Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat

Ghada E Ali,1 Marwa A Ibrahim,1 Ayman H El-Deeb,2 Hassan Amer,1 Said M Zaki11Cairo University, Faculty of Veterinary Medicine, Department of Biochemistry and Chemistry of Nutrition, Giza 12211, Egypt; 2Cairo University, Faculty of Veterinary Medicine, Department of Virology, Giza 12211, EgyptBackgro...

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Autores principales:	Ali GE, Ibrahim MA, El-Deeb AH, Amer H, Zaki SM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	AuNPs MiR-155 Epigenetic PROS1 TP53INP1. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bcf5c6fc90314a589d163a4fa2ef30b8
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spelling	oai:doaj.org-article:bcf5c6fc90314a589d163a4fa2ef30b82021-12-02T02:42:45ZPulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat1178-2013https://doaj.org/article/bcf5c6fc90314a589d163a4fa2ef30b82019-07-01T00:00:00Zhttps://www.dovepress.com/pulmonary-deregulation-of-expression-of-mir-155-and-two-of-its-putativ-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ghada E Ali,1 Marwa A Ibrahim,1 Ayman H El-Deeb,2 Hassan Amer,1 Said M Zaki11Cairo University, Faculty of Veterinary Medicine, Department of Biochemistry and Chemistry of Nutrition, Giza 12211, Egypt; 2Cairo University, Faculty of Veterinary Medicine, Department of Virology, Giza 12211, EgyptBackground: Gold nanoparticles (AuNPs) have been considered as an ideal candidate in various biomedical applications due to their ease of tailoring into different size, shape, and decorations with different functionalities. The current study was conducted to investigate the epigenetic alteration in the lung in response to AuNPs administration regarding microRNA-155 (miR-155) gene which can be involved in AuNP-induced lung pathogenesis.Methods: Thirty-two Wister rats were divided into two equal groups, control group and AuNPs treated group which received a single intravenous (IV) injection of plain spherical AuNPs (0.015 mg/kg body wt) with an average diameter size of 25±3 nm. Lung samples were collected from both the control and injected groups at one day, one week, one month and two months post-injection. The alteration of relative expression of miR-155 gene and two of its putative target genes; tumor protein 53 inducible nuclear protein 1 (TP53INP1) and protein S (PROS1) was investigated by real time PCR and protein S (PS) expression was analyzed by Western blotting technique.Results: The obtained results revealed that AuNPs administration significantly increases the expression level of miR-155 and reduce relative mRNA expression of TP53INP1 and PROS1 genes at one day post-injection. In contrast, a significant down-regulation of miR-155 level of expression concurrent with up-regulation of expression level of TP53INP1 and PROS1 genes were shown at one week, one month and two months post-injection. PS levels were mirrored to their PROS1 mRNA levels except for two month post-injection time point.Conclusions: These findings indicate epigenetic modulation in the lung in response to AuNPs administration regarding the miR-155 gene which can be involved in AuNP-induced lung pathogenesis.Keywords: AuNPs, MiR-155, epigenetic, PROS1, TP53INP1Ali GEIbrahim MAEl-Deeb AHAmer HZaki SMDove Medical PressarticleAuNPsMiR-155EpigeneticPROS1TP53INP1.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5569-5579 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	AuNPs MiR-155 Epigenetic PROS1 TP53INP1. Medicine (General) R5-920
spellingShingle	AuNPs MiR-155 Epigenetic PROS1 TP53INP1. Medicine (General) R5-920 Ali GE Ibrahim MA El-Deeb AH Amer H Zaki SM Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat
description	Ghada E Ali,1 Marwa A Ibrahim,1 Ayman H El-Deeb,2 Hassan Amer,1 Said M Zaki11Cairo University, Faculty of Veterinary Medicine, Department of Biochemistry and Chemistry of Nutrition, Giza 12211, Egypt; 2Cairo University, Faculty of Veterinary Medicine, Department of Virology, Giza 12211, EgyptBackground: Gold nanoparticles (AuNPs) have been considered as an ideal candidate in various biomedical applications due to their ease of tailoring into different size, shape, and decorations with different functionalities. The current study was conducted to investigate the epigenetic alteration in the lung in response to AuNPs administration regarding microRNA-155 (miR-155) gene which can be involved in AuNP-induced lung pathogenesis.Methods: Thirty-two Wister rats were divided into two equal groups, control group and AuNPs treated group which received a single intravenous (IV) injection of plain spherical AuNPs (0.015 mg/kg body wt) with an average diameter size of 25±3 nm. Lung samples were collected from both the control and injected groups at one day, one week, one month and two months post-injection. The alteration of relative expression of miR-155 gene and two of its putative target genes; tumor protein 53 inducible nuclear protein 1 (TP53INP1) and protein S (PROS1) was investigated by real time PCR and protein S (PS) expression was analyzed by Western blotting technique.Results: The obtained results revealed that AuNPs administration significantly increases the expression level of miR-155 and reduce relative mRNA expression of TP53INP1 and PROS1 genes at one day post-injection. In contrast, a significant down-regulation of miR-155 level of expression concurrent with up-regulation of expression level of TP53INP1 and PROS1 genes were shown at one week, one month and two months post-injection. PS levels were mirrored to their PROS1 mRNA levels except for two month post-injection time point.Conclusions: These findings indicate epigenetic modulation in the lung in response to AuNPs administration regarding the miR-155 gene which can be involved in AuNP-induced lung pathogenesis.Keywords: AuNPs, MiR-155, epigenetic, PROS1, TP53INP1
format	article
author	Ali GE Ibrahim MA El-Deeb AH Amer H Zaki SM
author_facet	Ali GE Ibrahim MA El-Deeb AH Amer H Zaki SM
author_sort	Ali GE
title	Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat
title_short	Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat
title_full	Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat
title_fullStr	Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat
title_full_unstemmed	Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat
title_sort	pulmonary deregulation of expression of mir-155 and two of its putative target genes; pros1 and tp53inp1 associated with gold nanoparticles (aunps) administration in rat
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/bcf5c6fc90314a589d163a4fa2ef30b8
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Pulmonary deregulation of expression of miR-155 and two of its putative target genes; PROS1 and TP53INP1 associated with gold nanoparticles (AuNPs) administration in rat

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