In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

Shiyang Zhang,1 Qiming Yang,1 Weikang Zhao,1 Bo Qiao,1 Hongwang Cui,1 Jianjun Fan,2 Hong Li,3 Xiaolin Tu,4 Dianming Jiang1 1Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, 2Molecular Medicine and Cancer Research Centre, Chongqing Medical University, Chongqi...

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Autores principales:	Zhang S, Yang Q, Zhao W, Qiao B, Cui H, Fan J, Li H, Tu X, Jiang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	graphene nHA/PA66 composite biocompatibility osteogenesis bone tissue engineering Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bcea3354aac5460581aa5160feaa6e18
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spelling	oai:doaj.org-article:bcea3354aac5460581aa5160feaa6e182021-12-02T05:02:15ZIn vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material1178-2013https://doaj.org/article/bcea3354aac5460581aa5160feaa6e182016-07-01T00:00:00Zhttps://www.dovepress.com/in-vitro-and-in-vivo-biocompatibility-and-osteogenesis-of-graphene-rei-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shiyang Zhang,1 Qiming Yang,1 Weikang Zhao,1 Bo Qiao,1 Hongwang Cui,1 Jianjun Fan,2 Hong Li,3 Xiaolin Tu,4 Dianming Jiang1 1Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, 2Molecular Medicine and Cancer Research Centre, Chongqing Medical University, Chongqing, 3College of Physical Science and Technology, Sichuan University, Chengdu, 4Institutes of Life Sciences, Chongqing Medical University, Chongqing, People’s Republic of China Abstract: Graphene and its derivatives have been receiving increasing attention regarding their application in bone tissue engineering because of their excellent characteristics, such as a vast specific surface area and excellent mechanical properties. In this study, graphene-reinforced nanohydroxyapatite/polyamide66 (nHA/PA66) bone screws were prepared. The results of scanning electron microscopy observation and X-ray diffraction data showed that both graphene and nHA had good dispersion in the PA66 matrix. In addition, the tensile strength and elastic modulus of the composites were significantly improved by 49.14% and 21.2%, respectively. The murine bone marrow mesenchymal stem cell line C3H10T1/2 exhibited better adhesion and proliferation in graphene reinforced nHA/PA66 composite material compared to the nHA/PA66 composites. The cells developed more pseudopods, with greater cell density and a more distinguishable cytoskeletal structure. These results were confirmed by fluorescent staining and cell viability assays. After C3H10T1/2 cells were cultured in osteogenic differentiation medium for 7 and 14 days, the bone differentiation-related gene expression, alkaline phosphatase, and osteocalcin were significantly increased in the cells cocultured with graphene reinforced nHA/PA66. This result demonstrated the bone-inducing characteristics of this composite material, a finding that was further supported by alizarin red staining results. In addition, graphene reinforced nHA/PA66 bone screws were implanted in canine femoral condyles, and postoperative histology revealed no obvious damage to the liver, spleen, kidneys, brain, or other major organs. The bone tissue around the implant grew well and was directly connected to the implant. The soft tissues showed no obvious inflammatory reaction, which demonstrated the good biocompatibility of the screws. These observations indicate that graphene-reinforced nHA/PA66 composites have great potential for application in bone tissue engineering. Keywords: graphene, orthopedic biomaterials, cell differentiation, stem cells, bone tissue engineering Zhang SYang QZhao WQiao BCui HFan JLi HTu XJiang DDove Medical PressarticlegraphenenHA/PA66 compositebiocompatibilityosteogenesisbone tissue engineeringMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3179-3189 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	graphene nHA/PA66 composite biocompatibility osteogenesis bone tissue engineering Medicine (General) R5-920
spellingShingle	graphene nHA/PA66 composite biocompatibility osteogenesis bone tissue engineering Medicine (General) R5-920 Zhang S Yang Q Zhao W Qiao B Cui H Fan J Li H Tu X Jiang D In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
description	Shiyang Zhang,1 Qiming Yang,1 Weikang Zhao,1 Bo Qiao,1 Hongwang Cui,1 Jianjun Fan,2 Hong Li,3 Xiaolin Tu,4 Dianming Jiang1 1Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, 2Molecular Medicine and Cancer Research Centre, Chongqing Medical University, Chongqing, 3College of Physical Science and Technology, Sichuan University, Chengdu, 4Institutes of Life Sciences, Chongqing Medical University, Chongqing, People’s Republic of China Abstract: Graphene and its derivatives have been receiving increasing attention regarding their application in bone tissue engineering because of their excellent characteristics, such as a vast specific surface area and excellent mechanical properties. In this study, graphene-reinforced nanohydroxyapatite/polyamide66 (nHA/PA66) bone screws were prepared. The results of scanning electron microscopy observation and X-ray diffraction data showed that both graphene and nHA had good dispersion in the PA66 matrix. In addition, the tensile strength and elastic modulus of the composites were significantly improved by 49.14% and 21.2%, respectively. The murine bone marrow mesenchymal stem cell line C3H10T1/2 exhibited better adhesion and proliferation in graphene reinforced nHA/PA66 composite material compared to the nHA/PA66 composites. The cells developed more pseudopods, with greater cell density and a more distinguishable cytoskeletal structure. These results were confirmed by fluorescent staining and cell viability assays. After C3H10T1/2 cells were cultured in osteogenic differentiation medium for 7 and 14 days, the bone differentiation-related gene expression, alkaline phosphatase, and osteocalcin were significantly increased in the cells cocultured with graphene reinforced nHA/PA66. This result demonstrated the bone-inducing characteristics of this composite material, a finding that was further supported by alizarin red staining results. In addition, graphene reinforced nHA/PA66 bone screws were implanted in canine femoral condyles, and postoperative histology revealed no obvious damage to the liver, spleen, kidneys, brain, or other major organs. The bone tissue around the implant grew well and was directly connected to the implant. The soft tissues showed no obvious inflammatory reaction, which demonstrated the good biocompatibility of the screws. These observations indicate that graphene-reinforced nHA/PA66 composites have great potential for application in bone tissue engineering. Keywords: graphene, orthopedic biomaterials, cell differentiation, stem cells, bone tissue engineering
format	article
author	Zhang S Yang Q Zhao W Qiao B Cui H Fan J Li H Tu X Jiang D
author_facet	Zhang S Yang Q Zhao W Qiao B Cui H Fan J Li H Tu X Jiang D
author_sort	Zhang S
title	In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
title_short	In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
title_full	In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
title_fullStr	In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
title_full_unstemmed	In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
title_sort	in vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/bcea3354aac5460581aa5160feaa6e18
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In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

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