Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic

Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and...

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Autores principales:	Fei Ye, Yan Wang, Qijian He, Zhaoshuo Wang, Enyue Ma, Shiliang Zhu, Heling Yu, Huadong Yin, Xiaoling Zhao, Diyan Li, Hengyong Xu, Hua Li, Qing Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	alv-j data-independent acquisition proteomic chicken Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/bcd9eb3d9b8f4fb1b4ff9191358b67d5
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spelling	oai:doaj.org-article:bcd9eb3d9b8f4fb1b4ff9191358b67d52021-11-17T14:21:58ZScreening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic2150-55942150-560810.1080/21505594.2020.1809323https://doaj.org/article/bcd9eb3d9b8f4fb1b4ff9191358b67d52020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1809323https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry.Fei YeYan WangQijian HeZhaoshuo WangEnyue MaShiliang ZhuHeling YuHuadong YinXiaoling ZhaoDiyan LiHengyong XuHua LiQing ZhuTaylor & Francis Grouparticlealv-jdata-independent acquisitionproteomicchickenInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1158-1176 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	alv-j data-independent acquisition proteomic chicken Infectious and parasitic diseases RC109-216
spellingShingle	alv-j data-independent acquisition proteomic chicken Infectious and parasitic diseases RC109-216 Fei Ye Yan Wang Qijian He Zhaoshuo Wang Enyue Ma Shiliang Zhu Heling Yu Huadong Yin Xiaoling Zhao Diyan Li Hengyong Xu Hua Li Qing Zhu Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
description	Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry.
format	article
author	Fei Ye Yan Wang Qijian He Zhaoshuo Wang Enyue Ma Shiliang Zhu Heling Yu Huadong Yin Xiaoling Zhao Diyan Li Hengyong Xu Hua Li Qing Zhu
author_facet	Fei Ye Yan Wang Qijian He Zhaoshuo Wang Enyue Ma Shiliang Zhu Heling Yu Huadong Yin Xiaoling Zhao Diyan Li Hengyong Xu Hua Li Qing Zhu
author_sort	Fei Ye
title	Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_short	Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_full	Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_fullStr	Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_full_unstemmed	Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_sort	screening of immune biomarkers in different breeds of chickens infected with j subgroup of avian leukemia virus by proteomic
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/bcd9eb3d9b8f4fb1b4ff9191358b67d5
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Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic

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