A survey of the sorghum transcriptome using single-molecule long reads

Alternative splicing and alternative polyadenylation (APA) contribute to mRNA diversity but are difficult to assess using short read RNA-seq data. Here, the authors use single molecule long-read isoform sequencing and develop a computational pipeline to identify full-length splice isoforms and APA s...

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Autores principales:	Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur, Anireddy S. N. Reddy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bcaa70a959d04e6ca2c3634b67b09163
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spelling	oai:doaj.org-article:bcaa70a959d04e6ca2c3634b67b091632021-12-02T15:35:43ZA survey of the sorghum transcriptome using single-molecule long reads10.1038/ncomms117062041-1723https://doaj.org/article/bcaa70a959d04e6ca2c3634b67b091632016-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms11706https://doaj.org/toc/2041-1723Alternative splicing and alternative polyadenylation (APA) contribute to mRNA diversity but are difficult to assess using short read RNA-seq data. Here, the authors use single molecule long-read isoform sequencing and develop a computational pipeline to identify full-length splice isoforms and APA sites in sorghum.Salah E. Abdel-GhanyMichael HamiltonJennifer L. JacobiPeter NgamNicholas DevittFaye SchilkeyAsa Ben-HurAnireddy S. N. ReddyNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Salah E. Abdel-Ghany Michael Hamilton Jennifer L. Jacobi Peter Ngam Nicholas Devitt Faye Schilkey Asa Ben-Hur Anireddy S. N. Reddy A survey of the sorghum transcriptome using single-molecule long reads
description	Alternative splicing and alternative polyadenylation (APA) contribute to mRNA diversity but are difficult to assess using short read RNA-seq data. Here, the authors use single molecule long-read isoform sequencing and develop a computational pipeline to identify full-length splice isoforms and APA sites in sorghum.
format	article
author	Salah E. Abdel-Ghany Michael Hamilton Jennifer L. Jacobi Peter Ngam Nicholas Devitt Faye Schilkey Asa Ben-Hur Anireddy S. N. Reddy
author_facet	Salah E. Abdel-Ghany Michael Hamilton Jennifer L. Jacobi Peter Ngam Nicholas Devitt Faye Schilkey Asa Ben-Hur Anireddy S. N. Reddy
author_sort	Salah E. Abdel-Ghany
title	A survey of the sorghum transcriptome using single-molecule long reads
title_short	A survey of the sorghum transcriptome using single-molecule long reads
title_full	A survey of the sorghum transcriptome using single-molecule long reads
title_fullStr	A survey of the sorghum transcriptome using single-molecule long reads
title_full_unstemmed	A survey of the sorghum transcriptome using single-molecule long reads
title_sort	survey of the sorghum transcriptome using single-molecule long reads
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/bcaa70a959d04e6ca2c3634b67b09163
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A survey of the sorghum transcriptome using single-molecule long reads

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