Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii

Abstract Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system...

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Autores principales:	Yasuhiko Matsumoto, Tae Nagamachi, Asami Yoshikawa, Hideki Yamazaki, Yusuke Yamasaki, Tsuyoshi Yamada, Takashi Sugita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bce9a21d4fd9478684a4ad26c432d8c9
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spelling	oai:doaj.org-article:bce9a21d4fd9478684a4ad26c432d8c92021-12-02T15:15:45ZDevelopment of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii10.1038/s41598-021-97287-32045-2322https://doaj.org/article/bce9a21d4fd9478684a4ad26c432d8c92021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97287-3https://doaj.org/toc/2045-2322Abstract Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system in a highly pathogenic T. asahii strain identified using the silkworm infection model. By comparing the pathogenicity of T. asahii clinical isolates in a silkworm infection model, T. asahii MPU129 was identified as a highly pathogenic strain. Using an Agrobacterium tumefaciens-mediated gene transfer system, we obtained a T. asahii MPU129 mutant lacking the ku70 gene, which encodes the Ku70 protein involved in the non-homologous end-joining repair of DNA double-strand breaks. The ku70 gene-deficient mutant showed higher gene-targeting efficiency than the wild-type strain for constructing a mutant lacking the cnb1 gene, which encodes the beta-subunit of calcineurin. The cnb1 gene-deficient mutant showed reduced pathogenicity against silkworms compared with the parental strain. These results suggest that an efficient gene-targeting system in a highly pathogenic T. asahii strain is a useful tool for elucidating the molecular mechanisms of T. asahii infection.Yasuhiko MatsumotoTae NagamachiAsami YoshikawaHideki YamazakiYusuke YamasakiTsuyoshi YamadaTakashi SugitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yasuhiko Matsumoto Tae Nagamachi Asami Yoshikawa Hideki Yamazaki Yusuke Yamasaki Tsuyoshi Yamada Takashi Sugita Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
description	Abstract Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system in a highly pathogenic T. asahii strain identified using the silkworm infection model. By comparing the pathogenicity of T. asahii clinical isolates in a silkworm infection model, T. asahii MPU129 was identified as a highly pathogenic strain. Using an Agrobacterium tumefaciens-mediated gene transfer system, we obtained a T. asahii MPU129 mutant lacking the ku70 gene, which encodes the Ku70 protein involved in the non-homologous end-joining repair of DNA double-strand breaks. The ku70 gene-deficient mutant showed higher gene-targeting efficiency than the wild-type strain for constructing a mutant lacking the cnb1 gene, which encodes the beta-subunit of calcineurin. The cnb1 gene-deficient mutant showed reduced pathogenicity against silkworms compared with the parental strain. These results suggest that an efficient gene-targeting system in a highly pathogenic T. asahii strain is a useful tool for elucidating the molecular mechanisms of T. asahii infection.
format	article
author	Yasuhiko Matsumoto Tae Nagamachi Asami Yoshikawa Hideki Yamazaki Yusuke Yamasaki Tsuyoshi Yamada Takashi Sugita
author_facet	Yasuhiko Matsumoto Tae Nagamachi Asami Yoshikawa Hideki Yamazaki Yusuke Yamasaki Tsuyoshi Yamada Takashi Sugita
author_sort	Yasuhiko Matsumoto
title	Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
title_short	Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
title_full	Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
title_fullStr	Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
title_full_unstemmed	Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
title_sort	development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen trichosporon asahii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bce9a21d4fd9478684a4ad26c432d8c9
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Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii

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