Toxic effects of selected proprietary dry eye drops on Acanthamoeba

Abstract Amoebae of the genus Acanthamoeba are ubiquitous protists that have been isolated from many sources such as soils, water and the air. They are responsible for infections including fatal encephalitis and a severe keratitis in humans. To date, there is no satisfactorily effective therapeutic...

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Autores principales:	Ines Sifaoui, María Reyes-Batlle, Atteneri López-Arencibia, Olfa Chiboub, Javier Rodríguez-Martín, Pedro Rocha-Cabrera, Basilio Valladares, José E. Piñero, Jacob Lorenzo-Morales
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bce44a01607e42229dc9037c6e401588
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spelling	oai:doaj.org-article:bce44a01607e42229dc9037c6e4015882021-12-02T15:08:44ZToxic effects of selected proprietary dry eye drops on Acanthamoeba10.1038/s41598-018-26914-32045-2322https://doaj.org/article/bce44a01607e42229dc9037c6e4015882018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26914-3https://doaj.org/toc/2045-2322Abstract Amoebae of the genus Acanthamoeba are ubiquitous protists that have been isolated from many sources such as soils, water and the air. They are responsible for infections including fatal encephalitis and a severe keratitis in humans. To date, there is no satisfactorily effective therapeutic agent against this pathogen and the infections it causes are exacerbated by the existence of a resistant cyst stage produced by this amoeba. As dry eye syndrome is a risk factor for Acanthamoeba keratitis, we aimed to evaluate the anti-Acanthamoeba activity of a variety of proprietary eye drops intended to treat dry eye syndrome. From the nine eye drop formulations tested, “Systane Ultra” was determined to be the most active against all tested Acanthamoeba strains. During our investigations into the mode of action of Systane Ultra, we discovered that it decreases mitochondrial membrane potential and ATP levels, induces chromatin condensation, and increases the permeability of the plasma-membrane.Ines SifaouiMaría Reyes-BatlleAtteneri López-ArencibiaOlfa ChiboubJavier Rodríguez-MartínPedro Rocha-CabreraBasilio ValladaresJosé E. PiñeroJacob Lorenzo-MoralesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ines Sifaoui María Reyes-Batlle Atteneri López-Arencibia Olfa Chiboub Javier Rodríguez-Martín Pedro Rocha-Cabrera Basilio Valladares José E. Piñero Jacob Lorenzo-Morales Toxic effects of selected proprietary dry eye drops on Acanthamoeba
description	Abstract Amoebae of the genus Acanthamoeba are ubiquitous protists that have been isolated from many sources such as soils, water and the air. They are responsible for infections including fatal encephalitis and a severe keratitis in humans. To date, there is no satisfactorily effective therapeutic agent against this pathogen and the infections it causes are exacerbated by the existence of a resistant cyst stage produced by this amoeba. As dry eye syndrome is a risk factor for Acanthamoeba keratitis, we aimed to evaluate the anti-Acanthamoeba activity of a variety of proprietary eye drops intended to treat dry eye syndrome. From the nine eye drop formulations tested, “Systane Ultra” was determined to be the most active against all tested Acanthamoeba strains. During our investigations into the mode of action of Systane Ultra, we discovered that it decreases mitochondrial membrane potential and ATP levels, induces chromatin condensation, and increases the permeability of the plasma-membrane.
format	article
author	Ines Sifaoui María Reyes-Batlle Atteneri López-Arencibia Olfa Chiboub Javier Rodríguez-Martín Pedro Rocha-Cabrera Basilio Valladares José E. Piñero Jacob Lorenzo-Morales
author_facet	Ines Sifaoui María Reyes-Batlle Atteneri López-Arencibia Olfa Chiboub Javier Rodríguez-Martín Pedro Rocha-Cabrera Basilio Valladares José E. Piñero Jacob Lorenzo-Morales
author_sort	Ines Sifaoui
title	Toxic effects of selected proprietary dry eye drops on Acanthamoeba
title_short	Toxic effects of selected proprietary dry eye drops on Acanthamoeba
title_full	Toxic effects of selected proprietary dry eye drops on Acanthamoeba
title_fullStr	Toxic effects of selected proprietary dry eye drops on Acanthamoeba
title_full_unstemmed	Toxic effects of selected proprietary dry eye drops on Acanthamoeba
title_sort	toxic effects of selected proprietary dry eye drops on acanthamoeba
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/bce44a01607e42229dc9037c6e401588
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Toxic effects of selected proprietary dry eye drops on Acanthamoeba

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