Antimicrobial effect of blue light using Porphyromonas gingivalis pigment

Abstract The development of antibiotics cannot keep up with the speed of resistance acquired by microorganisms. Recently, the development of antimicrobial photodynamic therapy (aPDT) has been a necessary antimicrobial strategy against antibiotic resistance. Among the wide variety of bacteria found i...

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Autores principales:	Ayaka Yoshida, Haruka Sasaki, Toshizo Toyama, Mitsunori Araki, Jun Fujioka, Koichi Tsukiyama, Nobushiro Hamada, Fumihiko Yoshino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc92e1502f1d43a987346c5971ddf672
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spelling	oai:doaj.org-article:bc92e1502f1d43a987346c5971ddf6722021-12-02T15:05:55ZAntimicrobial effect of blue light using Porphyromonas gingivalis pigment10.1038/s41598-017-05706-12045-2322https://doaj.org/article/bc92e1502f1d43a987346c5971ddf6722017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05706-1https://doaj.org/toc/2045-2322Abstract The development of antibiotics cannot keep up with the speed of resistance acquired by microorganisms. Recently, the development of antimicrobial photodynamic therapy (aPDT) has been a necessary antimicrobial strategy against antibiotic resistance. Among the wide variety of bacteria found in the oral flora, Porphyromonas gingivalis (P. gingivalis) is one of the etiological agents of periodontal disease. aPDT has been studied for periodontal disease, but has risks of cytotoxicity to normal stained tissue. In this study, we performed aPDT using protoporphyrin IX (PpIX), an intracellular pigment of P. gingivalis, without an external photosensitizer. We confirmed singlet oxygen generation by PpIX in a blue-light irradiation intensity-dependent manner. We discovered that blue-light irradiation on P. gingivalis is potentially bactericidal. The sterilization mechanism seems to be oxidative DNA damage in bacterial cells. Although it is said that no resistant bacteria will emerge using aPDT, the conventional method relies on an added photosensitizer dye. PpIX in P. gingivalis is used in energy production, so aPDT applied to PpIX of P. gingivalis should limit the appearance of resistant bacteria. This approach not only has potential as an effective treatment for new periodontal diseases, but also offers potential antibacterial treatment for multiple drug resistant bacteria.Ayaka YoshidaHaruka SasakiToshizo ToyamaMitsunori ArakiJun FujiokaKoichi TsukiyamaNobushiro HamadaFumihiko YoshinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayaka Yoshida Haruka Sasaki Toshizo Toyama Mitsunori Araki Jun Fujioka Koichi Tsukiyama Nobushiro Hamada Fumihiko Yoshino Antimicrobial effect of blue light using Porphyromonas gingivalis pigment
description	Abstract The development of antibiotics cannot keep up with the speed of resistance acquired by microorganisms. Recently, the development of antimicrobial photodynamic therapy (aPDT) has been a necessary antimicrobial strategy against antibiotic resistance. Among the wide variety of bacteria found in the oral flora, Porphyromonas gingivalis (P. gingivalis) is one of the etiological agents of periodontal disease. aPDT has been studied for periodontal disease, but has risks of cytotoxicity to normal stained tissue. In this study, we performed aPDT using protoporphyrin IX (PpIX), an intracellular pigment of P. gingivalis, without an external photosensitizer. We confirmed singlet oxygen generation by PpIX in a blue-light irradiation intensity-dependent manner. We discovered that blue-light irradiation on P. gingivalis is potentially bactericidal. The sterilization mechanism seems to be oxidative DNA damage in bacterial cells. Although it is said that no resistant bacteria will emerge using aPDT, the conventional method relies on an added photosensitizer dye. PpIX in P. gingivalis is used in energy production, so aPDT applied to PpIX of P. gingivalis should limit the appearance of resistant bacteria. This approach not only has potential as an effective treatment for new periodontal diseases, but also offers potential antibacterial treatment for multiple drug resistant bacteria.
format	article
author	Ayaka Yoshida Haruka Sasaki Toshizo Toyama Mitsunori Araki Jun Fujioka Koichi Tsukiyama Nobushiro Hamada Fumihiko Yoshino
author_facet	Ayaka Yoshida Haruka Sasaki Toshizo Toyama Mitsunori Araki Jun Fujioka Koichi Tsukiyama Nobushiro Hamada Fumihiko Yoshino
author_sort	Ayaka Yoshida
title	Antimicrobial effect of blue light using Porphyromonas gingivalis pigment
title_short	Antimicrobial effect of blue light using Porphyromonas gingivalis pigment
title_full	Antimicrobial effect of blue light using Porphyromonas gingivalis pigment
title_fullStr	Antimicrobial effect of blue light using Porphyromonas gingivalis pigment
title_full_unstemmed	Antimicrobial effect of blue light using Porphyromonas gingivalis pigment
title_sort	antimicrobial effect of blue light using porphyromonas gingivalis pigment
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc92e1502f1d43a987346c5971ddf672
work_keys_str_mv	AT ayakayoshida antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT harukasasaki antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT toshizotoyama antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT mitsunoriaraki antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT junfujioka antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT koichitsukiyama antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT nobushirohamada antimicrobialeffectofbluelightusingporphyromonasgingivalispigment AT fumihikoyoshino antimicrobialeffectofbluelightusingporphyromonasgingivalispigment
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Antimicrobial effect of blue light using Porphyromonas gingivalis pigment

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