Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism

Zhiwen Zhang1,2, Xiaoling Fang1, Junguo Hao1, Yajuan Li1, Xianyi Sha1 1Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, People&rsquo;s Republic of China; 2Shanghai Institute of Materia Medica, C...

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Autores principales:	Zhang ZW, Fang XL, Hao JG, Li YJ, Sha XY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bc9b8186536a4b848a9a4c75c33aec4e
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spelling	oai:doaj.org-article:bc9b8186536a4b848a9a4c75c33aec4e2021-12-02T02:53:45ZTriolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism1176-91141178-2013https://doaj.org/article/bc9b8186536a4b848a9a4c75c33aec4e2011-10-01T00:00:00Zhttp://www.dovepress.com/triolein-based-polycation-lipid-nanocarrier-for-efficient-gene-deliver-a8423https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhiwen Zhang1,2, Xiaoling Fang1, Junguo Hao1, Yajuan Li1, Xianyi Sha1 1Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, People&rsquo;s Republic of China; 2Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People&rsquo;s Republic of China Abstract: We proposed to develop a polycation lipid nanocarrier (PLN) with higher transfection efficiency than our previously described polycation nanostrucutred lipid nanocarrier (PNLC). PLN was composed of triolein, cetylated low-molecular-weight polyethylenimine, and dioleoyl phosphatidylethanolamine. The physicochemical properties of PLN and the PLN/DNA complexes (PDC) were characterized. The in vitro transfection was performed in human lung adenocarcinoma (SPC-A1) cells, and the intracellular mechanism was investigated as well. The measurements indicated that PLN and PDC are homogenous nanometer-sized particles with a positive charge. The transfection efficiency of PDC significantly increased with the content of triolein and was higher than that of PNLC and commercial LipofectamineTM 2000. In particular, the transfection of PLN in the presence of 10% serum was more effective than that in its absence. With the help of specific inhibitors of chlorpromazine and filipin, the clathrin-dependent endocytosis pathway was determined to be the main contributor to the successful transfection mediated by PLN in SPC-A1 cells. The captured images verified that the fluorescent PDC was localized in the lysosomes and nuclei after endocytosis. Thus, PLN represents a novel efficient nonviral gene delivery vector. Keywords: triolein, dioleoyl phosphatidylethanolalmine, polyethylenimine, lipid nanocarrier, mechanismZhang ZWFang XLHao JGLi YJSha XYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2235-2244 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang ZW Fang XL Hao JG Li YJ Sha XY Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
description	Zhiwen Zhang1,2, Xiaoling Fang1, Junguo Hao1, Yajuan Li1, Xianyi Sha1 1Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, People&rsquo;s Republic of China; 2Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People&rsquo;s Republic of China Abstract: We proposed to develop a polycation lipid nanocarrier (PLN) with higher transfection efficiency than our previously described polycation nanostrucutred lipid nanocarrier (PNLC). PLN was composed of triolein, cetylated low-molecular-weight polyethylenimine, and dioleoyl phosphatidylethanolamine. The physicochemical properties of PLN and the PLN/DNA complexes (PDC) were characterized. The in vitro transfection was performed in human lung adenocarcinoma (SPC-A1) cells, and the intracellular mechanism was investigated as well. The measurements indicated that PLN and PDC are homogenous nanometer-sized particles with a positive charge. The transfection efficiency of PDC significantly increased with the content of triolein and was higher than that of PNLC and commercial LipofectamineTM 2000. In particular, the transfection of PLN in the presence of 10% serum was more effective than that in its absence. With the help of specific inhibitors of chlorpromazine and filipin, the clathrin-dependent endocytosis pathway was determined to be the main contributor to the successful transfection mediated by PLN in SPC-A1 cells. The captured images verified that the fluorescent PDC was localized in the lysosomes and nuclei after endocytosis. Thus, PLN represents a novel efficient nonviral gene delivery vector. Keywords: triolein, dioleoyl phosphatidylethanolalmine, polyethylenimine, lipid nanocarrier, mechanism
format	article
author	Zhang ZW Fang XL Hao JG Li YJ Sha XY
author_facet	Zhang ZW Fang XL Hao JG Li YJ Sha XY
author_sort	Zhang ZW
title	Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
title_short	Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
title_full	Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
title_fullStr	Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
title_full_unstemmed	Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
title_sort	triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/bc9b8186536a4b848a9a4c75c33aec4e
work_keys_str_mv	AT zhangzw trioleinbasedpolycationlipidnanocarrierforefficientgenedeliverycharacteristicsandmechanism AT fangxl trioleinbasedpolycationlipidnanocarrierforefficientgenedeliverycharacteristicsandmechanism AT haojg trioleinbasedpolycationlipidnanocarrierforefficientgenedeliverycharacteristicsandmechanism AT liyj trioleinbasedpolycationlipidnanocarrierforefficientgenedeliverycharacteristicsandmechanism AT shaxy trioleinbasedpolycationlipidnanocarrierforefficientgenedeliverycharacteristicsandmechanism
_version_	1718402066306564096

Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism

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