SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>

Several methodologies have been proposed in order to establish anticancer action, mainly focused on in vitro cytotoxicity valuation on neoplasic cell lines derived from human cancer. However, most of these cell lines are metabolically incompetent, restricting model sensibility and generating false n...

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Auteurs principaux:	Stephanie O. PRIETO, Fabio A. ARISTIZÁBAL G.
Format:	article
Langue:	EN
Publié:	Universidad de Antioquia 2009
Sujets:	cytochrome Hep-G2 enzyme inducer cytotoxicity MTT resazurina. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Accès en ligne:	https://doaj.org/article/bca96ac0ab6d40b48a742f43e51f71bb
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spelling	oai:doaj.org-article:bca96ac0ab6d40b48a742f43e51f71bb2021-11-19T04:15:44ZSENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>0121-40042145-2660https://doaj.org/article/bca96ac0ab6d40b48a742f43e51f71bb2009-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/1933https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Several methodologies have been proposed in order to establish anticancer action, mainly focused on in vitro cytotoxicity valuation on neoplasic cell lines derived from human cancer. However, most of these cell lines are metabolically incompetent, restricting model sensibility and generating false negative answers. Hep-G2 cell line is widely used because of its high sensibility reflecting phase I and II activity of some enzymes of phase I and II, which play an important role in activation and detoxification of xenobiotics. In this study, Hep-G2 is used as a model to find cytotoxic activity produced by 2-amino- 1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) and Cyclophosphamide, in presence or absence of inductor agents. This analysis was carried out by measuring indirectly viable number of cells with assays like MTT and resazurine staining. In addition, the possibility of co-cultures with other cell lines was evaluated according to strengthen method sensibility. Cytotoxicity of PhIP and cyclophosphamide was observed with the cellular staining methods. For this reason a co-culture system was established. The answer was similar to independent cell lines. These results propose that pre-treated Hep-G2 cells with some inductive agents show sensibility by PhIP as different authors postulate. Stephanie O. PRIETOFabio A. ARISTIZÁBAL G.Universidad de AntioquiaarticlecytochromeHep-G2enzyme inducercytotoxicityMTTresazurina.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 16, Iss 2 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytochrome Hep-G2 enzyme inducer cytotoxicity MTT resazurina. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	cytochrome Hep-G2 enzyme inducer cytotoxicity MTT resazurina. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Stephanie O. PRIETO Fabio A. ARISTIZÁBAL G. SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>
description	Several methodologies have been proposed in order to establish anticancer action, mainly focused on in vitro cytotoxicity valuation on neoplasic cell lines derived from human cancer. However, most of these cell lines are metabolically incompetent, restricting model sensibility and generating false negative answers. Hep-G2 cell line is widely used because of its high sensibility reflecting phase I and II activity of some enzymes of phase I and II, which play an important role in activation and detoxification of xenobiotics. In this study, Hep-G2 is used as a model to find cytotoxic activity produced by 2-amino- 1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) and Cyclophosphamide, in presence or absence of inductor agents. This analysis was carried out by measuring indirectly viable number of cells with assays like MTT and resazurine staining. In addition, the possibility of co-cultures with other cell lines was evaluated according to strengthen method sensibility. Cytotoxicity of PhIP and cyclophosphamide was observed with the cellular staining methods. For this reason a co-culture system was established. The answer was similar to independent cell lines. These results propose that pre-treated Hep-G2 cells with some inductive agents show sensibility by PhIP as different authors postulate.
format	article
author	Stephanie O. PRIETO Fabio A. ARISTIZÁBAL G.
author_facet	Stephanie O. PRIETO Fabio A. ARISTIZÁBAL G.
author_sort	Stephanie O. PRIETO
title	SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>
title_short	SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>
title_full	SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>
title_fullStr	SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>
title_full_unstemmed	SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>
title_sort	sensitivility profile hep-g2 as a model of cytotoxic activity determination of bioactivated xenobiotics via cyp <sub>450</sub>
publisher	Universidad de Antioquia
publishDate	2009
url	https://doaj.org/article/bca96ac0ab6d40b48a742f43e51f71bb
work_keys_str_mv	AT stephanieoprieto sensitivilityprofilehepg2asamodelofcytotoxicactivitydeterminationofbioactivatedxenobioticsviacypsub450sub AT fabioaaristizabalg sensitivilityprofilehepg2asamodelofcytotoxicactivitydeterminationofbioactivatedxenobioticsviacypsub450sub
_version_	1718420466460262400

SENSITIVILITY PROFILE HEP-G2 AS A MODEL OF CYTOTOXIC ACTIVITY DETERMINATION OF BIOACTIVATED XENOBIOTICS VIA CYP <sub>450</sub>

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