Improving genetic transformation rates in honeybees

Abstract Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a sep...

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Auteurs principaux:	M. Otte, O. Netschitailo, O. Kaftanoglu, Y. Wang, R. E. Page Jr., M. Beye
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Increased Transformation Rate Hyperactive Transposase Transposase mRNA Anterior Injection Transposon Cassette Medicine R Science Q
Accès en ligne:	https://doaj.org/article/bcc971f2498a424c9c1b95dbcf111497
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spelling	oai:doaj.org-article:bcc971f2498a424c9c1b95dbcf1114972021-12-02T15:08:11ZImproving genetic transformation rates in honeybees10.1038/s41598-018-34724-w2045-2322https://doaj.org/article/bcc971f2498a424c9c1b95dbcf1114972018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34724-whttps://doaj.org/toc/2045-2322Abstract Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a separate honeybee colony. Here, we report on further improvement of the transformation rate in honeybees by using a combination of different procedures. We employed a hyperactive transposase protein (hyPBaseapis), we tripled the amount of injected transposase mRNAs and we injected embryos into the first third (anterior part) of the embryo. These three improvements together doubled the transformation rate from 19% to 44%. We propose that the hyperactive transposase (hyPBaseapis) and the other steps used may also help to improve the transformation rates in other species in which screening and crossing procedures are laborious.M. OtteO. NetschitailoO. KaftanogluY. WangR. E. Page Jr.M. BeyeNature PortfolioarticleIncreased Transformation RateHyperactive TransposaseTransposase mRNAAnterior InjectionTransposon CassetteMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Increased Transformation Rate Hyperactive Transposase Transposase mRNA Anterior Injection Transposon Cassette Medicine R Science Q
spellingShingle	Increased Transformation Rate Hyperactive Transposase Transposase mRNA Anterior Injection Transposon Cassette Medicine R Science Q M. Otte O. Netschitailo O. Kaftanoglu Y. Wang R. E. Page Jr. M. Beye Improving genetic transformation rates in honeybees
description	Abstract Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a separate honeybee colony. Here, we report on further improvement of the transformation rate in honeybees by using a combination of different procedures. We employed a hyperactive transposase protein (hyPBaseapis), we tripled the amount of injected transposase mRNAs and we injected embryos into the first third (anterior part) of the embryo. These three improvements together doubled the transformation rate from 19% to 44%. We propose that the hyperactive transposase (hyPBaseapis) and the other steps used may also help to improve the transformation rates in other species in which screening and crossing procedures are laborious.
format	article
author	M. Otte O. Netschitailo O. Kaftanoglu Y. Wang R. E. Page Jr. M. Beye
author_facet	M. Otte O. Netschitailo O. Kaftanoglu Y. Wang R. E. Page Jr. M. Beye
author_sort	M. Otte
title	Improving genetic transformation rates in honeybees
title_short	Improving genetic transformation rates in honeybees
title_full	Improving genetic transformation rates in honeybees
title_fullStr	Improving genetic transformation rates in honeybees
title_full_unstemmed	Improving genetic transformation rates in honeybees
title_sort	improving genetic transformation rates in honeybees
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/bcc971f2498a424c9c1b95dbcf111497
work_keys_str_mv	AT motte improvinggenetictransformationratesinhoneybees AT onetschitailo improvinggenetictransformationratesinhoneybees AT okaftanoglu improvinggenetictransformationratesinhoneybees AT ywang improvinggenetictransformationratesinhoneybees AT repagejr improvinggenetictransformationratesinhoneybees AT mbeye improvinggenetictransformationratesinhoneybees
_version_	1718388276996341760

Improving genetic transformation rates in honeybees

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