Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content

Abstract Coumarin and its derivatives are widely used as fragrances in industrial products and have medical value. The goal of the present study was to discover genes and pathways related to coumarin biosynthesis in Melilotus albus using transcriptome analysis. The genes of five M. albus near-isogen...

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Autores principales:	Kai Luo, Fan Wu, Daiyu Zhang, Rui Dong, Zhichao Fan, Rui Zhang, Zhuanzhuan Yan, Yanrong Wang, Jiyu Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bca3b05babc14d71b90a590408f9e76c
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spelling	oai:doaj.org-article:bca3b05babc14d71b90a590408f9e76c2021-12-02T15:06:07ZTranscriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content10.1038/s41598-017-04111-y2045-2322https://doaj.org/article/bca3b05babc14d71b90a590408f9e76c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04111-yhttps://doaj.org/toc/2045-2322Abstract Coumarin and its derivatives are widely used as fragrances in industrial products and have medical value. The goal of the present study was to discover genes and pathways related to coumarin biosynthesis in Melilotus albus using transcriptome analysis. The genes of five M. albus near-isogenic lines (NILs) that had different coumarin content and β-glucosidase activity according to the investigation of pedigree were quantified and then analysed by RNA-Seq. Using transcriptome analysis, differentially expressed genes (DEGs) were identified in two pairwise comparisons that differed in coumarin content as well as in two pairwise comparisons that differed in β-glucosidase activity. Gene expression pattern analysis suggested similar transcriptional trends in the genotypes with the same coumarin levels. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database of DEGs was used to identify functional pathways associated with coumarin biosynthesis. We identified 111 unigenes, with several DEGs among them possibly being related to coumarin synthesis pathways. Unigenes encoding a hexokinase, an abscisic acid receptor, a phenylalanine ammonia-lyase (PAL) and two peroxidases particularly showed correspondence with the coumarin content of different genotypes. These results will contribute to a better understanding of the coumarin biosynthesis in M. albus.Kai LuoFan WuDaiyu ZhangRui DongZhichao FanRui ZhangZhuanzhuan YanYanrong WangJiyu ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Luo Fan Wu Daiyu Zhang Rui Dong Zhichao Fan Rui Zhang Zhuanzhuan Yan Yanrong Wang Jiyu Zhang Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content
description	Abstract Coumarin and its derivatives are widely used as fragrances in industrial products and have medical value. The goal of the present study was to discover genes and pathways related to coumarin biosynthesis in Melilotus albus using transcriptome analysis. The genes of five M. albus near-isogenic lines (NILs) that had different coumarin content and β-glucosidase activity according to the investigation of pedigree were quantified and then analysed by RNA-Seq. Using transcriptome analysis, differentially expressed genes (DEGs) were identified in two pairwise comparisons that differed in coumarin content as well as in two pairwise comparisons that differed in β-glucosidase activity. Gene expression pattern analysis suggested similar transcriptional trends in the genotypes with the same coumarin levels. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database of DEGs was used to identify functional pathways associated with coumarin biosynthesis. We identified 111 unigenes, with several DEGs among them possibly being related to coumarin synthesis pathways. Unigenes encoding a hexokinase, an abscisic acid receptor, a phenylalanine ammonia-lyase (PAL) and two peroxidases particularly showed correspondence with the coumarin content of different genotypes. These results will contribute to a better understanding of the coumarin biosynthesis in M. albus.
format	article
author	Kai Luo Fan Wu Daiyu Zhang Rui Dong Zhichao Fan Rui Zhang Zhuanzhuan Yan Yanrong Wang Jiyu Zhang
author_facet	Kai Luo Fan Wu Daiyu Zhang Rui Dong Zhichao Fan Rui Zhang Zhuanzhuan Yan Yanrong Wang Jiyu Zhang
author_sort	Kai Luo
title	Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content
title_short	Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content
title_full	Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content
title_fullStr	Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content
title_full_unstemmed	Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content
title_sort	transcriptomic profiling of melilotus albus near-isogenic lines contrasting for coumarin content
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bca3b05babc14d71b90a590408f9e76c
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Transcriptomic profiling of Melilotus albus near-isogenic lines contrasting for coumarin content

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