MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiangwei Hu, Xinqin Xiang, Wei Guan, Weihua Lou, Junming He, Jian Chen, Yin Fu, Guoliang Lou
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	miR-497-5p CDCA4 LUSC Proliferation Migration Invasion Surgery RD1-811 Anesthesiology RD78.3-87.3
Acceso en línea:	https://doaj.org/article/bcfa7ae1c27d4eefb1ad10a7f08c10d4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bcfa7ae1c27d4eefb1ad10a7f08c10d4
record_format	dspace
spelling	oai:doaj.org-article:bcfa7ae1c27d4eefb1ad10a7f08c10d42021-11-14T12:34:00ZMiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression10.1186/s13019-021-01698-21749-8090https://doaj.org/article/bcfa7ae1c27d4eefb1ad10a7f08c10d42021-11-01T00:00:00Zhttps://doi.org/10.1186/s13019-021-01698-2https://doaj.org/toc/1749-8090Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC.Jiangwei HuXinqin XiangWei GuanWeihua LouJunming HeJian ChenYin FuGuoliang LouBMCarticlemiR-497-5pCDCA4LUSCProliferationMigrationInvasionSurgeryRD1-811AnesthesiologyRD78.3-87.3ENJournal of Cardiothoracic Surgery, Vol 16, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-497-5p CDCA4 LUSC Proliferation Migration Invasion Surgery RD1-811 Anesthesiology RD78.3-87.3
spellingShingle	miR-497-5p CDCA4 LUSC Proliferation Migration Invasion Surgery RD1-811 Anesthesiology RD78.3-87.3 Jiangwei Hu Xinqin Xiang Wei Guan Weihua Lou Junming He Jian Chen Yin Fu Guoliang Lou MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
description	Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC.
format	article
author	Jiangwei Hu Xinqin Xiang Wei Guan Weihua Lou Junming He Jian Chen Yin Fu Guoliang Lou
author_facet	Jiangwei Hu Xinqin Xiang Wei Guan Weihua Lou Junming He Jian Chen Yin Fu Guoliang Lou
author_sort	Jiangwei Hu
title	MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_short	MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_full	MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_fullStr	MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_full_unstemmed	MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_sort	mir-497-5p down-regulates cdca4 to restrains lung squamous cell carcinoma progression
publisher	BMC
publishDate	2021
url	https://doaj.org/article/bcfa7ae1c27d4eefb1ad10a7f08c10d4
work_keys_str_mv	AT jiangweihu mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT xinqinxiang mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT weiguan mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT weihualou mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT junminghe mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT jianchen mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT yinfu mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression AT guolianglou mir4975pdownregulatescdca4torestrainslungsquamouscellcarcinomaprogression
_version_	1718429190776160256

MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

Ejemplares similares