The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence

ABSTRACT Bacterial DNA and live bacteria have been detected in human urine in the absence of clinical infection, challenging the prevailing dogma that urine is normally sterile. Urgency urinary incontinence (UUI) is a poorly understood urinary condition characterized by symptoms that overlap urinary...

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Autores principales:	Meghan M. Pearce, Evann E. Hilt, Amy B. Rosenfeld, Michael J. Zilliox, Krystal Thomas-White, Cynthia Fok, Stephanie Kliethermes, Paul C. Schreckenberger, Linda Brubaker, Xiaowu Gai, Alan J. Wolfe
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bcf5707287ad4df3bb1fc0a48b2d5448
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spelling	oai:doaj.org-article:bcf5707287ad4df3bb1fc0a48b2d54482021-11-15T15:47:22ZThe Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence10.1128/mBio.01283-142150-7511https://doaj.org/article/bcf5707287ad4df3bb1fc0a48b2d54482014-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01283-14https://doaj.org/toc/2150-7511ABSTRACT Bacterial DNA and live bacteria have been detected in human urine in the absence of clinical infection, challenging the prevailing dogma that urine is normally sterile. Urgency urinary incontinence (UUI) is a poorly understood urinary condition characterized by symptoms that overlap urinary infection, including urinary urgency and increased frequency with urinary incontinence. The recent discovery of the urinary microbiome warrants investigation into whether bacteria contribute to UUI. In this study, we used 16S rRNA gene sequencing to classify bacterial DNA and expanded quantitative urine culture (EQUC) techniques to isolate live bacteria in urine collected by using a transurethral catheter from women with UUI and, in comparison, a cohort without UUI. For these cohorts, we demonstrated that the UUI and non-UUI urinary microbiomes differ by group based on both sequence and culture evidences. Compared to the non-UUI microbiome, sequencing experiments revealed that the UUI microbiome was composed of increased Gardnerella and decreased Lactobacillus. Nine genera (Actinobaculum, Actinomyces, Aerococcus, Arthrobacter, Corynebacterium, Gardnerella, Oligella, Staphylococcus, and Streptococcus) were more frequently cultured from the UUI cohort. Although Lactobacillus was isolated from both cohorts, distinctions existed at the species level, with Lactobacillus gasseri detected more frequently in the UUI cohort and Lactobacillus crispatus most frequently detected in controls. Combined, these data suggest that potentially important differences exist in the urinary microbiomes of women with and without UUI, which have strong implications in prevention, diagnosis, or treatment of UUI. IMPORTANCE New evidence indicates that the human urinary tract contains microbial communities; however, the role of these communities in urinary health remains to be elucidated. Urgency urinary incontinence (UUI) is a highly prevalent yet poorly understood urinary condition characterized by urgency, frequency, and urinary incontinence. Given the significant overlap of UUI symptoms with those of urinary tract infections, it is possible that UUI may have a microbial component. We compared the urinary microbiomes of women affected by UUI to those of a comparison group without UUI, using both high-throughput sequencing and extended culture techniques. We identified statistically significant differences in the frequency and abundance of bacteria present. These differences suggest a potential role for the urinary microbiome in female urinary health.Meghan M. PearceEvann E. HiltAmy B. RosenfeldMichael J. ZillioxKrystal Thomas-WhiteCynthia FokStephanie KliethermesPaul C. SchreckenbergerLinda BrubakerXiaowu GaiAlan J. WolfeAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 4 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Meghan M. Pearce Evann E. Hilt Amy B. Rosenfeld Michael J. Zilliox Krystal Thomas-White Cynthia Fok Stephanie Kliethermes Paul C. Schreckenberger Linda Brubaker Xiaowu Gai Alan J. Wolfe The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence
description	ABSTRACT Bacterial DNA and live bacteria have been detected in human urine in the absence of clinical infection, challenging the prevailing dogma that urine is normally sterile. Urgency urinary incontinence (UUI) is a poorly understood urinary condition characterized by symptoms that overlap urinary infection, including urinary urgency and increased frequency with urinary incontinence. The recent discovery of the urinary microbiome warrants investigation into whether bacteria contribute to UUI. In this study, we used 16S rRNA gene sequencing to classify bacterial DNA and expanded quantitative urine culture (EQUC) techniques to isolate live bacteria in urine collected by using a transurethral catheter from women with UUI and, in comparison, a cohort without UUI. For these cohorts, we demonstrated that the UUI and non-UUI urinary microbiomes differ by group based on both sequence and culture evidences. Compared to the non-UUI microbiome, sequencing experiments revealed that the UUI microbiome was composed of increased Gardnerella and decreased Lactobacillus. Nine genera (Actinobaculum, Actinomyces, Aerococcus, Arthrobacter, Corynebacterium, Gardnerella, Oligella, Staphylococcus, and Streptococcus) were more frequently cultured from the UUI cohort. Although Lactobacillus was isolated from both cohorts, distinctions existed at the species level, with Lactobacillus gasseri detected more frequently in the UUI cohort and Lactobacillus crispatus most frequently detected in controls. Combined, these data suggest that potentially important differences exist in the urinary microbiomes of women with and without UUI, which have strong implications in prevention, diagnosis, or treatment of UUI. IMPORTANCE New evidence indicates that the human urinary tract contains microbial communities; however, the role of these communities in urinary health remains to be elucidated. Urgency urinary incontinence (UUI) is a highly prevalent yet poorly understood urinary condition characterized by urgency, frequency, and urinary incontinence. Given the significant overlap of UUI symptoms with those of urinary tract infections, it is possible that UUI may have a microbial component. We compared the urinary microbiomes of women affected by UUI to those of a comparison group without UUI, using both high-throughput sequencing and extended culture techniques. We identified statistically significant differences in the frequency and abundance of bacteria present. These differences suggest a potential role for the urinary microbiome in female urinary health.
format	article
author	Meghan M. Pearce Evann E. Hilt Amy B. Rosenfeld Michael J. Zilliox Krystal Thomas-White Cynthia Fok Stephanie Kliethermes Paul C. Schreckenberger Linda Brubaker Xiaowu Gai Alan J. Wolfe
author_facet	Meghan M. Pearce Evann E. Hilt Amy B. Rosenfeld Michael J. Zilliox Krystal Thomas-White Cynthia Fok Stephanie Kliethermes Paul C. Schreckenberger Linda Brubaker Xiaowu Gai Alan J. Wolfe
author_sort	Meghan M. Pearce
title	The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence
title_short	The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence
title_full	The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence
title_fullStr	The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence
title_full_unstemmed	The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence
title_sort	female urinary microbiome: a comparison of women with and without urgency urinary incontinence
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/bcf5707287ad4df3bb1fc0a48b2d5448
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The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary Incontinence

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