Protein scaffolds: A tool for multi-enzyme assembly

The synthesis of complex molecules using multiple enzymes simultaneously in one reaction vessel has rapidly emerged as a new frontier in the field of bioprocess technology. However, operating different enzymes together in a single vessel limits their operational performance which needs to be address...

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Autores principales:	Shubhada Gad, Sonal Ayakar
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Protein scaffolds Multi-enzyme complex Binding modules Dockerin-cohesin interactions SpyTag-SpyCatcher system Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/bd00e60beec64a97a36ed5541179e010
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id	oai:doaj.org-article:bd00e60beec64a97a36ed5541179e010
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spelling	oai:doaj.org-article:bd00e60beec64a97a36ed5541179e0102021-11-18T04:49:12ZProtein scaffolds: A tool for multi-enzyme assembly2215-017X10.1016/j.btre.2021.e00670https://doaj.org/article/bd00e60beec64a97a36ed5541179e0102021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2215017X21000862https://doaj.org/toc/2215-017XThe synthesis of complex molecules using multiple enzymes simultaneously in one reaction vessel has rapidly emerged as a new frontier in the field of bioprocess technology. However, operating different enzymes together in a single vessel limits their operational performance which needs to be addressed. With this respect, scaffolding proteins play an immense role in bringing different enzymes together in a specific manner. The scaffolding improves the catalytic performance, enzyme stability and provides an optimal micro-environment for biochemical reactions. This review describes the components of protein scaffolds, different ways of constructing a protein scaffold-based multi-enzyme complex, and their effects on enzyme kinetics. Moreover, different conjugation strategies viz; dockerin-cohesin interaction, SpyTag-SpyCatcher system, peptide linker-based ligation, affibody, and sortase-mediated ligation are discussed in detail. Various analytical and characterization tools that have enabled the development of these scaffolding strategies are also reviewed. Such mega-enzyme complexes promise wider applications in the field of biotechnology and bioengineering.Shubhada GadSonal AyakarElsevierarticleProtein scaffoldsMulti-enzyme complexBinding modulesDockerin-cohesin interactionsSpyTag-SpyCatcher systemBiotechnologyTP248.13-248.65ENBiotechnology Reports, Vol 32, Iss , Pp e00670- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Protein scaffolds Multi-enzyme complex Binding modules Dockerin-cohesin interactions SpyTag-SpyCatcher system Biotechnology TP248.13-248.65
spellingShingle	Protein scaffolds Multi-enzyme complex Binding modules Dockerin-cohesin interactions SpyTag-SpyCatcher system Biotechnology TP248.13-248.65 Shubhada Gad Sonal Ayakar Protein scaffolds: A tool for multi-enzyme assembly
description	The synthesis of complex molecules using multiple enzymes simultaneously in one reaction vessel has rapidly emerged as a new frontier in the field of bioprocess technology. However, operating different enzymes together in a single vessel limits their operational performance which needs to be addressed. With this respect, scaffolding proteins play an immense role in bringing different enzymes together in a specific manner. The scaffolding improves the catalytic performance, enzyme stability and provides an optimal micro-environment for biochemical reactions. This review describes the components of protein scaffolds, different ways of constructing a protein scaffold-based multi-enzyme complex, and their effects on enzyme kinetics. Moreover, different conjugation strategies viz; dockerin-cohesin interaction, SpyTag-SpyCatcher system, peptide linker-based ligation, affibody, and sortase-mediated ligation are discussed in detail. Various analytical and characterization tools that have enabled the development of these scaffolding strategies are also reviewed. Such mega-enzyme complexes promise wider applications in the field of biotechnology and bioengineering.
format	article
author	Shubhada Gad Sonal Ayakar
author_facet	Shubhada Gad Sonal Ayakar
author_sort	Shubhada Gad
title	Protein scaffolds: A tool for multi-enzyme assembly
title_short	Protein scaffolds: A tool for multi-enzyme assembly
title_full	Protein scaffolds: A tool for multi-enzyme assembly
title_fullStr	Protein scaffolds: A tool for multi-enzyme assembly
title_full_unstemmed	Protein scaffolds: A tool for multi-enzyme assembly
title_sort	protein scaffolds: a tool for multi-enzyme assembly
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/bd00e60beec64a97a36ed5541179e010
work_keys_str_mv	AT shubhadagad proteinscaffoldsatoolformultienzymeassembly AT sonalayakar proteinscaffoldsatoolformultienzymeassembly
_version_	1718425063877771264

Protein scaffolds: A tool for multi-enzyme assembly

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