Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
Abstract Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA...
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Nature Portfolio
2021
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oai:doaj.org-article:bce1a1b8bf434bd4a1df0871f8556f482021-12-02T17:24:01ZExtracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs10.1038/s41598-021-97753-y2045-2322https://doaj.org/article/bce1a1b8bf434bd4a1df0871f8556f482021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97753-yhttps://doaj.org/toc/2045-2322Abstract Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling of EVs released from two distinct cell lines and treated dendritic cells derived from circulating monocytes (mono-DCs) with these EVs. We confirmed the internalization of EVs by mono-DCs and the down-regulation of microRNA mRNA targets in treated mono-DCs. Differences in surface markers of dendritic cells cultivated in the presence of EVs indicated that their content disrupts the maturation process. Additionally, microRNAs known to interfere with dendritic cell function, and detected in EVs, matched microRNAs from squamous cell carcinoma patients’ plasma: miR-17-5p in oropharyngeal squamous cell carcinoma, miR-21 in oral squamous cell carcinoma, miR-16, miR-24, and miR-181a circulating in both oral and oropharyngeal squamous cell carcinoma, and miR-23b, which has not been previously described in plasma of head and neck squamous cell carcinoma, was found in plasma from patients with these cancer subtypes. This study contributes with insights on EVs in signaling between cancer and immune cells in squamous cell carcinoma of the head and neck.Elisangela de Paula SilvaLuciana Cavalheiro MartiFlávia Maziero AndreghettoRomário Oliveira de SalesMartin HobermanBárbara dos Santos DiasLarissa Figueiredo Alves DinizAlessandro Marins dos SantosRaquel Ajub MoysesOtávio Alberto CurioniRossana Veronica Mendoza LopezFabio Daumas NunesEloiza Helena TajaraPatricia SeverinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
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Medicine R Science Q Elisangela de Paula Silva Luciana Cavalheiro Marti Flávia Maziero Andreghetto Romário Oliveira de Sales Martin Hoberman Bárbara dos Santos Dias Larissa Figueiredo Alves Diniz Alessandro Marins dos Santos Raquel Ajub Moyses Otávio Alberto Curioni Rossana Veronica Mendoza Lopez Fabio Daumas Nunes Eloiza Helena Tajara Patricia Severino Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
| description |
Abstract Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling of EVs released from two distinct cell lines and treated dendritic cells derived from circulating monocytes (mono-DCs) with these EVs. We confirmed the internalization of EVs by mono-DCs and the down-regulation of microRNA mRNA targets in treated mono-DCs. Differences in surface markers of dendritic cells cultivated in the presence of EVs indicated that their content disrupts the maturation process. Additionally, microRNAs known to interfere with dendritic cell function, and detected in EVs, matched microRNAs from squamous cell carcinoma patients’ plasma: miR-17-5p in oropharyngeal squamous cell carcinoma, miR-21 in oral squamous cell carcinoma, miR-16, miR-24, and miR-181a circulating in both oral and oropharyngeal squamous cell carcinoma, and miR-23b, which has not been previously described in plasma of head and neck squamous cell carcinoma, was found in plasma from patients with these cancer subtypes. This study contributes with insights on EVs in signaling between cancer and immune cells in squamous cell carcinoma of the head and neck. |
| format |
article |
| author |
Elisangela de Paula Silva Luciana Cavalheiro Marti Flávia Maziero Andreghetto Romário Oliveira de Sales Martin Hoberman Bárbara dos Santos Dias Larissa Figueiredo Alves Diniz Alessandro Marins dos Santos Raquel Ajub Moyses Otávio Alberto Curioni Rossana Veronica Mendoza Lopez Fabio Daumas Nunes Eloiza Helena Tajara Patricia Severino |
| author_facet |
Elisangela de Paula Silva Luciana Cavalheiro Marti Flávia Maziero Andreghetto Romário Oliveira de Sales Martin Hoberman Bárbara dos Santos Dias Larissa Figueiredo Alves Diniz Alessandro Marins dos Santos Raquel Ajub Moyses Otávio Alberto Curioni Rossana Veronica Mendoza Lopez Fabio Daumas Nunes Eloiza Helena Tajara Patricia Severino |
| author_sort |
Elisangela de Paula Silva |
| title |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
| title_short |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
| title_full |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
| title_fullStr |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
| title_full_unstemmed |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
| title_sort |
extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma micrornas |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/bce1a1b8bf434bd4a1df0871f8556f48 |
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