Promising plasmid DNA vector based on APTES-modified silica nanoparticles

Tuck-yun Cheang1,*, Bing Tang1,*, An-wu Xu2, Guang-qi Chang1, Zuo-jun Hu1, Wei-ling He1, Zhou-hao Xing2, Jian-bo Xu1, Mian Wang1, Shen-ming Wang11Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; 2Division of Nanomaterials and Chemistry, Hefei...

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Autores principales:	Cheang TY, Tang B, Xu AW, Chang GQ, Hu ZJ, He WL, Xing ZH, Xu JB, Wang M, Wang SM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bce4bde9a396456492b272c09633261b
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spelling	oai:doaj.org-article:bce4bde9a396456492b272c09633261b2021-12-02T05:02:08ZPromising plasmid DNA vector based on APTES-modified silica nanoparticles1176-91141178-2013https://doaj.org/article/bce4bde9a396456492b272c09633261b2012-02-01T00:00:00Zhttp://www.dovepress.com/promising-plasmid-dna-vector-based-on-aptes-modified-silica-nanopartic-a9330https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Tuck-yun Cheang1,, Bing Tang1,, An-wu Xu2, Guang-qi Chang1, Zuo-jun Hu1, Wei-ling He1, Zhou-hao Xing2, Jian-bo Xu1, Mian Wang1, Shen-ming Wang11Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; 2Division of Nanomaterials and Chemistry, Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, China&nbsp; *Both authors contributed equally to this workAbstract: Nanoparticles have an enormous potential for development in biomedical applications, such as gene or drug delivery. We developed and characterized aminopropyltriethoxysilane-functionalized silicon dioxide nanoparticles (APTES-SiNPs) for gene therapy. Lipofectamine&reg; 2000, a commonly used agent, served as a contrast. We showed that APTES-SiNPs had a gene transfection efficiency almost equal to that of Lipofectamine 2000, but with lower cytotoxicity. Thus, these novel APTES-SiNPs can achieve highly efficient transfection of plasmid DNA, and to some extent reduce cytotoxicity, which might overcome the critical drawbacks in vivo of conventional carriers, such as viral vectors, organic polymers, and liposomes, and seem to be a promising nonviral gene therapy vector.Keywords: aminopropyltriethoxysilane, silicon dioxide nanoparticles, Lipofectamine&reg; 2000, gene therapy vector, nanomedicineCheang TYTang BXu AWChang GQHu ZJHe WLXing ZHXu JBWang MWang SMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1061-1067 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cheang TY Tang B Xu AW Chang GQ Hu ZJ He WL Xing ZH Xu JB Wang M Wang SM Promising plasmid DNA vector based on APTES-modified silica nanoparticles
description	Tuck-yun Cheang1,, Bing Tang1,, An-wu Xu2, Guang-qi Chang1, Zuo-jun Hu1, Wei-ling He1, Zhou-hao Xing2, Jian-bo Xu1, Mian Wang1, Shen-ming Wang11Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; 2Division of Nanomaterials and Chemistry, Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, China&nbsp; *Both authors contributed equally to this workAbstract: Nanoparticles have an enormous potential for development in biomedical applications, such as gene or drug delivery. We developed and characterized aminopropyltriethoxysilane-functionalized silicon dioxide nanoparticles (APTES-SiNPs) for gene therapy. Lipofectamine&reg; 2000, a commonly used agent, served as a contrast. We showed that APTES-SiNPs had a gene transfection efficiency almost equal to that of Lipofectamine 2000, but with lower cytotoxicity. Thus, these novel APTES-SiNPs can achieve highly efficient transfection of plasmid DNA, and to some extent reduce cytotoxicity, which might overcome the critical drawbacks in vivo of conventional carriers, such as viral vectors, organic polymers, and liposomes, and seem to be a promising nonviral gene therapy vector.Keywords: aminopropyltriethoxysilane, silicon dioxide nanoparticles, Lipofectamine&reg; 2000, gene therapy vector, nanomedicine
format	article
author	Cheang TY Tang B Xu AW Chang GQ Hu ZJ He WL Xing ZH Xu JB Wang M Wang SM
author_facet	Cheang TY Tang B Xu AW Chang GQ Hu ZJ He WL Xing ZH Xu JB Wang M Wang SM
author_sort	Cheang TY
title	Promising plasmid DNA vector based on APTES-modified silica nanoparticles
title_short	Promising plasmid DNA vector based on APTES-modified silica nanoparticles
title_full	Promising plasmid DNA vector based on APTES-modified silica nanoparticles
title_fullStr	Promising plasmid DNA vector based on APTES-modified silica nanoparticles
title_full_unstemmed	Promising plasmid DNA vector based on APTES-modified silica nanoparticles
title_sort	promising plasmid dna vector based on aptes-modified silica nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/bce4bde9a396456492b272c09633261b
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Promising plasmid DNA vector based on APTES-modified silica nanoparticles

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