Highly efficient and specific genome editing in silkworm using custom TALENs.

Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction and application of transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era for genome editing. We des...

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Autores principales:	Sanyuan Ma, Shengling Zhang, Feng Wang, Yong Liu, Yuanyuan Liu, Hanfu Xu, Chun Liu, Ying Lin, Ping Zhao, Qingyou Xia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc9eafc5d6bf4076bf84d583230b6438
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spelling	oai:doaj.org-article:bc9eafc5d6bf4076bf84d583230b64382021-11-18T07:05:13ZHighly efficient and specific genome editing in silkworm using custom TALENs.1932-620310.1371/journal.pone.0045035https://doaj.org/article/bc9eafc5d6bf4076bf84d583230b64382012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028749/?tool=EBIhttps://doaj.org/toc/1932-6203Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction and application of transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era for genome editing. We describe the production of two active TALENs and their successful application in the targeted mutagenesis of silkworm, Bombyx mori, whose genetic manipulation methods are parallel to those of Drosophila and other insects. We will also show that the simultaneous expression of two pairs of TALENs generates heritable large chromosomal deletion. Our results demonstrate that (i) TALENs can be used in silkworm and (ii) heritable large chromosomal deletions can be induced by two pairs of TALENs in whole organisms. The generation and the high frequency of TALENs-induced targeted mutagenesis in silkworm will promote the genetic modification of silkworm and other insect species.Sanyuan MaShengling ZhangFeng WangYong LiuYuanyuan LiuHanfu XuChun LiuYing LinPing ZhaoQingyou XiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45035 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanyuan Ma Shengling Zhang Feng Wang Yong Liu Yuanyuan Liu Hanfu Xu Chun Liu Ying Lin Ping Zhao Qingyou Xia Highly efficient and specific genome editing in silkworm using custom TALENs.
description	Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction and application of transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era for genome editing. We describe the production of two active TALENs and their successful application in the targeted mutagenesis of silkworm, Bombyx mori, whose genetic manipulation methods are parallel to those of Drosophila and other insects. We will also show that the simultaneous expression of two pairs of TALENs generates heritable large chromosomal deletion. Our results demonstrate that (i) TALENs can be used in silkworm and (ii) heritable large chromosomal deletions can be induced by two pairs of TALENs in whole organisms. The generation and the high frequency of TALENs-induced targeted mutagenesis in silkworm will promote the genetic modification of silkworm and other insect species.
format	article
author	Sanyuan Ma Shengling Zhang Feng Wang Yong Liu Yuanyuan Liu Hanfu Xu Chun Liu Ying Lin Ping Zhao Qingyou Xia
author_facet	Sanyuan Ma Shengling Zhang Feng Wang Yong Liu Yuanyuan Liu Hanfu Xu Chun Liu Ying Lin Ping Zhao Qingyou Xia
author_sort	Sanyuan Ma
title	Highly efficient and specific genome editing in silkworm using custom TALENs.
title_short	Highly efficient and specific genome editing in silkworm using custom TALENs.
title_full	Highly efficient and specific genome editing in silkworm using custom TALENs.
title_fullStr	Highly efficient and specific genome editing in silkworm using custom TALENs.
title_full_unstemmed	Highly efficient and specific genome editing in silkworm using custom TALENs.
title_sort	highly efficient and specific genome editing in silkworm using custom talens.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bc9eafc5d6bf4076bf84d583230b6438
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_version_	1718424004157505536

Highly efficient and specific genome editing in silkworm using custom TALENs.

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