Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
Bacterial resistance to antibiotics is one of the largest medical concerns worldwide. One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | article |
| Lenguaje: | EN ES |
| Publicado: |
Pontificia Universidad Javeriana
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/bce519f083384edeaa3ea1cf7e53ca75 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Bacterial resistance to antibiotics is one of the largest medical concerns
worldwide. One of the bacteria of critical priority is E. coli, since
it presents different resistance mechanisms and some of its strains
have evolved resistance to beta-lactam antibiotics. We characterized 32
antibiotic resistant bacterial isolates from confirmed cases of urinary tract
infections from an array of patients in Nariño, southern Colombia. Macro
and microscopic descriptions of the 32 clinical isolates were conducted.
Resistance profiles, biochemical, and molecular characterization (via 16S
rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed.
All the isolates were identified as E. coli and had resistance to beta-lactams,
aminoglycosides, and fluoroquinolones. This resistance was related to
plasmids carrying the blaTEM, blaSHV1, and blaCTXM1 genes. There
were significant differences between the resistance proportions of the
samples (p value: 0.0000), mainly to penicillin, cefotoxin, and imipenem.
Using ERIC-PCR, four clonal states were evidenced that corroborate
a degree of genetic differentiation within the isolate set. Antibiotic
resistance observed in the isolates is associated with resistance genes
present in the bacterial chromosome and plasmids. |
|---|