Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers

Mei Z, Khan MA, Zhang X, Fu J. 2017. Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers. Biodiversitas 18: 1243-1249. For the genetic identification and authentication of living organisms, development of Sequenc...

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Autores principales:	ZHIQIANG MEI, MD. ASADUZZAMAN KHAN, XIANQIN ZHANG, JUNJIANG FU
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	genetic identification molecular markers penthorum chinense rapd sequence characterized amplified region Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bc985415d4c549dcb3906521fc55698b
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spelling	oai:doaj.org-article:bc985415d4c549dcb3906521fc55698b2021-11-16T13:56:34ZShort Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers1412-033X2085-472210.13057/biodiv/d180349https://doaj.org/article/bc985415d4c549dcb3906521fc55698b2017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2049https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Mei Z, Khan MA, Zhang X, Fu J. 2017. Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers. Biodiversitas 18: 1243-1249. For the genetic identification and authentication of living organisms, development of Sequence-Characterized Amplified Region (SCAR) markers from Random Amplified Polymorphic DNA (RAPD) fragments is a valuable molecular approach. By using SCAR markers, molecular analysis is simplified to a Polymerase Chain Reaction (PCR) analysis using PCR primers designed from specific sequences of the RAPD amplicons. In this study, RAPD fragments from improved RAPD amplification of a perennial herb Penthorum chinense Pursh from China were cloned into a T-vector, and positive clones were identified by PCR amplification, and sequenced with the Sanger sequencing method for the SCAR marker development. Five SCAR markers were developed that were very specific to P. chinense, and deposited in GenBank (accession numbers: KX671029, KX671030, KX671031, KX671032 and KX671033). BLAST searches of these five nucleotide sequences in the GenBank database showed no identity with markers from other species. This study was developing five specific SCAR markers, has enabled reliable genetic identification of the herbal plant species Penthorum chinense Pursh, useful for authenticating future samples of this important medicinal herb.ZHIQIANG MEIMD. ASADUZZAMAN KHANXIANQIN ZHANGJUNJIANG FUMBI & UNS Soloarticlegenetic identificationmolecular markerspenthorum chinenserapdsequence characterized amplified regionBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1243-1249 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic identification molecular markers penthorum chinense rapd sequence characterized amplified region Biology (General) QH301-705.5
spellingShingle	genetic identification molecular markers penthorum chinense rapd sequence characterized amplified region Biology (General) QH301-705.5 ZHIQIANG MEI MD. ASADUZZAMAN KHAN XIANQIN ZHANG JUNJIANG FU Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers
description	Mei Z, Khan MA, Zhang X, Fu J. 2017. Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers. Biodiversitas 18: 1243-1249. For the genetic identification and authentication of living organisms, development of Sequence-Characterized Amplified Region (SCAR) markers from Random Amplified Polymorphic DNA (RAPD) fragments is a valuable molecular approach. By using SCAR markers, molecular analysis is simplified to a Polymerase Chain Reaction (PCR) analysis using PCR primers designed from specific sequences of the RAPD amplicons. In this study, RAPD fragments from improved RAPD amplification of a perennial herb Penthorum chinense Pursh from China were cloned into a T-vector, and positive clones were identified by PCR amplification, and sequenced with the Sanger sequencing method for the SCAR marker development. Five SCAR markers were developed that were very specific to P. chinense, and deposited in GenBank (accession numbers: KX671029, KX671030, KX671031, KX671032 and KX671033). BLAST searches of these five nucleotide sequences in the GenBank database showed no identity with markers from other species. This study was developing five specific SCAR markers, has enabled reliable genetic identification of the herbal plant species Penthorum chinense Pursh, useful for authenticating future samples of this important medicinal herb.
format	article
author	ZHIQIANG MEI MD. ASADUZZAMAN KHAN XIANQIN ZHANG JUNJIANG FU
author_facet	ZHIQIANG MEI MD. ASADUZZAMAN KHAN XIANQIN ZHANG JUNJIANG FU
author_sort	ZHIQIANG MEI
title	Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers
title_short	Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers
title_full	Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers
title_fullStr	Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers
title_full_unstemmed	Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers
title_sort	short communication: rapid and accurate genetic authentication of penthorum chinense by improved rapd-derived species-specific scar markers
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/bc985415d4c549dcb3906521fc55698b
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Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers

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