Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia

Bacterial resistance to antibiotics is one of the largest medical concerns worldwide. One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic...

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Autor principal: Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon
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Publicado: Pontificia Universidad Javeriana 2020
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spelling oai:doaj.org-article:bce519f083384edeaa3ea1cf7e53ca752021-11-17T13:49:23ZCharacterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia10.11144/Javeriana.SC25-3.coar0122-74832027-1352https://doaj.org/article/bce519f083384edeaa3ea1cf7e53ca752020-12-01T00:00:00Zhttps://revistas.javeriana.edu.co/index.php/scientarium/article/view/27127https://doaj.org/toc/0122-7483https://doaj.org/toc/2027-1352Bacterial resistance to antibiotics is one of the largest medical concerns worldwide. One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic resistant bacterial isolates from confirmed cases of urinary tract infections from an array of patients in Nariño, southern Colombia. Macro and microscopic descriptions of the 32 clinical isolates were conducted. Resistance profiles, biochemical, and molecular characterization (via 16S rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed. All the isolates were identified as E. coli and had resistance to beta-lactams, aminoglycosides, and fluoroquinolones. This resistance was related to plasmids carrying the blaTEM, blaSHV1, and blaCTXM1 genes. There were significant differences between the resistance proportions of the samples (p value: 0.0000), mainly to penicillin, cefotoxin, and imipenem. Using ERIC-PCR, four clonal states were evidenced that corroborate a degree of genetic differentiation within the isolate set. Antibiotic resistance observed in the isolates is associated with resistance genes present in the bacterial chromosome and plasmids.Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen MondragonPontificia Universidad Javerianaarticleantibiotics; escherichia coli; urinary infections; plasmids and resistance.Science (General)Q1-390ENESUniversitas Scientiarum, Vol 25, Iss 3, Pp 463-488 (2020)
institution DOAJ
collection DOAJ
language EN
ES
topic antibiotics; escherichia coli; urinary infections; plasmids and resistance.
Science (General)
Q1-390
spellingShingle antibiotics; escherichia coli; urinary infections; plasmids and resistance.
Science (General)
Q1-390
Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon
Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
description Bacterial resistance to antibiotics is one of the largest medical concerns worldwide. One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic resistant bacterial isolates from confirmed cases of urinary tract infections from an array of patients in Nariño, southern Colombia. Macro and microscopic descriptions of the 32 clinical isolates were conducted. Resistance profiles, biochemical, and molecular characterization (via 16S rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed. All the isolates were identified as E. coli and had resistance to beta-lactams, aminoglycosides, and fluoroquinolones. This resistance was related to plasmids carrying the blaTEM, blaSHV1, and blaCTXM1 genes. There were significant differences between the resistance proportions of the samples (p value: 0.0000), mainly to penicillin, cefotoxin, and imipenem. Using ERIC-PCR, four clonal states were evidenced that corroborate a degree of genetic differentiation within the isolate set. Antibiotic resistance observed in the isolates is associated with resistance genes present in the bacterial chromosome and plasmids.
format article
author Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon
author_facet Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon
author_sort Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon
title Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
title_short Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
title_full Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
title_fullStr Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
title_full_unstemmed Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
title_sort characterization of antibiotic-resistant escherichia coli associated with urinary tract infections in southern colombia
publisher Pontificia Universidad Javeriana
publishDate 2020
url https://doaj.org/article/bce519f083384edeaa3ea1cf7e53ca75
work_keys_str_mv AT deisylorenaguerreroceballosedithmarielaburbanoroseroeduardoibarguenmondragon characterizationofantibioticresistantescherichiacoliassociatedwithurinarytractinfectionsinsoutherncolombia
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