A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance

Hidekatsu Yanai1,2, Yoshiharu Tomono3, Kumie Ito1,2, Yuji Hirowatari4, Hiroshi Yoshida1,5, Norio Tada1,21Department of Internal Medicine, 2Institute of Clinical Medicine and Research, 3Department of Nutrition, 5Department of Laboratory Medicine, Jikei University School of Medicine, chiba, Japan; 4Bi...

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Autor principal:	Yanai H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	diacylglycerol energy expenditure intestine serotonin triacylglycerol Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/bcebf6482607467f86e227f0d57a604b
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spelling	oai:doaj.org-article:bcebf6482607467f86e227f0d57a604b2021-12-02T01:56:05ZA molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance1178-7007https://doaj.org/article/bcebf6482607467f86e227f0d57a604b2009-12-01T00:00:00Zhttps://www.dovepress.com/a-molecular-mechanism-for-diacylglycerol-mediated-promotion-of-negativ-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Hidekatsu Yanai1,2, Yoshiharu Tomono3, Kumie Ito1,2, Yuji Hirowatari4, Hiroshi Yoshida1,5, Norio Tada1,21Department of Internal Medicine, 2Institute of Clinical Medicine and Research, 3Department of Nutrition, 5Department of Laboratory Medicine, Jikei University School of Medicine, chiba, Japan; 4Bioscience Division, Tosoh Corporation, Kanagawa, JapanAims: A substitution of diacylglycerol (DAG) oil for triacylglycerol (TAG) oil in diet has been reported to reduce body fat and body weight, possibly by increasing postprandial energy expenditure (EE). We have previously studied plasma serotonin, which increases EE and exists in the small intestine, in individuals who ingested TAG and DAG oil, and found that DAG ingestion elevates plasma serotonin levels by about 50% compared with TAG ingestion. We studied the molecular mechanisms for DAG-mediated increase in serotonin and EE.Methods: We studied effects of 1-monoacylglycerol and 2-monoacylglycerol, distinct digestive products of DAG and TAG, respectively, on serotonin release from the Caco-2 cells (the human intestinal cell line, n = 8). Further, we studied effects of 1- and 2-monoacylglycerol, and serotonin on expression of mRNA associated with β-oxidation, FA metabolism, and thermogenesis, in the Caco-2 cells (n = 5).Results: 1-monoacylglycerol (100 µM 1-monooleyl glycerol [1-MOG]) significantly increased serotonin release from the Caco-2 cells compared with 2-monoacylglycerol (100 µM 2-MOG) by 36.6%. Expression of mRNA of acyl-CoA oxidase (ACO), fatty acid translocase (FAT), and uncoupling protein-2 (UCP-2) were significantly higher in 100 µM 1-MOG-treated Caco-2 cells than 100 µM 2-MOG-treated cells by 12.8%, 23.7%, and 35.1%, respectively. Further, expression of mRNA of ACO, medium-chain acyl-CoA dehydrogenase, FAT, and UCP-2 were significantly elevated in serotonin (400 nM)-treated Caco-2 cells compared with cells incubated without serotonin by 28.7%, 30.1%, and 39.2%, respectively.Conclusions: Our study demonstrated that 1-monoacylglycerol, a digestive product of DAG, increases serotonin release from the Caco-2 cells, and enhances expression of genes associated with β-oxidation, FA metabolism, and thermogenesis, and that serotonin increases expression of these genes, proposing a novel molecular mechanism for DAG-mediated promotion of negative caloric balance.Keywords: diacylglycerol, energy expenditure, intestine, serotonin, triacylglycerolYanai HDove Medical Pressarticlediacylglycerolenergy expenditureintestineserotonintriacylglycerolSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 3, Pp 1-6 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	diacylglycerol energy expenditure intestine serotonin triacylglycerol Specialties of internal medicine RC581-951
spellingShingle	diacylglycerol energy expenditure intestine serotonin triacylglycerol Specialties of internal medicine RC581-951 Yanai H A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
description	Hidekatsu Yanai1,2, Yoshiharu Tomono3, Kumie Ito1,2, Yuji Hirowatari4, Hiroshi Yoshida1,5, Norio Tada1,21Department of Internal Medicine, 2Institute of Clinical Medicine and Research, 3Department of Nutrition, 5Department of Laboratory Medicine, Jikei University School of Medicine, chiba, Japan; 4Bioscience Division, Tosoh Corporation, Kanagawa, JapanAims: A substitution of diacylglycerol (DAG) oil for triacylglycerol (TAG) oil in diet has been reported to reduce body fat and body weight, possibly by increasing postprandial energy expenditure (EE). We have previously studied plasma serotonin, which increases EE and exists in the small intestine, in individuals who ingested TAG and DAG oil, and found that DAG ingestion elevates plasma serotonin levels by about 50% compared with TAG ingestion. We studied the molecular mechanisms for DAG-mediated increase in serotonin and EE.Methods: We studied effects of 1-monoacylglycerol and 2-monoacylglycerol, distinct digestive products of DAG and TAG, respectively, on serotonin release from the Caco-2 cells (the human intestinal cell line, n = 8). Further, we studied effects of 1- and 2-monoacylglycerol, and serotonin on expression of mRNA associated with β-oxidation, FA metabolism, and thermogenesis, in the Caco-2 cells (n = 5).Results: 1-monoacylglycerol (100 µM 1-monooleyl glycerol [1-MOG]) significantly increased serotonin release from the Caco-2 cells compared with 2-monoacylglycerol (100 µM 2-MOG) by 36.6%. Expression of mRNA of acyl-CoA oxidase (ACO), fatty acid translocase (FAT), and uncoupling protein-2 (UCP-2) were significantly higher in 100 µM 1-MOG-treated Caco-2 cells than 100 µM 2-MOG-treated cells by 12.8%, 23.7%, and 35.1%, respectively. Further, expression of mRNA of ACO, medium-chain acyl-CoA dehydrogenase, FAT, and UCP-2 were significantly elevated in serotonin (400 nM)-treated Caco-2 cells compared with cells incubated without serotonin by 28.7%, 30.1%, and 39.2%, respectively.Conclusions: Our study demonstrated that 1-monoacylglycerol, a digestive product of DAG, increases serotonin release from the Caco-2 cells, and enhances expression of genes associated with β-oxidation, FA metabolism, and thermogenesis, and that serotonin increases expression of these genes, proposing a novel molecular mechanism for DAG-mediated promotion of negative caloric balance.Keywords: diacylglycerol, energy expenditure, intestine, serotonin, triacylglycerol
format	article
author	Yanai H
author_facet	Yanai H
author_sort	Yanai H
title	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_short	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_full	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_fullStr	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_full_unstemmed	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_sort	molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/bcebf6482607467f86e227f0d57a604b
work_keys_str_mv	AT yanaih amolecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT yanaih molecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance
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A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance

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