Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis

Abstract Vaccination against bovine mastitis lags behind despite high demand from the dairy industry and margin for efficacy improvement. We previously compared two immunization protocols against E. coli using either only the intramuscular route or a combination of intramuscular and mammary ductal r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nathan Cebron, Sarah Maman, Sarah Walachowski, Blandine Gausserès, Patricia Cunha, Pascal Rainard, Gilles Foucras
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/bcd237681531408eaca7734d7331fa53
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bcd237681531408eaca7734d7331fa53
record_format	dspace
spelling	oai:doaj.org-article:bcd237681531408eaca7734d7331fa532021-12-02T11:50:55ZTh17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis10.1038/s41541-020-00258-42059-0105https://doaj.org/article/bcd237681531408eaca7734d7331fa532020-11-01T00:00:00Zhttps://doi.org/10.1038/s41541-020-00258-4https://doaj.org/toc/2059-0105Abstract Vaccination against bovine mastitis lags behind despite high demand from the dairy industry and margin for efficacy improvement. We previously compared two immunization protocols against E. coli using either only the intramuscular route or a combination of intramuscular and mammary ductal routes, also known as ‘prime and pull’ strategy. A homologous mammary challenge during the memory phase showed that immunization favorably modified the mastitis course, notably in locally immunized cows in comparison to intramuscular and control adjuvant-only groups. Here, we performed whole-blood profiling through RNA-seq transcriptome and plasma cytokine 15-plex analyses at time points of the E. coli mastitis that showed significant clinical and laboratory differences among the groups. Diminished production of inflammatory cytokines and increased IFNγ were detected in the blood of immunized cows, where a T lymphocyte activation profile was evidenced at 12-h post infection. Acute phase neutropenia was less severe in these cows, and pathways related to neutrophil diapedesis and monocyte activation were also present. Furthermore, three intramammary-immunized cows showing faster healing and shorter mastitis duration had gene profiles that differed from their counterparts, but without any clue for the mastitis susceptibility difference. Inasmuch, when gene expression of CD4 T cells was assessed in mammary tissue, enrichment of IL-17-associated pathways was identified in the quarters of intramammary-immunized cows not only after challenge but also in the control quarters that were not infected. These findings indicate that local immunization mobilizes protective mechanisms that rely on the settlement of type 3 immunity-related CD4 T cells prior to infection.Nathan CebronSarah MamanSarah WalachowskiBlandine GausserèsPatricia CunhaPascal RainardGilles FoucrasNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 5, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Nathan Cebron Sarah Maman Sarah Walachowski Blandine Gausserès Patricia Cunha Pascal Rainard Gilles Foucras Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis
description	Abstract Vaccination against bovine mastitis lags behind despite high demand from the dairy industry and margin for efficacy improvement. We previously compared two immunization protocols against E. coli using either only the intramuscular route or a combination of intramuscular and mammary ductal routes, also known as ‘prime and pull’ strategy. A homologous mammary challenge during the memory phase showed that immunization favorably modified the mastitis course, notably in locally immunized cows in comparison to intramuscular and control adjuvant-only groups. Here, we performed whole-blood profiling through RNA-seq transcriptome and plasma cytokine 15-plex analyses at time points of the E. coli mastitis that showed significant clinical and laboratory differences among the groups. Diminished production of inflammatory cytokines and increased IFNγ were detected in the blood of immunized cows, where a T lymphocyte activation profile was evidenced at 12-h post infection. Acute phase neutropenia was less severe in these cows, and pathways related to neutrophil diapedesis and monocyte activation were also present. Furthermore, three intramammary-immunized cows showing faster healing and shorter mastitis duration had gene profiles that differed from their counterparts, but without any clue for the mastitis susceptibility difference. Inasmuch, when gene expression of CD4 T cells was assessed in mammary tissue, enrichment of IL-17-associated pathways was identified in the quarters of intramammary-immunized cows not only after challenge but also in the control quarters that were not infected. These findings indicate that local immunization mobilizes protective mechanisms that rely on the settlement of type 3 immunity-related CD4 T cells prior to infection.
format	article
author	Nathan Cebron Sarah Maman Sarah Walachowski Blandine Gausserès Patricia Cunha Pascal Rainard Gilles Foucras
author_facet	Nathan Cebron Sarah Maman Sarah Walachowski Blandine Gausserès Patricia Cunha Pascal Rainard Gilles Foucras
author_sort	Nathan Cebron
title	Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis
title_short	Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis
title_full	Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis
title_fullStr	Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis
title_full_unstemmed	Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis
title_sort	th17-related mammary immunity, but not a high systemic th1 immune response is associated with protection against e. coli mastitis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/bcd237681531408eaca7734d7331fa53
work_keys_str_mv	AT nathancebron th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis AT sarahmaman th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis AT sarahwalachowski th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis AT blandinegausseres th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis AT patriciacunha th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis AT pascalrainard th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis AT gillesfoucras th17relatedmammaryimmunitybutnotahighsystemicth1immuneresponseisassociatedwithprotectionagainstecolimastitis
_version_	1718395188061143040

Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis

Ejemplares similares