The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.

We report here that early apoptotic DNA fragmentation, as obtained by using an entirely new approach, is the result of an attack at a small number of specific open chromatin regions of interphase nuclei. This was demonstrated as follows: (i) chicken liver was excised and kept in sterile tubes for 1...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Miriam Di Filippo, Giorgio Bernardi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd0429ce0e6948f18a365c20ba8dca17
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd0429ce0e6948f18a365c20ba8dca17
record_format	dspace
spelling	oai:doaj.org-article:bd0429ce0e6948f18a365c20ba8dca172021-11-25T06:16:18ZThe early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.1932-620310.1371/journal.pone.0005010https://doaj.org/article/bd0429ce0e6948f18a365c20ba8dca172009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19347039/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We report here that early apoptotic DNA fragmentation, as obtained by using an entirely new approach, is the result of an attack at a small number of specific open chromatin regions of interphase nuclei. This was demonstrated as follows: (i) chicken liver was excised and kept in sterile tubes for 1 to 3 hours at 37 degrees C; (ii) this induced apoptosis (possibly because of oxygen deprivation), as shown by the electrophoretic nucleosomal ladder produced by DNA preparations; (iii) low molecular-weight DNA fragments (approximately 200 bp) were cloned, sequenced, and shown to derive predominantly from genes and surrounding 100 kb regions; (iv) a few hundred cuts were produced, very often involving the same chromosomal sites; (v) at comparable DNA degradation levels, micrococcal nuclease (MNase) also showed a general preference for genes and surrounding regions, but MNase cuts were located at sites that were quite distinct from, and less specific than, those cut by apoptosis. In conclusion, the approach presented here, which is the mildest and least intrusive approach, identifies a preferred accessibility landscape in interphase chromatin.Miriam Di FilippoGiorgio BernardiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5010 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miriam Di Filippo Giorgio Bernardi The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
description	We report here that early apoptotic DNA fragmentation, as obtained by using an entirely new approach, is the result of an attack at a small number of specific open chromatin regions of interphase nuclei. This was demonstrated as follows: (i) chicken liver was excised and kept in sterile tubes for 1 to 3 hours at 37 degrees C; (ii) this induced apoptosis (possibly because of oxygen deprivation), as shown by the electrophoretic nucleosomal ladder produced by DNA preparations; (iii) low molecular-weight DNA fragments (approximately 200 bp) were cloned, sequenced, and shown to derive predominantly from genes and surrounding 100 kb regions; (iv) a few hundred cuts were produced, very often involving the same chromosomal sites; (v) at comparable DNA degradation levels, micrococcal nuclease (MNase) also showed a general preference for genes and surrounding regions, but MNase cuts were located at sites that were quite distinct from, and less specific than, those cut by apoptosis. In conclusion, the approach presented here, which is the mildest and least intrusive approach, identifies a preferred accessibility landscape in interphase chromatin.
format	article
author	Miriam Di Filippo Giorgio Bernardi
author_facet	Miriam Di Filippo Giorgio Bernardi
author_sort	Miriam Di Filippo
title	The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
title_short	The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
title_full	The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
title_fullStr	The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
title_full_unstemmed	The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
title_sort	early apoptotic dna fragmentation targets a small number of specific open chromatin regions.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/bd0429ce0e6948f18a365c20ba8dca17
work_keys_str_mv	AT miriamdifilippo theearlyapoptoticdnafragmentationtargetsasmallnumberofspecificopenchromatinregions AT giorgiobernardi theearlyapoptoticdnafragmentationtargetsasmallnumberofspecificopenchromatinregions AT miriamdifilippo earlyapoptoticdnafragmentationtargetsasmallnumberofspecificopenchromatinregions AT giorgiobernardi earlyapoptoticdnafragmentationtargetsasmallnumberofspecificopenchromatinregions
_version_	1718414007366320128

The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.

Ejemplares similares