Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.

Infection of non-enveloped polyomaviruses depends on an intact microtubular network. Here we focus on mouse polyomavirus (MPyV). We show that the dynamics of MPyV cytoplasmic transport reflects the characteristics of microtubular motor-driven transport with bi-directional saltatory movements. In cel...

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Autores principales:	Vojtech Zila, Francesco Difato, Lucie Klimova, Sandra Huerfano, Jitka Forstova
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bcbbd0c8638a469b840181288c86c9e3
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spelling	oai:doaj.org-article:bcbbd0c8638a469b840181288c86c9e32021-11-18T08:20:08ZInvolvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.1932-620310.1371/journal.pone.0096922https://doaj.org/article/bcbbd0c8638a469b840181288c86c9e32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24810588/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Infection of non-enveloped polyomaviruses depends on an intact microtubular network. Here we focus on mouse polyomavirus (MPyV). We show that the dynamics of MPyV cytoplasmic transport reflects the characteristics of microtubular motor-driven transport with bi-directional saltatory movements. In cells treated with microtubule-disrupting agents, localization of MPyV was significantly perturbed, the virus was retained at the cell periphery, mostly within membrane structures resembling multicaveolar complexes, and at later times post-infection, only a fraction of the virus was found in Rab7-positive endosomes and multivesicular bodies. Inhibition of cytoplasmic dynein-based motility by overexpression of dynamitin affected perinuclear translocation of the virus, delivery of virions to the ER and substantially reduced the numbers of infected cells, while overexpression of dominant-negative form of kinesin-1 or kinesin-2 had no significant impact on virus localization and infectivity. We also found that transport along microtubules was important for MPyV-containing endosome sequential acquisition of Rab5, Rab7 and Rab11 GTPases. However, in contrast to dominant-negative mutant of Rab7 (T22N), overexpression of dominant-negative mutant Rab11 (S25N) did not affect the virus infectivity. Altogether, our study revealed that MPyV cytoplasmic trafficking leading to productive infection bypasses recycling endosomes, does not require the function of kinesin-1 and kinesin-2, but depends on functional dynein-mediated transport along microtubules for translocation of the virions from peripheral, often caveolin-positive compartments to late endosomes and ER - a prerequisite for efficient delivery of the viral genome to the nucleus.Vojtech ZilaFrancesco DifatoLucie KlimovaSandra HuerfanoJitka ForstovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96922 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vojtech Zila Francesco Difato Lucie Klimova Sandra Huerfano Jitka Forstova Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
description	Infection of non-enveloped polyomaviruses depends on an intact microtubular network. Here we focus on mouse polyomavirus (MPyV). We show that the dynamics of MPyV cytoplasmic transport reflects the characteristics of microtubular motor-driven transport with bi-directional saltatory movements. In cells treated with microtubule-disrupting agents, localization of MPyV was significantly perturbed, the virus was retained at the cell periphery, mostly within membrane structures resembling multicaveolar complexes, and at later times post-infection, only a fraction of the virus was found in Rab7-positive endosomes and multivesicular bodies. Inhibition of cytoplasmic dynein-based motility by overexpression of dynamitin affected perinuclear translocation of the virus, delivery of virions to the ER and substantially reduced the numbers of infected cells, while overexpression of dominant-negative form of kinesin-1 or kinesin-2 had no significant impact on virus localization and infectivity. We also found that transport along microtubules was important for MPyV-containing endosome sequential acquisition of Rab5, Rab7 and Rab11 GTPases. However, in contrast to dominant-negative mutant of Rab7 (T22N), overexpression of dominant-negative mutant Rab11 (S25N) did not affect the virus infectivity. Altogether, our study revealed that MPyV cytoplasmic trafficking leading to productive infection bypasses recycling endosomes, does not require the function of kinesin-1 and kinesin-2, but depends on functional dynein-mediated transport along microtubules for translocation of the virions from peripheral, often caveolin-positive compartments to late endosomes and ER - a prerequisite for efficient delivery of the viral genome to the nucleus.
format	article
author	Vojtech Zila Francesco Difato Lucie Klimova Sandra Huerfano Jitka Forstova
author_facet	Vojtech Zila Francesco Difato Lucie Klimova Sandra Huerfano Jitka Forstova
author_sort	Vojtech Zila
title	Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
title_short	Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
title_full	Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
title_fullStr	Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
title_full_unstemmed	Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
title_sort	involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bcbbd0c8638a469b840181288c86c9e3
work_keys_str_mv	AT vojtechzila involvementofmicrotubularnetworkanditsmotorsinproductiveendocytictraffickingofmousepolyomavirus AT francescodifato involvementofmicrotubularnetworkanditsmotorsinproductiveendocytictraffickingofmousepolyomavirus AT lucieklimova involvementofmicrotubularnetworkanditsmotorsinproductiveendocytictraffickingofmousepolyomavirus AT sandrahuerfano involvementofmicrotubularnetworkanditsmotorsinproductiveendocytictraffickingofmousepolyomavirus AT jitkaforstova involvementofmicrotubularnetworkanditsmotorsinproductiveendocytictraffickingofmousepolyomavirus
_version_	1718421947722760192

Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.

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