trauma, Peking University People’s Hospital, Beijing, China; 2School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China *These authors contributed equally to this work Background: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries.Purpose: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair.Methods: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone. Postmodel specimens and micro computed tomography (CT) were used to observe any macroscopic morphological changes in the articular cartilage and subchondral bone, whereas multiple staining methods were used to observe all microcosmic morphological changes. Gross scores and Mankin scores were used to evaluate the repair condition. Immunohistochemical staining was employed to detect protein expression.Results: When the repair included the resveratrol–PLA–gelatin porous nano-scaffold, the repaired cartilage and subchondral bone were in better condition. The expression levels of SIRT1, type II collagen, and PI3K/AKT signaling pathway-related proteins (AKT, VEGF, PTEN, Caspase 9, and MMP13) changed significantly. The expression levels of SIRT1,AKT and type II collagen proteins increased significantly, while the expression levels of VEGF, PTEN, Caspase9 and MMP13 proteins decreased significantly compared with the repair included blank porous PLA–gelatin nano-scaffold and without scaffold.Conclusion: We designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold with better performance, which promoted the repair of cartilage injury as a whole, and explained its possible mechanism in accelerating cartilage repair via the PI3K/AKT signaling pathway. Keywords: resveratrol, PLA–gelatin, nano-scaffold, PI3K/AKT signaling, cartilage defectYu FLi MYuan ZRao FFang XJiang BWen YZhang PDove Medical PressarticleresveratrolPLA-Gelatinnano-scaffoldPI3K/AKT signalingcartilage defectMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 7845-7858 (2018)
institution DOAJ
collection DOAJ
language EN
topic resveratrol
PLA-Gelatin
nano-scaffold
PI3K/AKT signaling
cartilage defect
Medicine (General)
R5-920
spellingShingle resveratrol
PLA-Gelatin
nano-scaffold
PI3K/AKT signaling
cartilage defect
Medicine (General)
R5-920
Yu F
Li M
Yuan Z
Rao F
Fang X
Jiang B
Wen Y
Zhang P
Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
description Fei Yu,1,* Ming Li,1,* Zhipeng Yuan,2,* Feng Rao,1 Xingxing Fang,1 Baoguo Jiang,1 Yongqiang Wen,2 Peixun Zhang1 1Department of Orthopedics and Trauma, Peking University People’s Hospital, Beijing, China; 2School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China *These authors contributed equally to this work Background: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries.Purpose: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair.Methods: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone. Postmodel specimens and micro computed tomography (CT) were used to observe any macroscopic morphological changes in the articular cartilage and subchondral bone, whereas multiple staining methods were used to observe all microcosmic morphological changes. Gross scores and Mankin scores were used to evaluate the repair condition. Immunohistochemical staining was employed to detect protein expression.Results: When the repair included the resveratrol–PLA–gelatin porous nano-scaffold, the repaired cartilage and subchondral bone were in better condition. The expression levels of SIRT1, type II collagen, and PI3K/AKT signaling pathway-related proteins (AKT, VEGF, PTEN, Caspase 9, and MMP13) changed significantly. The expression levels of SIRT1,AKT and type II collagen proteins increased significantly, while the expression levels of VEGF, PTEN, Caspase9 and MMP13 proteins decreased significantly compared with the repair included blank porous PLA–gelatin nano-scaffold and without scaffold.Conclusion: We designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold with better performance, which promoted the repair of cartilage injury as a whole, and explained its possible mechanism in accelerating cartilage repair via the PI3K/AKT signaling pathway. Keywords: resveratrol, PLA–gelatin, nano-scaffold, PI3K/AKT signaling, cartilage defect
format article
author Yu F
Li M
Yuan Z
Rao F
Fang X
Jiang B
Wen Y
Zhang P
author_facet Yu F
Li M
Yuan Z
Rao F
Fang X
Jiang B
Wen Y
Zhang P
author_sort Yu F
title Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_short Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_full Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_fullStr Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_full_unstemmed Mechanism research on a bioactive resveratrol–PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_sort mechanism research on a bioactive resveratrol–pla–gelatin porous nano-scaffold in promoting the repair of cartilage defect
publisher Dove Medical Press
publishDate 2018
url https://doaj.org/article/bc90b572ce294b898ce0305fa165ab9e
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