Improving genetic transformation rates in honeybees

Abstract Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a sep...

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Autores principales: M. Otte, O. Netschitailo, O. Kaftanoglu, Y. Wang, R. E. Page Jr., M. Beye
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Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/bcc971f2498a424c9c1b95dbcf111497
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spelling oai:doaj.org-article:bcc971f2498a424c9c1b95dbcf1114972021-12-02T15:08:11ZImproving genetic transformation rates in honeybees10.1038/s41598-018-34724-w2045-2322https://doaj.org/article/bcc971f2498a424c9c1b95dbcf1114972018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34724-whttps://doaj.org/toc/2045-2322Abstract Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a separate honeybee colony. Here, we report on further improvement of the transformation rate in honeybees by using a combination of different procedures. We employed a hyperactive transposase protein (hyPBaseapis), we tripled the amount of injected transposase mRNAs and we injected embryos into the first third (anterior part) of the embryo. These three improvements together doubled the transformation rate from 19% to 44%. We propose that the hyperactive transposase (hyPBaseapis) and the other steps used may also help to improve the transformation rates in other species in which screening and crossing procedures are laborious.M. OtteO. NetschitailoO. KaftanogluY. WangR. E. Page Jr.M. BeyeNature PortfolioarticleIncreased Transformation RateHyperactive TransposaseTransposase mRNAAnterior InjectionTransposon CassetteMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
institution DOAJ
collection DOAJ
language EN
topic Increased Transformation Rate
Hyperactive Transposase
Transposase mRNA
Anterior Injection
Transposon Cassette
Medicine
R
Science
Q
spellingShingle Increased Transformation Rate
Hyperactive Transposase
Transposase mRNA
Anterior Injection
Transposon Cassette
Medicine
R
Science
Q
M. Otte
O. Netschitailo
O. Kaftanoglu
Y. Wang
R. E. Page Jr.
M. Beye
Improving genetic transformation rates in honeybees
description Abstract Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a separate honeybee colony. Here, we report on further improvement of the transformation rate in honeybees by using a combination of different procedures. We employed a hyperactive transposase protein (hyPBaseapis), we tripled the amount of injected transposase mRNAs and we injected embryos into the first third (anterior part) of the embryo. These three improvements together doubled the transformation rate from 19% to 44%. We propose that the hyperactive transposase (hyPBaseapis) and the other steps used may also help to improve the transformation rates in other species in which screening and crossing procedures are laborious.
format article
author M. Otte
O. Netschitailo
O. Kaftanoglu
Y. Wang
R. E. Page Jr.
M. Beye
author_facet M. Otte
O. Netschitailo
O. Kaftanoglu
Y. Wang
R. E. Page Jr.
M. Beye
author_sort M. Otte
title Improving genetic transformation rates in honeybees
title_short Improving genetic transformation rates in honeybees
title_full Improving genetic transformation rates in honeybees
title_fullStr Improving genetic transformation rates in honeybees
title_full_unstemmed Improving genetic transformation rates in honeybees
title_sort improving genetic transformation rates in honeybees
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/bcc971f2498a424c9c1b95dbcf111497
work_keys_str_mv AT motte improvinggenetictransformationratesinhoneybees
AT onetschitailo improvinggenetictransformationratesinhoneybees
AT okaftanoglu improvinggenetictransformationratesinhoneybees
AT ywang improvinggenetictransformationratesinhoneybees
AT repagejr improvinggenetictransformationratesinhoneybees
AT mbeye improvinggenetictransformationratesinhoneybees
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