Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis

J Jose Corbalan1,2, Carlos Medina1, Adam Jacoby2, Tadeusz Malinski2, Marek W Radomski11School of Pharmacy and Pharmaceutical Sciences, Faculty of Health Sciences, Panoz Institute, Trinity College Dublin, Ireland; 2Department of Chemistry and Biochemistry, Ohio University, Athens, OH, USABackground:...

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Autores principales:	Corbalan JJ, Medina C, Jacoby A, Malinski T, Radomski MW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bcfac3d845724dd19535fa09235629de
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spelling	oai:doaj.org-article:bcfac3d845724dd19535fa09235629de2021-12-02T05:02:08ZAmorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis1176-91141178-2013https://doaj.org/article/bcfac3d845724dd19535fa09235629de2012-02-01T00:00:00Zhttp://www.dovepress.com/amorphous-silica-nanoparticles-aggregate-human-platelets-potential-imp-a9216https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013J Jose Corbalan1,2, Carlos Medina1, Adam Jacoby2, Tadeusz Malinski2, Marek W Radomski11School of Pharmacy and Pharmaceutical Sciences, Faculty of Health Sciences, Panoz Institute, Trinity College Dublin, Ireland; 2Department of Chemistry and Biochemistry, Ohio University, Athens, OH, USABackground: Amorphous silica nanoparticles (SiNP) can be used in medical technologies and other industries leading to human exposure. However, an increased number of studies indicate that this exposure may result in cardiovascular inflammation and damage. A high ratio of nitric oxide to peroxynitrite concentrations ([NO]/[ONOO-]) is crucial for cardiovascular homeostasis and platelet hemostasis. Therefore, we studied the influence of SiNP on the platelet [NO]/[ONOO-] balance and platelet aggregation.Methods: Nanoparticle&ndash;platelet interaction was examined using transmission electron microscopy. Electrochemical nanosensors were used to measure the levels of NO and ONOO- released by platelets upon nanoparticle stimulation. Platelet aggregation was studied using light aggregometry, flow cytometry, and phase contrast microscopy.Results: Amorphous SiNP induced NO release from platelets followed by a massive stimulation of ONOO- leading to an unfavorably low [NO]/[ONOO-] ratio. In addition, SiNP induced an upregulation of selectin P expression and glycoprotein IIb/IIIa activation on the platelet surface membrane, and led to platelet aggregation via adenosine diphosphate and matrix metalloproteinase 2-dependent mechanisms. Importantly, all the effects on platelet aggregation were inversely proportional to nanoparticle size.Conclusions: The exposure of platelets to amorphous SiNP induces a critically low [NO]/[ONOO-] ratio leading to platelet aggregation. These findings provide new insights into the pharmacological profile of SiNP in platelets.Keywords: amorphous silica nanoparticles, nanotoxicology, nitric oxide, peroxynitrite, platelet aggregationCorbalan JJMedina CJacoby AMalinski TRadomski MWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 631-639 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Corbalan JJ Medina C Jacoby A Malinski T Radomski MW Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
description	J Jose Corbalan1,2, Carlos Medina1, Adam Jacoby2, Tadeusz Malinski2, Marek W Radomski11School of Pharmacy and Pharmaceutical Sciences, Faculty of Health Sciences, Panoz Institute, Trinity College Dublin, Ireland; 2Department of Chemistry and Biochemistry, Ohio University, Athens, OH, USABackground: Amorphous silica nanoparticles (SiNP) can be used in medical technologies and other industries leading to human exposure. However, an increased number of studies indicate that this exposure may result in cardiovascular inflammation and damage. A high ratio of nitric oxide to peroxynitrite concentrations ([NO]/[ONOO-]) is crucial for cardiovascular homeostasis and platelet hemostasis. Therefore, we studied the influence of SiNP on the platelet [NO]/[ONOO-] balance and platelet aggregation.Methods: Nanoparticle&ndash;platelet interaction was examined using transmission electron microscopy. Electrochemical nanosensors were used to measure the levels of NO and ONOO- released by platelets upon nanoparticle stimulation. Platelet aggregation was studied using light aggregometry, flow cytometry, and phase contrast microscopy.Results: Amorphous SiNP induced NO release from platelets followed by a massive stimulation of ONOO- leading to an unfavorably low [NO]/[ONOO-] ratio. In addition, SiNP induced an upregulation of selectin P expression and glycoprotein IIb/IIIa activation on the platelet surface membrane, and led to platelet aggregation via adenosine diphosphate and matrix metalloproteinase 2-dependent mechanisms. Importantly, all the effects on platelet aggregation were inversely proportional to nanoparticle size.Conclusions: The exposure of platelets to amorphous SiNP induces a critically low [NO]/[ONOO-] ratio leading to platelet aggregation. These findings provide new insights into the pharmacological profile of SiNP in platelets.Keywords: amorphous silica nanoparticles, nanotoxicology, nitric oxide, peroxynitrite, platelet aggregation
format	article
author	Corbalan JJ Medina C Jacoby A Malinski T Radomski MW
author_facet	Corbalan JJ Medina C Jacoby A Malinski T Radomski MW
author_sort	Corbalan JJ
title	Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
title_short	Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
title_full	Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
title_fullStr	Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
title_full_unstemmed	Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
title_sort	amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/bcfac3d845724dd19535fa09235629de
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Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis

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