c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120

c-di-GMP is a ubiquitous bacterial signal regulating various physiological process. Anabaena PCC 7120 (Anabaena) is a filamentous cyanobacterium able to form regularly-spaced heterocysts for nitrogen fixation, in response to combined-nitrogen deprivation in 24h. Anabaena possesses 16 genes encoding...

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Autores principales:	Min Huang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cyanobacteria c-di-GMP nitrogen fixation signal transduction homeostasis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bcc4ca2d234d41258172cf688f2c24e2
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spelling	oai:doaj.org-article:bcc4ca2d234d41258172cf688f2c24e22021-12-03T17:47:00Zc-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 71201664-302X10.3389/fmicb.2021.793336https://doaj.org/article/bcc4ca2d234d41258172cf688f2c24e22021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.793336/fullhttps://doaj.org/toc/1664-302Xc-di-GMP is a ubiquitous bacterial signal regulating various physiological process. Anabaena PCC 7120 (Anabaena) is a filamentous cyanobacterium able to form regularly-spaced heterocysts for nitrogen fixation, in response to combined-nitrogen deprivation in 24h. Anabaena possesses 16 genes encoding proteins for c-di-GMP metabolism, and their functions are poorly characterized, except all2874 (cdgS) whose deletion causes a decrease in heterocyst frequency 48h after nitrogen starvation. We demonstrated here that c-di-GMP levels increased significantly in Anabaena after combined-nitrogen starvation. By inactivating each of the 16 genes, we found that the deletion of all1175 (cdgSH) led to an increase of heterocyst frequency 24h after nitrogen stepdown. A double mutant ΔcdgSHΔcdgS had an additive effect over the single mutants in regulating heterocyst frequency, indicating that the two genes acted at different time points for heterocyst spacing. Biochemical and genetic data further showed that the functions of CdgSH and CdgS in the setup or maintenance of heterocyst frequency depended on their opposing effects on the intracellular levels of c-di-GMP. Finally, we demonstrated that heterocyst differentiation was completely inhibited when c-di-GMP levels became too high or too low. Together, these results indicate that the homeostasis of c-di-GMP level is important for heterocyst differentiation in Anabaena.Min HuangMin HuangJu-Yuan ZhangXiaoli ZengCheng-Cai ZhangCheng-Cai ZhangCheng-Cai ZhangCheng-Cai ZhangFrontiers Media S.A.articlecyanobacteriac-di-GMPnitrogen fixationsignal transductionhomeostasisMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyanobacteria c-di-GMP nitrogen fixation signal transduction homeostasis Microbiology QR1-502
spellingShingle	cyanobacteria c-di-GMP nitrogen fixation signal transduction homeostasis Microbiology QR1-502 Min Huang Min Huang Ju-Yuan Zhang Xiaoli Zeng Cheng-Cai Zhang Cheng-Cai Zhang Cheng-Cai Zhang Cheng-Cai Zhang c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120
description	c-di-GMP is a ubiquitous bacterial signal regulating various physiological process. Anabaena PCC 7120 (Anabaena) is a filamentous cyanobacterium able to form regularly-spaced heterocysts for nitrogen fixation, in response to combined-nitrogen deprivation in 24h. Anabaena possesses 16 genes encoding proteins for c-di-GMP metabolism, and their functions are poorly characterized, except all2874 (cdgS) whose deletion causes a decrease in heterocyst frequency 48h after nitrogen starvation. We demonstrated here that c-di-GMP levels increased significantly in Anabaena after combined-nitrogen starvation. By inactivating each of the 16 genes, we found that the deletion of all1175 (cdgSH) led to an increase of heterocyst frequency 24h after nitrogen stepdown. A double mutant ΔcdgSHΔcdgS had an additive effect over the single mutants in regulating heterocyst frequency, indicating that the two genes acted at different time points for heterocyst spacing. Biochemical and genetic data further showed that the functions of CdgSH and CdgS in the setup or maintenance of heterocyst frequency depended on their opposing effects on the intracellular levels of c-di-GMP. Finally, we demonstrated that heterocyst differentiation was completely inhibited when c-di-GMP levels became too high or too low. Together, these results indicate that the homeostasis of c-di-GMP level is important for heterocyst differentiation in Anabaena.
format	article
author	Min Huang Min Huang Ju-Yuan Zhang Xiaoli Zeng Cheng-Cai Zhang Cheng-Cai Zhang Cheng-Cai Zhang Cheng-Cai Zhang
author_facet	Min Huang Min Huang Ju-Yuan Zhang Xiaoli Zeng Cheng-Cai Zhang Cheng-Cai Zhang Cheng-Cai Zhang Cheng-Cai Zhang
author_sort	Min Huang
title	c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120
title_short	c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120
title_full	c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120
title_fullStr	c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120
title_full_unstemmed	c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120
title_sort	c-di-gmp homeostasis is critical for heterocyst development in anabaena sp. pcc 7120
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/bcc4ca2d234d41258172cf688f2c24e2
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c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120

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